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2.7.7.6: DNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about DNA-directed RNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.6

Reaction

nucleoside triphosphate
+
RNAn
=
diphosphate
 +
RNAn+1

Synonyms

C RNA formation factors, chloroplast soluble RNA polymerase, deoxyribonucleic acid-dependent ribonucleic acid polymerase, DNA-dependent ribonucleate nucleotidyltransferase, DNA-dependent RNA nucleotidyltransferase, DNA-dependent RNA polymerase, DNA-dependent RNA polymerase I, DNA-dependent RNA polymerase III, DNA-dependent RNAP, h-mtRNAP, K1E RNAP, mitochondrial RNA polymerase, mitoRNAP, More, mtRNAP, multi-subunit RNA polymerase, nucleotidyltransferase, ribonucleate, plastid RNA polymerase, plastid-encoded polymerase, plastid-encoded RNA polymerase, Pol I, Pol II, pol III, Pol IIIalpha, Pol IIIbeta, Pol IV, Pol V, polI, PolIII, POLRMT, ribonucleate nucleotidyltransferase, ribonucleate polymerase, ribonucleic acid formation factors, C, ribonucleic acid nucleotidyltransferase, ribonucleic acid polymerase, ribonucleic acid transcriptase, ribonucleic polymerase, ribonucleic transcriptase, rifampicin-resistant RNA polymerase, RNA formation factors, C, RNA nucleotidyltransferase, RNA nucleotidyltransferase (DNA-directed), RNA pol III, RNA polymerase, RNA polymerase core enzyme, RNA polymerase I, RNA polymerase II, RNA polymerase II complex, RNA polymerase III, RNA polymerase III complex, RNA transcriptase, RNAP, RNAP core enzyme, RNAP I, RNAP II, RNAP III, RNAP sigma70, RNAP-II, RNAP2, RNAPII, RPO, rpo1N, Rpo41, RpoA, RpoD, RpoS, RpoT, Saci_0834, sigma38 RNA polymerase, sigmaS-containing RNA polymerase, T7 RNA polymerase, T7 RNAP, T7-like RNA polymerase, Taq polymerase, transcriptase, YonO

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.6 DNA-directed RNA polymerase

Purification

Purification on EC 2.7.7.6 - DNA-directed RNA polymerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromatography
-
IgG-Sepharose beads chromatography
-
partial
PCC 7120
-
polyethylene imine precipitation, anion exchange chromatography, heparin and T4-DNA affinity chronatography, and sucrose gradient centrifugation
-
recombinant C-terminally His9-tagged enzyme to 90% purity by nickel affinity chromatography
-
recombinant enzymes partially from Escherichia coli
recombinant His-tagged subunits PA and PB1 from Escherichia coli by nickel affinity chromatography
-
recombinant His6-tagged K1E from Escherichia coli strain BL21(DE3) by nickel affinity chromatgraphy, dialysis and ultrafiltration
Vectrevirus K1E
recombinant RNAP subunit L from Escherichia coli strain BL21(DE3) by anion exchange chromatography and ultrafiltration
recombinant wild-type and mutant RNAP II proteins
-
recombinant wild-type ProtA-tagged enzyme PolI from strain y2423 by protein A affinity chromatography and cleavage of the ProtA tag by TEV protease
-
recombinant Xcc core RNAP subunits from Escherichia coli by a two-step chromatographic process
RNA polymerase II
-
RNA polymerase III subunits isolation from A-431 cells and placenta cell nuclei by anion exchange chromatography and ultrafiltration
-
RNA polymerase III subunits isolation from fibroblast nuclei by anion exchange chromatography and ultrafiltration
-
RNA polymerase/N-terminal maltose binding protein (MBP) fusion protein
RNAP purification from iron-starved Pseudomonas aeruginosa cells
-
RNAP rom Mycobacterium smegmatis from exponential phase
-