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(2E)-2-(3-nitro-4-[[6-(trifluoromethyl)pyridin-3-yl]sulfanyl]benzylidene)-5-thioxo-1,3-thiazolidin-4-one
-
-
(2E)-2-(4-chloro-3-ethylbenzylidene)-5-thioxo-1,3-thiazolidin-4-one
-
-
(2E)-2-(pentafluorobenzylidene)-5-thioxodihydrothiophen-3(2H)-one
-
-
(2E)-2-[4-(2-hydroxyethyl)-3-nitrobenzylidene]-5-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-(4-chloro-3-nitrobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[3-bromo-4-[(4-fluorobenzyl)oxy]benzylidene]-1,3-thiazolidine-2,4-dione
-
-
(5Z)-5-[3-bromo-4-[(4-fluorophenyl)sulfanyl]benzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[3-nitro-4-(pyridin-3-ylsulfanyl)benzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-(4-methylphenoxy)-3-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-(cyclohexylsulfanyl)-3-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[(4-bromophenyl)sulfanyl]-3-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[(4-chlorophenyl)sulfanyl]-3-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[(4-fluorobenzyl)oxy]-3-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[(4-fluorobenzyl)oxy]benzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
(5Z)-5-[4-[(4-methylphenyl)sulfanyl]-3-nitrobenzylidene]-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-4-one
-
-
(9-adenylmethylcarbonyl)-4-aminobutyl triphosphate
-
fully competitive with respect to the nucleotide substrate dTTP, inhibits wild-type enzyme and mutant enzyme Y505A
(biphenylcarbonyl)-4-oxobutyl triphosphate
-
fully competitive with respect to the nucleotide substrate dTTP, inhibits wild-type enzyme and mutant enzyme Y505A; inhibits wild-type enzyme and mutant enzyme Y505A
(NH4)2SO4
-
optimal concentration of 20 mM, inhibition below and above 20 mM
1,2,3,4-tetrahydro-5-methoxynaphthalene-1,4-diol
-
i.e. nodulisporol. Strong inhibition of pol lambda, but does not influence the activities of mammalian pols alpha to kappa, and shows no effect on the activities of plant pols alpha and beta, prokaryotic pols, and other DNA metabolic enzymes such as calf terminal deoxynucleotidyl transferase, human immunodeficiency virus type-1 (HIV-1) reverse transcriptase, human telomerase, T7 RNA polymerase, and bovine deoxyribonuclease I
1,3-bis[2-chloroethyl]-2-nitrosourea
-
-
16-oxoaphidicholin
Tequatrovirus T4
-
-
2',3'-dideoxy-ATP
-
poor inhibitor
2',3'-dideoxyribosylthymine triphosphate
2',3'-dideoxythymidine 5'-triphosphate
2-(4-azidophenacyl)thio-2'-deoxyadenosine 5'-triphosphate
-
template-competitive DNA polymerase inhibitor
-
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
2-butylanilino-dATP
-
potent and highly selective inhibitor
2-methoxy-4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl 2,4-dichlorobenzoate
-
-
2-methoxy-4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl 3-bromobenzoate
-
-
2-thiomethyl-6-phenyl-4-(4'-hydroxybutyl)-1,2,4,-triazole (5,1-C)(1,2,4)triazine-7-one triphosphate
-
fully competitive with respect to the nucleotide substrate dTTP; fully competitive with respect to the nucleotide substrate dTTP, inhibits wild-type enzyme and mutant enzyme Y505A
3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
50% inhibition at 0.22 mM
3'-azido-2',3'-dideoxythymidine triphosphate
-
-
3,4-dihydro-4-hydroxy-8-methoxynaphthalen-1(2H)-one
-
i.e. nodulisporone. Strong inhibition of pol lambda, but does not influence the activities of mammalian pols alpha to kappa, and shows no effect on the activities of plant pols alpha and beta, prokaryotic pols, and other DNA metabolic enzymes such as calf terminal deoxynucleotidyl transferase, human immunodeficiency virus type-1 (HIV-1) reverse transcriptase, human telomerase, T7 RNA polymerase, and bovine deoxyribonuclease I
