2.7.7.6: DNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about DNA-directed RNA polymerase, go to the full flat file.

Reaction

Synonyms

C RNA formation factors, chloroplast soluble RNA polymerase, deoxyribonucleic acid-dependent ribonucleic acid polymerase, DNA-dependent ribonucleate nucleotidyltransferase, DNA-dependent RNA nucleotidyltransferase, DNA-dependent RNA polymerase, DNA-dependent RNA polymerase I, DNA-dependent RNA polymerase III, DNA-dependent RNAP, h-mtRNAP, K1E RNAP, mitochondrial RNA polymerase, mitoRNAP, More, mtRNAP, multi-subunit RNA polymerase, nucleotidyltransferase, ribonucleate, plastid RNA polymerase, plastid-encoded polymerase, plastid-encoded RNA polymerase, Pol I, Pol II, pol III, Pol IIIalpha, Pol IIIbeta, Pol IV, Pol V, polI, PolIII, POLRMT, ribonucleate nucleotidyltransferase, ribonucleate polymerase, ribonucleic acid formation factors, C, ribonucleic acid nucleotidyltransferase, ribonucleic acid polymerase, ribonucleic acid transcriptase, ribonucleic polymerase, ribonucleic transcriptase, rifampicin-resistant RNA polymerase, RNA formation factors, C, RNA nucleotidyltransferase, RNA nucleotidyltransferase (DNA-directed), RNA pol III, RNA polymerase, RNA polymerase core enzyme, RNA polymerase I, RNA polymerase II, RNA polymerase II complex, RNA polymerase III, RNA polymerase III complex, RNA transcriptase, RNAP, RNAP core enzyme, RNAP I, RNAP II, RNAP III, RNAP sigma70, RNAP-II, RNAP2, RNAPII, RPO, rpo1N, Rpo41, RpoA, RpoD, RpoS, RpoT, Saci_0834, sigma38 RNA polymerase, sigmaS-containing RNA polymerase, T7 RNA polymerase, T7 RNAP, T7-like RNA polymerase, Taq polymerase, transcriptase, YonO

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.6 DNA-directed RNA polymerase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
56	Activating Compound
385907	AA Sequence
15	Application
1	CAS Registry Number
77	Cloned(Commentary)
1	Cofactor
34	Crystallization (Commentary)
4851	Disease/ Diagnostics
5	Expression
117	General Information
5	General Stability
8	IC50 Value
164	Inhibitors
44	KM Value [mM]
52	Localization
67	Metals/Ions
122	Molecular Weight [Da]
84	Natural Substrates/ Products (Substrates)
551	Organism
1	Oxidation Stability
4123	PDB ID
29	pH Optimum
3	pH Range
20	Posttranslational Modification
77	Protein Variants
50	Purification (Commentary)
3	Reaction
3	Reaction Type
312	Reference
2	Renatured (Commentary)
67	Source Tissue
26	Specific Activity [micromol/min/mg]
3	Storage Stability
245	Substrates and Products (Substrate)
100	Subunits
403	Synonyms
1	Systematic Name
26	Temperature Optimum [°C]
4	Temperature Range [°C]
8	Temperature Stability [°C]
22	Turnover Number [1/s]

Application

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Application on EC 2.7.7.6 - DNA-directed RNA polymerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Application Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

Escherichia coli

-

assay for RNA polymerase activity that uses the dye RiboGreen to detect transcripts by fluorescence and is thus free of the expense, short shelf life, and high handling costs of radioisotopes. The method is relatively quick and can be performed entirely in microplate formate, allowing for the processing of dozens to hundreds of samples in parallel

660691

diagnostics

Lamium

-

evaluation of rpo genes as phylogenetic markers on the example of the genus Lamium, Lamiaceae. The most promising phylogenetic marker is the rpoA gene, while the least useful gene appears to be rpoC1

738415

drug development

3 entries

medicine

Escherichia coli

-

the enzyme is a promising target for the discovery of new antimicrobial agents

673217

molecular biology

4 entries

synthesis

5 entries

drug development

Mus musculus

-

development of drugs that target the Pol I transcription machinery at different points for use in cancer therapies, overview

701717

drug development

Homo sapiens

-

development of drugs that target the Pol I transcription machinery at different points for use in cancer therapies, overview

701717

drug development

Thermus thermophilus

-

the enzyme is a target for drug design

703147

molecular biology

Saccharomyces cerevisiae

-

Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA

738689

molecular biology

Acanthamoeba polyphaga Mimivirus

Q5UQ32; E3VZD2

usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies

738334

molecular biology

Phaeocystis globosa virus

R4THW7; R4TFI0

usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies

738334

molecular biology

Phytophthora parasitica virus

-

usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies

738334

synthesis

Escherichia coli

-

the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNA and labeled RNA probes, method evaluation, overview

703357

synthesis

Escherichia phage T7

-

the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNA and labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage T7 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding T7 promoter, applications overview

703357

synthesis

Zindervirus SP6

-

the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNAand labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage SP6 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding SP6 promoter, applications overview

703357

synthesis

Enterobacteria phage T3

-

the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNAand labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage T3 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding T3 promoter, applications overview

703357

synthesis

Clostridioides difficile

-

development of an in vitro transcription system that accurately initiates transcription from bona fide promoters of the Peptoclostridium difficile glutamate dehydrogenase gene and rRNA genes rrnC and rrnE operons

727744