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2.7.7.50: mRNA guanylyltransferase

This is an abbreviated version!
For detailed information about mRNA guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.50

Reaction

GTP
+
(5')ppPur-mRNA
=
diphosphate
 +
G(5')pppPur-mRNA

Synonyms

A103R, A103R protein, cap guanylyltransferase-methyltransferase, capping enzyme, capping enzyme guanylyltransferase, Ceg1, CET1, CmCeg1, D1 protein, GDP polyribonucleotidyltransferase, GlCeg1, GTase, GTP-RNA guanylyltransferase, GTP:RNA GTase, guanylyltransferase, guanylyltransferase mRNA capping, HCE, L protein, Mce1, messenger RNA guanylyltransferase, More, mRNA capping enzyme, mRNA guanylyl transferase, mRNA-cap, mRNA-capping enzyme, NAD-decapping enzyme, nsP1, NudC, PRNTase, protein lambda2, RNA capping enzyme, RNA guanylyltransferase, RNGTT, TbCgm1, TTM-type RTPase-GTase, VACWR106, VP3

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.50 mRNA guanylyltransferase

Storage Stability

Storage Stability on EC 2.7.7.50 - mRNA guanylyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 50% glycerol, 0.1 mg/ml gelatin, enzyme concentration: 0.1 mg/ml, stable
-
-20°C, stable for more than 6 months, considerably longer at -70°C
-
-70°C, stable for at least 9 months
-
-80°C, reaction intermediate enzyme-GMP, purified, stable for at least several weeks
-
-80°C, stable for at least 6 months
-
0°C, 48 h, 90% loss of activity, 59000 MW protein which lacks 7-methyltransferase activity
-
4°C or -20°C, purified enzyme, stable for several months
-
4°C, stable for more than 6 months
-