3,5-dimethyl-8-methoxy-3,4-dihydroisocoumarin
-
-
3-(2'-deoxy-beta-D-erythro-pentofuranosyl) pyrimido[1,2-alpha]purin-10(3H)-one
-
i.e. M1dG. When paired opposite cytosine in duplex DNA at physiological pH,M1dG undergoes ring opening to form N2-(3-oxo-1-propenyl)-dG. To improve the understanding of the basis for M1dG-induced mutagenesis, the mechanism of translesion DNA synthesis opposite M1dG by the model Y-family polymerase Dpo4 is studied at a molecular level using kinetic and structural approaches. Steady-state and transient-state kinetic results both indicate that Dpo4 catalysis is inhibited by M1dG (260-2900-fold), with dATP being the favored insertion event for both sequences tested
3-epiaphidicholine
Tequatrovirus T4
-
-
35dd(G) DNA
-
competitive inhibition
-
4-chloromercuribenzoic acid
4-chlorophenyl 2,4-dinitrophenyl sulfide
-
-
4-hydroxy-5-methyl-3-tetradecyl-dihydrofuran-2(3H)-one
-
-
4-hydroxymercuribenzoate
Tequatrovirus T4
-
95% inhibition at 0.4 mM
4-oxo-dihydroquinoline
-
binds at the polymerase active site interacting non-covalently with both the polymerase and the DNA duplex
4-[(4-methylphenyl)sulfanyl]-3-nitrobenzaldehyde
-
-
4-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]-2-methoxyphenyl 3-bromobenzoate
-
-
4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl 2-chlorobenzoate
-
-
5-(5-nitro-2-[[6-(trifluoromethyl)pyridin-3-yl]sulfanyl]benzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
-
5-[2-(cyclohexylsulfanyl)-5-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
5-[2-[(4-chlorophenyl)sulfanyl]-5-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
5-[2-[(4-methylphenyl)sulfanyl]-5-nitrobenzylidene]-2-thioxo-1,3-thiazolidin-4-one
-
-
6-chloro-1,4-dihydro-4-oxo-1-(beta-D-ribofuranosyl) quinoline-3-carboxylic acid
-
noncompetitive inhibitor
6-chloro-1,4-dihydro-4-oxo-1H-quinoline-3-carboxylic acid
-
-
acrolein
-
irreversible inhibition
acyclovir triphosphate
-
-
alpha-(4-azidophenyl)-1,N6-etheno-dATP
-
template-competitive DNA polymerase inhibitor
alpha-Amanitin
Ruellia sp.
-
88% inhibition at 0.2 mg/ml
ammonium-21-tungsto-9-antimoniate
-
HPA-23, antiviral drug, noncompetitive to TTP, activated DNA, poly(rA)-oligo(dT), wheat germ DNA polymerase A, a gamma-like DNA polymerase
-
aptamer
-
nucleotide ligands of small size and unique three-dimensional structure, TQ21 family, presence of loop is necessary for inhibition
-
Arabinofuranosylnucleoside triphosphates
-
and related compounds
aurintricarboxylic acid
-
potent nanomolar inhibitor of pol beta, pol iota, and pol zeta
Benzyloxycarbonyl-Leu-Leu-al
cerebroside sulfate ester
-
inhibition of polymerase alpha and gamma
cholesterol hemisuccinate
cis-syn thymine dimer
Dpo4 is severely blocked by the presence of a cis-syn thymine dimer in the template
cloretazine
-
i.e. 1,2-bis[methylsulfonyl]-1-[2-chloroethyl]-2-[(methylamino)carbonyl]hydrazine
corylifolin
Betapolyomavirus macacae
-
isolated from Psoralea corylifolia
coumermycin A
-
50% inhibition at 0.05 mM
daidzein
Betapolyomavirus macacae
-
isolated from Psoralea corylifolia, slight inhibition
dATP
-
using dATP as an inhibitor and dCTP as a varied substrate, the pattern of inhibition is competitive
deoxynucleoside triphosphate
-
work as competitive inhibitors depending on their incorporation sites, A-incorporation is strongly inhibited by dTTP and dGTP, T-incorporation is inhibited by dATP, G-and C-incorporation sites are less sensitive to competitive inhibition
Dideoxyadenosine triphosphate
-
weak inhibitor
dideoxyguanosine triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
Dideoxythymidine triphosphate
ellagic acid
-
potent nanomolar inhibitor of pol beta, pol iota, and pol zeta
epigallocatechin gallate
-
-
flavonoids
Ruellia sp.
