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2.7.7.52: RNA uridylyltransferase

This is an abbreviated version!
For detailed information about RNA uridylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.52

Reaction

UTP
+
RNAn
=
diphosphate
 +
RNAn+3'-U

Synonyms

3' terminal uridylyl transferase, 3'-terminal uridylyl transferase 1, At2g45620, caffeine-induced death suppressor protein 1, Cid1, GLD2, HESO1, MEAT1, mitochondrial editosome-like complex associated TUTase 1, More, RET, RET1, RET2, RNA editing TUTase, RNA silencing protein 1, Rsp1, Tailor, TbMP57 TUTase, TbTUT3, TbTUT4, terminal RNA uridylyltransferase, terminal U transferase (TUTase), terminal uridylyl transferase, terminal uridylyltransferase, terminal uridylyltransferase Tailor, TNTase CG1091, TUT, TUT1, TUT2, TUT4, TUT7, TUTase, TUTase 1, TUTase 2, TUTase 3, TUTase 4, TUTase4, TUTase6, TUTase7, U6 snRNA-specific terminal uridylyltransferase, U6-TUTase, uridylyltransferase, terminal, URT1, UTP: RNA uridylyltransferase, UTP:RNA uridylyltransferase 1, Zcchc11, ZCCHC6

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.52 RNA uridylyltransferase

Purification

Purification on EC 2.7.7.52 - RNA uridylyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2400fold to near homogeneity
-
614fold from uninfected and 117fold from CPMV-infected leaves
-
expression and purification of 20S editosome-associated RET2 complex from Trypanosoma brucei by affinity isolation
-
homogenous RET1 is purified from an enriched mitochondrial fraction of Leishmania tarentolae by a series of chromatographic steps: affinity chromatography (polyU Sepharose 4B), anion-exchange chromatography (Poros 20 HQ 46/100 column), gel filtration (Superose 12 and Superose 6 columns) and hydrophobic interactions chromatography (Phenyl Superose column)
-
Ni-NTA agarose column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography and Superdex 200 gel filtration
partial purification of U6 snRNA specific enzyme form via chromatography on a U6 snRNA affinity resin, separation from unspecific enzyme form
-
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli
-
recombinant His-tagged wild-type and mutant TUT4 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ion exchange chromatography, and gel filtration
recombinant RET1 from Leishmania tarentolae is purified by cation-exchange resin (Sepharose S Fast Flow), metal affinity chromatography (Talon metal affinity column), anion-exchange chromatography (Mono Q 5/5 column), expected yield: 0.2-0.5 mg/liter
-
recombinant RET1 from Trypanosoma brucei is purified by metal affinity chromatography (Talon metal affinity column), anion-exchange chromatography (HiTrap Q column) and loading onto a MonoS 5/5 column (final purification step), expected yield is 0.5 mg/liter of bacterial culture, it is important to maintain the concentration of KCl above 80 mM during purification, as protein tends to precipitate under low salt conditions
-
recombinant TAP-tagged enzyme from Leishmania tarentolae cells
-
the recombinant expressed RET2 is purified by loading the protein extract onto a Talon affinity column and onto a HiTrap Sepharose S column, the expected protein yield is 1 mg of protein per liter of bacterial culture
-