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2.7.7.6: DNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about DNA-directed RNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.6

Reaction

nucleoside triphosphate
+
RNAn
=
diphosphate
 +
RNAn+1

Synonyms

C RNA formation factors, chloroplast soluble RNA polymerase, deoxyribonucleic acid-dependent ribonucleic acid polymerase, DNA-dependent ribonucleate nucleotidyltransferase, DNA-dependent RNA nucleotidyltransferase, DNA-dependent RNA polymerase, DNA-dependent RNA polymerase I, DNA-dependent RNA polymerase III, DNA-dependent RNAP, h-mtRNAP, K1E RNAP, mitochondrial RNA polymerase, mitoRNAP, More, mtRNAP, multi-subunit RNA polymerase, nucleotidyltransferase, ribonucleate, plastid RNA polymerase, plastid-encoded polymerase, plastid-encoded RNA polymerase, Pol I, Pol II, pol III, Pol IIIalpha, Pol IIIbeta, Pol IV, Pol V, polI, PolIII, POLRMT, ribonucleate nucleotidyltransferase, ribonucleate polymerase, ribonucleic acid formation factors, C, ribonucleic acid nucleotidyltransferase, ribonucleic acid polymerase, ribonucleic acid transcriptase, ribonucleic polymerase, ribonucleic transcriptase, rifampicin-resistant RNA polymerase, RNA formation factors, C, RNA nucleotidyltransferase, RNA nucleotidyltransferase (DNA-directed), RNA pol III, RNA polymerase, RNA polymerase core enzyme, RNA polymerase I, RNA polymerase II, RNA polymerase II complex, RNA polymerase III, RNA polymerase III complex, RNA transcriptase, RNAP, RNAP core enzyme, RNAP I, RNAP II, RNAP III, RNAP sigma70, RNAP-II, RNAP2, RNAPII, RPO, rpo1N, Rpo41, RpoA, RpoD, RpoS, RpoT, Saci_0834, sigma38 RNA polymerase, sigmaS-containing RNA polymerase, T7 RNA polymerase, T7 RNAP, T7-like RNA polymerase, Taq polymerase, transcriptase, YonO

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.6 DNA-directed RNA polymerase