-
substitution of hydroxyl groups with glycosides
gemcitabine
-
i.e. 2'-deoxy-2',2'-difluorocytidine, when gemcitabine is encountered as a template base DNA polymerase gamma pauses at the lesion and one downstream position but eventually elongates the primer to full-length product, these pauses are because of a 1000fold decrease in nucleotide incorporation efficiency
glycolipids
-
sulfate- and sialic acid-containing
-
hydroxyphenylazouracil
-
inhibits only polymerase III
hymenoic acid
-
specific inhibitor of human DNA polymerase lambda, non-competitive inhibition with respect to both the DNA template-primer and the dNTP, i.e trans-4-[(1'E,5'S)-5'-carboxy-1'-methyl-1'-hexenyl]cyclohexanecarboxylic acid, does not influence the activities of the other mammalian pols alpha, gamma, delta, epsilon, eta, iota, and kappa, and also shows no effect even on the activity of pol beta
hypoxanthine
-
traces of uracil and hypoxanthine in DNA can lead to inhibition of the polymerase chain reaction by archaeal DNA polymerases
iodoacetate
-
DNA polymerase beta
N-(2,4-dinitro-5-fluorophenyl)-2-aminoethyl triphosphate
-
inhibition of mutant enzyme Y505A, inactive against wild-type enzyme
N-(2,4-dinitro-5-fluorophenyl)-4-aminobutyl triphosphate
-
fully competitive with respect to the nucleotide substrate dTTP, inhibits wild-type enzyme and mutant enzyme Y505A
N-(2,4-dinitro-5-imidazolylphenyl)-2-aminoethyl triphosphate
-
inhibition of mutant enzyme Y505A, inactive against wild-type enzyme
N-(2,4-dinitro-5-imidazolylphenyl)-4-aminobutyl triphosphate
-
inhibition of mutant enzyme Y505A, inactive against wild-type enzyme
N-(2,4-dinitrophenyl)-4-aminobutyl triphosphate
-
fully competitive with respect to the nucleotide substrate dTTP, inhibits wild-type enzyme and mutant enzyme Y505A
N-(benzyloxycarbonyl)-4-aminobutyl triphosphate
-
fully competitive with respect to the nucleotide substrate dTTP, inhibits wild-type enzyme and mutant enzyme Y505A; inhibits wild-type enzyme and mutant enzyme Y505A
N-[6-N-(2,4-dinitrophenyl)aminohexanoyl]-2-aminoethyl triphosphate
-
inhibition of mutant enzyme Y505A, inactive against wild-type enzyme
N2,N2-dimethyl guanine
the presence of N2,N2-dimethyl guanine lowers the catalytic efficiency for incorporation of dCTP of DNA polymerase Dpo4 16000fold
N2,N2-dimethylguanine
the presence of N2,N2-dimethylguanine lowers the catalytic efficiency of the DNA polymerase Dpo4 16000fold. Dpo4 inserts dNTPs almost at random during bypass of N2,N2-dimethylguanine, and much of the enzyme is kinetically trapped by an inactive ternary complex when N2,N2-dimethylguanine is present
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
N2-CH2(6-benzo[a]pyrenyl)guanine
-
severely blocks activity
N2-CH2(9-anthracenyl)guanine
-
severely blocks activity, frequencies of dATP misinsertion and extension beyond mispairs are proportionally increased, great decrease in pre-steady-state kinetic burst rate. 2.6fold decrease in dCTP binding affinity and increased DNA substrate binding affinity
neobavaisoflavone
Betapolyomavirus macacae
-
isolated from Psoralea corylifolia, inhibition at moderate to high concentrations
