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2.7.7.6: DNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about DNA-directed RNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.6

Reaction

nucleoside triphosphate
+
RNAn
=
diphosphate
 +
RNAn+1

Synonyms

C RNA formation factors, chloroplast soluble RNA polymerase, deoxyribonucleic acid-dependent ribonucleic acid polymerase, DNA-dependent ribonucleate nucleotidyltransferase, DNA-dependent RNA nucleotidyltransferase, DNA-dependent RNA polymerase, DNA-dependent RNA polymerase I, DNA-dependent RNA polymerase III, DNA-dependent RNAP, h-mtRNAP, K1E RNAP, mitochondrial RNA polymerase, mitoRNAP, More, mtRNAP, multi-subunit RNA polymerase, nucleotidyltransferase, ribonucleate, plastid RNA polymerase, plastid-encoded polymerase, plastid-encoded RNA polymerase, Pol I, Pol II, pol III, Pol IIIalpha, Pol IIIbeta, Pol IV, Pol V, polI, PolIII, POLRMT, ribonucleate nucleotidyltransferase, ribonucleate polymerase, ribonucleic acid formation factors, C, ribonucleic acid nucleotidyltransferase, ribonucleic acid polymerase, ribonucleic acid transcriptase, ribonucleic polymerase, ribonucleic transcriptase, rifampicin-resistant RNA polymerase, RNA formation factors, C, RNA nucleotidyltransferase, RNA nucleotidyltransferase (DNA-directed), RNA pol III, RNA polymerase, RNA polymerase core enzyme, RNA polymerase I, RNA polymerase II, RNA polymerase II complex, RNA polymerase III, RNA polymerase III complex, RNA transcriptase, RNAP, RNAP core enzyme, RNAP I, RNAP II, RNAP III, RNAP sigma70, RNAP-II, RNAP2, RNAPII, RPO, rpo1N, Rpo41, RpoA, RpoD, RpoS, RpoT, Saci_0834, sigma38 RNA polymerase, sigmaS-containing RNA polymerase, T7 RNA polymerase, T7 RNAP, T7-like RNA polymerase, Taq polymerase, transcriptase, YonO

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.6 DNA-directed RNA polymerase

Activating Compound

Activating Compound on EC 2.7.7.6 - DNA-directed RNA polymerase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CK2
-
Ctk1
-
the kinase is required for the stability of the scaffold, but Ctk1 kinase activity is not required for the dissociation of basal transcription factors
-
glutamate
-
glutamate remodels the sigma38 transcription complex for activation. Accumulation of the simple signaling molecule glutamate can reprogram RNA polymerase in vitro without the need for specific protein receptors. During osmotic activation, glutamate appears to act as a Hofmeister series osmolyte to facilitate promoter escape. Escape is accompanied by a remodeling of the key interaction between the sigma38 stress protein and the beta-flap of the bacterial core RNA polymerase. This activation event contrasts with the established mechanism of inhibition in which glutamate, by virtue of its electrostatic properties, helps to inhibit binding to ribosomal promoters after osmotic shock
histone-like nucleoid structuring protein
-
i.e. H-NS, H-NS stimulates transcription from the F3 fragment, it can facilitate specific DNA-binding by RNA polymerase in AT-rich gene regulatory regions. Correct positioning of RNA polymerase at PehxCABD requires H-NS. Footprint of RNA polymerase (s70 RC461-FeBABE) interactions with -10 elements in the ehxCABD regulatory region in the presence of H-NS, overview
-
PAF53
-
potassium acetate
-
activates
potassium aspartate
-
activates
potassium chloride
-
activates
potassium glutamate
-
activates highly, role of potassium ion in the activation of osmotic transcription
potassium nitrate
-
activates
Rho
-
RNase P
-
required for Pol I and Pol III required
-
sigma factor
-
sigma70
-
the sigma factor increases the transcription efficiency of templates with nonphysiological nonprokaryotic promoters
-
spermidine
Spt5
-
the large subunit of the DRB sensitivity-inducing factor, DSIF, represses or activates RNAPII elongation in vitro. CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo, overview. Mutant NSpt5, lacking the C-terminus, directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b, histone H3 Lys 4 trimethylation, and surprisingly, the negative elongation factor A at the locus, indicating a direct action of NSpt5 on the elongation repressed locus, nuclear extracts containing the constitutively active P-TEFb and WT DSIF lead to a time-dependent increase of the long, promoter-distal RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5, overview
-
Spt6
-
transcription factor, Pol II shows a broad requirement for essential Spt6 during different stages of development, e.g. for for maximal recruitment of Paf1 and Spt5 to transcriptionally active Hsp70. Spt6 interacts with both nucleosome structure and Pol II, it has a role in elongation, directed RNAi knock-down of Spt6 reduces the elongation rate, the Spt6-dependent effect on elongation rate persists during steady-state-induced transcription, reducing the elongation rate from about 1100 to 500 bp/min. Stimulation of Pol II elongation rate by Spt6 is not mediated through transcription factor TFIIS
-
TAFI protein
-
TFB2
-
the essential initiation factor forms a network of interactions with DNA near the transcription start site and facilitates promoter melting but may not be essential for promoter recognition, TFB2 bridges upstream and downstream promoter contacts of the initiation complex, mapping of TFB2-DNA interactions at the transcription start site, overview
-
TFB2M
-
the requirement for TFB2M in transcription of dsDNA is that it can stabilize an incompletely single-stranded template established by negative supercoiling
-
TFIIIE
-
a basal transcription factor, complexes with several ribosomal proteins and enhances tRNA and 5S rRNA transcription of the RNA polymerase, regualtion, overview
-
TFIIS
-
an RNA cleavage stimulatory factor TFIIS. TFIIS can rescue an arrested polymerase by creating a new RNA 3' end at the active site from which transcription can resume, mechanism, overview
-
thermine
-
optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine
TIF-IB/SL 1
-
transcription factor TFIIIB
-
proper initiation by RNA pol III requires the transcription factor TFIIIB. Gene-external U6 snRNA transcription requires TFIIIB consisting of Bdp1, TBP, and Brf2. Transcription from the gene internal tRNA promoter requires TFIIIB composed of Bdp1, TBP, and Brf1. Breast cancer susceptibility gene 1, BRCA1, inhibits TFIIB, which interacts with the BRCA1 C-terminal region domain of Fcp1p, an RNA polymerase II phosphatase, TFIIIB regulation network, overview
-
upstream binding factor
-
additional information
-