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2.7.7.7: DNA-directed DNA polymerase

This is an abbreviated version!
For detailed information about DNA-directed DNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.7

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

3'–5'-exonuclease, ABO4/POL2a/TIL1, Afu polymerase, ASFV DNA polymerase, ASFV Pol X, B-family replicative DNA polymerase, beta type DNA polymerase, Bst DNA polymerase, CpDNApolI, DBH, Dbh DNA polymerase, Dbh polymerase, ddNTP-sensitive DNA polymerase, Deep Vent DNA polymerase, DeepVent DNA polymerase, deoxynucleate polymerase, deoxyribonucleate nucleotidyltransferase, deoxyribonucleic acid duplicase, deoxyribonucleic acid polymerase, deoxyribonucleic duplicase, deoxyribonucleic polymerase, deoxyribonucleic polymerase I, DinB DNA polymerase, DinB homologue, Dmpol zeta, DNA deoxynucleotidyltransferase, DNA duplicase, DNA nucleotidyltransferase, DNA nucleotidyltransferase (DNA-directed), DNA pol, DNA pol B1, DNA Pol eta, DNA Pol lambda, DNA pol NI, DNA pol Y1, DNA polmerase beta, DNA polymerase, DNA polymerase 1, DNA polymerase 2, DNA polymerase 4, DNA polymerase A, DNA polymerase alpha, DNA polymerase B, DNA polymerase B1, DNA polymerase B2, DNA polymerase B3, DNA polymerase beta, DNA polymerase D, DNA polymerase Dbh, DNA polymerase delta, DNA polymerase Dpo4, DNA polymerase epsilon, DNA polymerase eta, DNA polymerase gamma, DNA polymerase I, DNA polymerase II, DNA polymerase III, DNA polymerase III epsilon subunit, DNA polymerase iota, DNA polymerase IV, DNA polymerase kappa, DNA polymerase lambda, DNA polymerase mu, DNA polymerase ny, DNA polymerase pyrococcus kodakaraensis, DNA polymerase theta, DNA polymerase V, DNA polymerase X, DNA polymerase zeta, DNA polymerases B, DNA polymerases D, DNA polymmerase I, DNA primase-polymerase, DNA replicase, DNA replication polymerase, DNA-dependent DNA polymerase, DNAP, DP1Pho, DP2Pho, Dpo1, Dpo2, Dpo3, Dpo4, Dpo4 polymerase, Dpo4-like enzyme, duplicase, error-prone DNA polymerase, error-prone DNA polymerase X, family B-type DNA polymerase, hoPolD, HSV 1 POL, Igni_0062, K4 polymerase, K4pol, K4PolI, kDNA replication protein, KDO XL DNA polymerase, KF(exo-), KF-, Klenow fragment, Klenow-like DNA polymerase I, KOD DNA polymerases, lesion-bypass DNA polymerase, M1 DNA polymerase, M1pol, MacDinB-1, MA_4027, Miranda pol beta protein, mitochondrial DNA polymerase, Mka polB, More, MsDpo4, mtDNA polymerase NI, mtDNA replicase, Neq DNA polymerase, non-replicative DNA polymerase III, nucleotidyltransferase, deoxyribonucleate, OsPOLP1, PabPol D, PabpolB, PabpolD, Pfu, Pfu DNA polymerase, Pfu Pol, Pfu-POl, PH0121, PH0123, phi29 DNA polymerase, phi29 DNApol, PhoPolD, phPol D, Pol, pol alpha, Pol B, Pol B1, pol beta, Pol BI, pol delta, pol E, POl epsilon, Pol eta, Pol gamma, Pol I, Pol II, pol III, pol iota, Pol IV, pol kappa, pol kappaDELTAC, Pol lambda, Pol mu, pol NI, Pol ny, Pol theta, Pol V, pol Vent (exo-), Pol X, Pol zeta, Pol-beta, POL1, Pol2, POL2a, Pol3, Pol31, PolB, POlB1, polbeta, polD, POLD4, Poldelta, POLdelta1, PolDPho, Polepsilon, Poleta, POLG, PolH, polI, POLIB, POLIC, POLID, poliota, Polkappa, PolX, PolY, poly iota, polymerase alpha catalytic subunit A, polymerase III, pORF30, Pwo DNA polymerase, R2 polymerase, R2 reverse transcriptase, R2-RT, RAD30, RB69 DdDp, RB69 DNA Polymerase, RB69pol, Rec1, repair polymerase, replicative DNA polymerase, reverse transcriptase, RKOD DNA polymerase, Rv1537, Rv3056, Saci_0554, sequenase, Sso, Sso DNA pol B1, Sso DNA pol Y1, Sso DNA polymerase Y1, Sso DNApol, Sso pol B1, SSO0552, SSO2448, SsoDpo1, SsoPolB1, SsoPolY, Szi DNA polymerase, T4 DNA polymerase, T7 DNA polymerase, Taq DNA polymerase, Taq Pol I, Taq polymerase, Tba5 DNA polymerase, Tca DNA polymerase, Tga PolB, TGAM_RS07365, Tkod-Pol, translesion DNA polymerase, translesion DNA synthesis polymerase, translesion polymerase Dpo4, UL30/UL42, UmuD'2C, UmuD'2C-RecA-ATP, Vent polymerase, X family DANN polymerase, Y-family DNA polymerase eta