NSC-666715
potent, small molecular weight inhibitor of DNA polymerase beta, blocks Pol-beta-directed single-nucleotide- and long-patch-base excisison repair
pamoic acid
-
pol beta inhibitor
Phosphoformate
Herpes simplex virus
-
-
pyridoxal 5'-phosphate
-
50% inhibition at 0.48 mM
RecA
-
exonuclease activity of pol II can be inhibited by the presence of RecA protein and single-strand binding protein
-
Replication factor C
DNA polymerization by Dpo2 and Dpo3 is strongly inhibited; DNA polymerization by Dpo2 and Dpo3 is strongly inhibited
-
resveratrol
Betapolyomavirus macacae
-
isolated from Psoralea corylifolia
rhodanines
-
most potent inhibitors for DNA Pol lambda, they are up to 10times less active against the highly similar DNA polymerase beta, structure-activity relationships, overview. 5-Arylidene-2,4-thiazolidinediones are class I rhodanines, rhodanine class II has members of carbohydrazides, and class III contains a common 2,4-pentadione substructure element
-
single-stranded binding protein
DNA polymerization by Dpo2 and Dpo3 is strongly inhibited; DNA polymerization by Dpo2 and Dpo3 is strongly inhibited; DNA synthesis by DNA polymerase Dpo2 and Dpo3 is remarkably decreased by single-stranded binding protein; DNA synthesis by DNA polymerase Dpo2 and Dpo3 is remarkably decreased by single-stranded binding protein
-
Sodium dodecyl sulfate
-
-
sphingosine
-
polymerase alpha and beta
SsoCdc6-2 protein
-
inhibits both the DNA-binding activity and DNA polymerization activity of SsoPolY on the DNA substrates containing mismatched bases, while it forms a large complex with SsoPolY and stimulates DNA-binding activity on paired primer-template DNA substrates
-
stavudine triphosphate
-
-
sucrose
-
50% inhibition with 4% sucrose
sulfoglycolipid beta-sulfoquinovosyldiacylglycerol
-
inhibits the DNA polymerase activity of pol lambda by binding to the Met1-Arg95 region and inhibiting its nuclear transit
-
sulfoquinovosyl diacylglycerol
-
significant inhibition of activity at 0.1 mg/ml in strain JM109, but not in strains TOP10, TOP10F, or DH5alpha
Uracil
-
traces of uracil and hypoxanthine in DNA can lead to inhibition of the polymerase chain reaction by archaeal DNA polymerases
vitamin K3
-
more than 80% inhibition of pol gamma at 0.03 mM, does not affect other human DNA polymerase isozymes
zalcitabine triphosphate
-
-
Zn2+
Herpes simplex virus
-
-
[3-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenoxy]acetic acid
-
-
1,10-phenanthroline
-
-
1-deoxyrubralactone
-
potent inhibitor of isozymes pol kappa, pol lambda, pol iota, and pol eta, but does not inhibit DNA polymerase isozymes pol delta, pol epsilon, and pol gamma
1-deoxyrubralactone
-
potent inhibitor
10-epi-pyragonicin
-
-
19-epi-jimenezin
-
-
2',3'-dideoxyribosylthymine triphosphate
-
17% and 33% inhibition are observed with ddTTP/dTTP ratios of 1:1 and 5:1, respectively
2',3'-dideoxyribosylthymine triphosphate
-
the enzyme is slightly sensitive to. 25% inhibition is observed with a ddTTP/dTTP ratio of 50
2',3'-dideoxythymidine 5'-triphosphate
-
-
2',3'-dideoxythymidine 5'-triphosphate
-
-
2',3'-dideoxythymidine 5'-triphosphate
Ruellia sp.