Cloned

Cloned on EC 2.7.7.6 - DNA-directed RNA polymerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminal domain encoding DNA and amino acid sequence determination and analysis, genotyping, sequence comparisons of the CTD region from 36 yeast strains. The DNA helicase Pif1 suppresses CTD rearrangement
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cloning of the cDNA for the mtRNAP of Physarum and expression in Escherichia coli
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cloning of the genes for the three large subunits A, B and C, which are organized in line in the order B-A-C, genetic organization, DNA and amino acid sequence determination and anaylsis, the three RNA polymerase subunit genes are cotranscribed together with an ORF of 88 amino acid residues length situated immediately upstream of the B gene and two ORFs of 104 and 130 amino acid residues following the C gene, overview
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construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter
expressed in Escherichia coli as an N-terminal maltose binding protein (MBP) fusion protein
expressed in HEK-293T cells, HAT-1080 cells, and Magi cells
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expression in a phage display using Halobacterium phage phi H, method overview
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expression in Escherichia coli
expression in Saccharomyces cerevisiae
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expression of C-terminally His-tagged alpha subunit-encoded by gene rpoA, and coexpression of the subunits of Xanthomonas campestris pv. campestris core RNAP from plasmid pBBad22K in Escherichia coli strain BL21(DE3), overexpression with or without omega
expression of His-tagged subunits PA and PB1 in Escherichia coli
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expression of the N-terminally His6-tagged intein fusion mitoRNAP protein
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expression of the T7 RNA polymerase tagged with yellow fluorescent protein and His6 in Rhodobacter capsulatus strain B10S establishing an additional expression system for the enzyme, method optimization and system evaluation, comparison to the Escherichia coli strain BL21(DE3) expression system, both using the expression vector pRhotHi-2-yfp-His6, overview. T7 RNA polymerase subcloning in Escherichia coli strain DH5alpha. Quantitative expression analysis, overview
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gene k1ep, expression of His6-tagged K1E in Escherichia coli strain bL21(DE3), recombinant expression of K1E RNA polymerase in Bacillus megaterium strain MS941, a nprM deletion mutant of DSM319, using K1E promoters or SP6 promoter and a PEG-mediated transformation, method development and evaluation. The T7 RNAP promoter is not recognized at all
Vectrevirus K1E
gene rpoA, library screening
-
gene rpoA, recombinant expression of the C-terminally His9-tagged enzyme in Escherichia coli B834 (DE3) cells, subcloning in Escherichia coli strain DH5alpha
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gene rpoA, recombinant expression of the HA-tagged alpha-subunit in transplastomic tobacco plants, analysis of the distribution of the tagged polymerase in plastid subfractions, and associated genes are identified under various light conditions. RpoA:HA is detected as early as the 3rd day after imbibition, and is constitutively expressed in green tissue over 60 days of plant development. Phenotypes of tagged RpoA and wild-type control lines, overview. Transplastomic tobacco plants grown on soil are phenotypically indistinguishable from wild-type
gene rpoB encodes the RNA polymerase beta subunit, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of rpoB
gene rpoB, encoding the beta subunit of the DNA-directed RNA polymerase of phytoplasma, genetic structure, genotyping and phylogenetic analysis, detailed overview
gene rpoT, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure and phylogenetic analysis, N-terminal transit peptide of SmRpoT confers targeting of green fluorescent-tagged protein exclusively to mitochondria after transient expression in Arabidopsis thaliana and Selaginella moellendorffii protoplasts
gene TON-0309 encoding DNA-directed RNA polymerase subunit L, recombinant overexpression of subunit L in Escherichia coli strain BL21(DE3)
genes encoding subunits of the enzyme, DNA and amino acid sequence determination and analysis, genetic organization involving the genes of the transcription machinery, i.e. gene tfis
genes for the subunits beta and beta‘
-
genes rpoA, rpoB, rpoC1, and rpoC2 encoding the enzyme subunits, DNA and amino acid sequence determination and analysis, phylogenetic analysis, number of sites containing synonymous and nonsynonymous substitutions, overview
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genes rpoE2 and rpoE3, phage library screening, DNA and amino acid sequence determination and analysis, genetic organization and associated sigma factors, phylogenetic analysis of the sigma factors, overview
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identification and sequencing of the SK7 promoter, sequence comparison of the porcine SK7 snRNA with sequences from other organisms, overview
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isolation and characterisation of a cDNA encoding the RNA polymerase common subunit RPB6
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mitochondrial plasmid sequence determination and analysis, the viral-like RNA polymerase ORF has 3' and 5' inverted terminal repeats, also a 5' binding protein. This protein can be involved in both replication and integration processes of these plasmid in the mitochondrial genome
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overexpression of His6-tagged subunit D of the RNA polymerase. A shuttle expression vector system for Pyrococcus furiosus and Escherichia coli is described allowing the regulated expression of proteins in Pyrococcus. A shuttle vector of Pyrococcus abyssi is redesigned using the overexpression of the HMG-CoA reductase as a selection marker which confers resistance to the antibiotic simvastatin
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Production of different translational and transcriptional fusions in Escherichia coli. PCR fragments of different sizes are cloned into plasmids resulting in intermediate constructs.
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recombinant expression of wild-type ProtA-tagged enzyme PolI in strain y2423. The second largest subunit of one polymerase is expressed as a C-terminal fusion protein with a protein A tag. Between the C terminus of the subunit and the protein A part, a recognition site for TEV protease is located
-
recombinant Rpb2 R512C, TAP-tagged at the C-terminus of the RNAP II Rpb9 subunit
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RNAPsubunit 2, RNAP2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis and tree. Reconstruction of putative ancestral RNAP2 from the genome of eukaryote Hydra magnipapillata and Phytophthora parasitica using viral RNAP2, detection of megaviruse DNAs that are integrated into eukaryote genomes and misclassified
RPB2 gene encoding the Rpb2 subunit of yeast RNAP II, expression of mutant enzyme and subunits in Escherichia coli strain XL-1 Blue
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rpoB(R) markedly activates antibiotic biosynthesis in the wild-type Streptomyces lividans strain 1326 and also in strain KO-421, a relaxed mutant unable to produce ppGpp, phenotypes, overview
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stable ectopic expression of subunit RPC32alpha in IMR90 fibroblasts
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subunit 3 gene, rpb3
-
the genes for the large subunits beta and beta‘ are cloned and sequenced. MW for beta‘ and beta, calculated from nucleotide sequence is 190522 Da and 143139 Da
-
the genes rpoA, rpoB and rpoC which encode the RNA polymerase, alpha-, beta- and beta‘subunits, respectively, have been individually placed on expression plasmids under control of the bacteriophage T7 promoter. Induction of the T7 RNA polymerase gene in hist cells harbouring each of the three plasmids, results in the extensive overproduction of the three polypeptides
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