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.7 DNA-directed DNA polymerase

Activating Compound

Activating Compound on EC 2.7.7.7 - DNA-directed DNA polymerase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
optimal concentration of 20 mM, inhibition below and above 20 mM
2-mercaptoethanol
Aeropyrum pernix proliferating cell nuclear antigen 1
three genes encoding proliferating cell nuclear antigen (PCNA)-like sequences are identified in the genome of Aeropyrum pernix. The genes are cloned and expressed in Escherichia coli and the gene products are analyzed. All three proliferating cell nuclear antigen homologs stimulate the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II in Aeropyrum pernix to various extents. Aeropyrum pernix PCNA 3 (ApePCNA3) provides a most remarkable effect on both Pol I and Pol II
-
Aeropyrum pernix proliferating cell nuclear antigen 3
three genes encoding proliferating cell nuclear antigen (PCNA)-like sequences are identified in the genome of Aeropyrum pernix. The genes are cloned and expressed in Escherichia coli and the gene products are analyzed. All three proliferating cell nuclear antigen homologs stimulate the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II in Aeropyrum pernix to various extents. Aeropyrum pernix PCNA 3 (ApePCNA3) provides a most remarkable effect on both Pol I and Pol II
-
ATP
-
considerable enhancement in processivity at 1 mM ATP
Auxiliary subunits
-
pol III can repair short gaps created by nuclease in duplex DNA, for efficient replication of the long, single-stranded templates pol III requires auxiliary subunits beta, gamma and delta
-
bovine serum albumin
DNA polymerase is stabilized with an approximately 25% increase in enzyme activity
-
dimethyl sulfoxide
ethyleneglycol
-
5%, activity is stimulated by 30%
FEN1
-
stimulates strand displacement activity, DEN1 processes nicked DNA, thus removing a barrier to Pol lambda DNA synthesis. It results in a one-nucleotide gapped DNA molecule that is a favorite substrate of Pol lambda
-
glycerol
-
5%, activity is stimulated by 30%
Methanosarcina acetivorans proliferating cell nuclear antigen
stimulates translesion DNA synthesis
-
PCNA
-
stimulates strand displacement activity
-
Polyethylene glycol
Polymerase alpha accessory factors
-
pORF18
-
stimulates DNA polymerase activity of pORF30. pORF30 physically interacts with pORF18 through both a C-proximal region and the extreme C terminus
-
proliferating cell nuclear antigen
-
RecA
-
DNA polymerase V is activated by a RecA nucleoprotein filament, RecA transfers a single RecA-ATP stoichiometrically from its DNA 3'-end to free pol V (UmuD'2C) to form an active mutasome with the composition UmuD'2C-RecA-ATP
-
Replication factor A
-
Replication factor C
-
single-stranded binding protein
-
single-stranded-binding protein
-
SSB protein, Pol V has intrinsically weak DNA polymerase activity, but its catalytic activity can be stimulated in vitro in the presence the beta-processivity clamp, RecA protein bound to ssDNA, and single-stranded-binding protein
-
spermidine
SsoCdc6
-
Orc1/Cdc6 proteins stimulate the DNA-binding ability of SsoPolB1 and differentially regulate both its polymerase and nuclease activities
-
thiol
Triton X-100
DNA polymerase is stabilized with an approximately 25% increase in enzyme activity
additional information
-