-
-
2',3'-dideoxythymidine 5'-triphosphate
-
-
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
-
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
DNA polymerase alpha
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold lower concentration than DNA polymerase delta
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
polymerase delta and epsilon
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
DNA polymerase alpha
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
DNA polymerase alpha
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
DNA polymerase alpha
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold lower concentration than DNA polymerase delta
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
DNA polymerase alpha
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold lower concentration than DNA polymerase delta
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold lower concentration than DNA polymerase delta
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold lower concentration than DNA polymerase delta
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
Tequatrovirus T4
-
phage T4 enzyme inhibited with lower sensitivity than other members of the B family DNA polymerases
2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
DNA polymerase alpha
4-chloromercuribenzoic acid
-
-
4-chloromercuribenzoic acid
-
-
4-chloromercuribenzoic acid
-
-
adefovir diphosphate
-
-
Allicin
-
-
alliin
-
-
aphidicolin
-
10% inhibition at 0.05 mM
aphidicolin
-
DNA polymerase epsilon, 80% inhibition at 0.002 mg/ml
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
polymerase alpha and delta
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
DNA polymerase alpha; polymerase alpha, delta and epsilon
aphidicolin
-
relatively insensitive
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
inhibition at 0.01 and 0.1 mg/ml
aphidicolin
-
IC50: 0.01 mM
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
Herpes simplex virus
-
-
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
DNA polymerase alpha
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
inhibition is competitive for dCTP, noncompetitive for dATP, dGTP and dTTP and uncompetitive for activated DNA
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
alpha-like enzyme
aphidicolin
Ruellia sp.
-
-
aphidicolin
-
50% inhibition at 0.0006 mM
aphidicolin
Tequatrovirus T4
-
phage T4 enzyme inhibited with lower sensitivity than other members of the B family DNA polymerases
aphidicolin
-
DNA polymerase alpha; DNA polymerase delta; DNA polymerase epsilon
aphidicolin
-
50% inhibition at 0.05 mM
ara-ATP
Herpes simplex virus
-
-
Ara-CTP
-
no inhibition of pol I and III
Ara-CTP
-
polymerase II and III
Ara-CTP
-
50% inhibition at 0.05 mM
bakuchiol
Betapolyomavirus macacae
-
isolated from Psoralea corylifolia
bakuchiol
-
inhibition of polymerase epsilon
Benzyloxycarbonyl-Leu-Leu-al
-
-
Benzyloxycarbonyl-Leu-Leu-al
-
-
Carbonyldiphosphonate
-
DNA polymerase delta; no inhibition of polymerase alpha
Carbonyldiphosphonate
-
polymerase delta and epsilon
Carbonyldiphosphonate
-
polymerase alpha, delta and epsilon
Carbonyldiphosphonate
-
DNA polymerase delta; no inhibition of polymerase alpha
Carbonyldiphosphonate
-
DNA polymerase delta; no inhibition of polymerase alpha
Carbonyldiphosphonate
-
DNA polymerase delta; no inhibition of polymerase alpha
Carbonyldiphosphonate
-
-
Carbonyldiphosphonate
-
50% inhibition at 0.15 mM
Carbonyldiphosphonate
-
DNA polymerase delta; no inhibition of polymerase alpha
Carbonyldiphosphonate
-
-
Carbonyldiphosphonate
-
-
Carbonyldiphosphonate
-
-
Carbonyldiphosphonate
Tequatrovirus T4
-
-
Carbonyldiphosphonate
-
DNA polymerase delta; no inhibition of polymerase alpha
cholesterol hemisuccinate
-
pol beta
cholesterol hemisuccinate
-
pol beta; pol iota; pol kappa; pol lambda
cholesterol hemisuccinate
-
pol beta
Dansyl-Leu-Leu-Leu-CH2Cl
-
inhibition of pol I
Dansyl-Leu-Leu-Leu-CH2Cl
-
inhibition of polymerase alpha, beta and gamma
ddTTP
0.4 mM, about 80% loss of activity
ddTTP
-
processivity is strongly inhibited in the presence of 0.01 and 0.02 mM ddTTP
diallyl pentasulfide
-
-
diallyl tetrasulfide
-
-
diallyl trisulfide
-
-
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
DNA polymerase beta
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
polymerase epsilon, slightly sensitive
Dideoxynucleoside 5'-triphosphate
-
DNA polymerase beta
Dideoxynucleoside 5'-triphosphate
-
DNA polymerase beta
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
Herpes simplex virus
-
-
Dideoxynucleoside 5'-triphosphate
-
DNA polymerase beta
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
polymerase epsilon, slightly sensitive
Dideoxynucleoside 5'-triphosphate
-
DNA polymerase beta
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
polymerase epsilon, slightly sensitive
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
polymerase epsilon, slightly sensitive
Dideoxynucleoside 5'-triphosphate
-
polymerase epsilon, slightly sensitive
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
DNA polymerase beta
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxynucleoside 5'-triphosphate
-
-
Dideoxythymidine triphosphate
-
inhibition at 0.05 mM
Dideoxythymidine triphosphate
-
-
Dideoxythymidine triphosphate
-
75% inhibition at 0.000001 mM
Dideoxythymidine triphosphate
Ruellia sp.
-
-
Dideoxythymidine triphosphate
-
-
Dideoxythymidine triphosphate
-
50% inhibition at 0.25 mM
dimethyl sulfoxide
-
inhibition of DNA polymerase epsilon, stimulation of DNA polymerase delta
dimethyl sulfoxide
-
stimulates DNA polymerase alpha and delta, inhibits human DNA polymerase epsilon
dimethyl sulfoxide
-
67% inhibition of RNAse H activity at 10%
diphosphate
-
DNA polymerase beta
diphosphate
Tequatrovirus T4
-
analogs, phage T4 enzyme inhibited with lower sensitivity than other members of the B family DNA polymerases
EDTA
-
-
EDTA
-
complete inhibition
EDTA
-
complete inhibition
EDTA
2 mM, complete inhibition
EDTA
Mg2+ and Mn2+, can restore activity
ethanol
-
3%, 50% inhibition
ethanol
Ruellia sp.
-
21% inhibition at 10%
glycerol
-
50% inhibition with 15% glycerol
glycerol
-
33% inhibition of RNAse H activity at 10%
Halenaquinol sulfate
-
potential inhibitor of DNA polymerase alpha and epsilon, less effective against Escherichia coli DNA polymerase
Halenaquinol sulfate
-
potential inhibitor of DNA polymerase alpha and epsilon, less effective against Escherichia coli DNA polymerase
jimenezin
-
-
K+
-
optimum concentration 125 mM, inhibition at higher concentration
K+
-
50% inhibition at 270 mM
K+
-
optimum concentration 50 mM, inhibition at higher concentration
KCl
at 100 mM KCl, 98%b loss of activity
KCl
150 mM, 90% inhibition
KCl
-
inhibition at 0.2 mM
KCl
inhibition above 20 mM
KCl
100 mM, 80% inhibition
lamivudine triphosphate
-
-
lamivudine triphosphate
-
-
lysophosphatidic acid
-
isolated from myxamoebae of Physarum polycephalum, 90% inhibition of polymerase alpha, weak inhibitor of polymerase beta
lysophosphatidic acid
-
weak inhibitor
Mg2+
-
DNA polymerase alpha: free Mg2+ competes with primer for enzyme binding, dramatic inhibition at Mg2+ concentration above the optimum
Mg2+
-
DNA polymerase alpha: free Mg2+ competes with primer for enzyme binding, dramatic inhibition at Mg2+ concentration above the optimum
Mg2+
-
required, substrate-like inhibition by Mg2+ occur, the inhibition is not due to enzyme inactivation, but instead due to the decrease in rate of a step after chemistry
Mg2+
-
Mg2+ concentrations beyond 6 mM are inhibitory in activity assay
Mn2+
-
inhibits 3'-5'-proofreading activity, thereby decreasing the fidelity of DNA replication by 50%
mucocin
-
-
muconin
-
-
N-ethylmaleimide
-
polymerase I and II; polymerase III
N-ethylmaleimide
-
DNA polymerase alpha, gamma, delta
N-ethylmaleimide
-
polymerase alpha and delta
N-ethylmaleimide
-
inhibition at 1 mM
N-ethylmaleimide
-
relatively insensitive
N-ethylmaleimide
-
DNA polymerase alpha, gamma, delta
N-ethylmaleimide
-
abolishes polymerase III activity at 10 mM
N-ethylmaleimide
-
great sensitivity of phage-induced enzyme, relative insensitivity of pol I
N-ethylmaleimide
-
DNA polymerase alpha, gamma, delta
N-ethylmaleimide
-
polymerase gamma
N-ethylmaleimide
Herpes simplex virus
-
-
N-ethylmaleimide
-
DNA polymerase alpha, gamma, delta
N-ethylmaleimide
-
DNA polymerase
N-ethylmaleimide
-
60% inhibition at 0.2 mM
N-ethylmaleimide
-
69-96% inhibition at 0.0007 mM, 46-54% inhibition at 0.007 mM
N-ethylmaleimide
-
50% inhibition at less than 1 mM
N-ethylmaleimide
-
DNA polymerase alpha, gamma, delta
N-ethylmaleimide
1 mM, about 80% loss of activity
N-ethylmaleimide
Ruellia sp.
-
59% inhibition at 1 mM
N-ethylmaleimide
-
5 mM; 66% inhibition
N-ethylmaleimide
Tequatrovirus T4
-
great sensitivity of phage-induced enzyme, relative insensitivity of pol I
N-ethylmaleimide
Tequintavirus T5
-
great sensitivity of phage-induced enzyme, relative insensitivity of pol I
N-ethylmaleimide
-
inhibited when preincubation with this reagent is performed at 65°C
N-ethylmaleimide
-
DNA polymerase alpha, gamma, delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
-
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold; lower concentration than DNA polymerase delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
100-fold, polymerase delta and epsilon
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition at 0.1 mM
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold; lower concentration than DNA polymerase delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold; lower concentration than DNA polymerase delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold; lower concentration than DNA polymerase delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
inhibition of DNA polymerase alpha at 100fold; lower concentration than DNA polymerase delta
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
alpha-like enzyme relatively resistant
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
-
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
-
50% inhibition at 0.08 mg/ml
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
Tequatrovirus T4
-
mechanism depends upon assay conditions, reversible competitive inhibition predominates
NaCl
-
-
NaCl
in absence of NaCl, Tga PolB displays 67% polymerization activity. From 50 to 200 mM NaCl, the activity is higher than 90%, but it is significantly reduced at NaCl concentrations above 400 mM
NaCl
-
enzyme is inhibited 50% by 200 mM
NH4Cl
-
-
NH4Cl
-
150 mM, 50% inhibition
Oosporein
-
50% inhibition at 0.7 mM
Oosporein
Herpes simplex virus
-
50% inhibition at 0.075 mM
Oosporein
-
50% inhibition at 0.61 mM
phosphate
-
strong inhibitor
phosphate
-
DNA polymerase beta
Phosphonoacetate
-
-
Phosphonoacetate
Herpes simplex virus
-
-
poly(rA)-p(dT)45
-
inhibitory effect towards the reverse transcriptase activity of K4polL329A
-
poly(rA)-p(dT)45
inhibitory effect towards the reverse transcriptase activity of M1pol
-
pyragonicin
-
-
pyranicin
-
potent inhibitor
pyranicin
-
potent inhibitor
pyranicin
-
potent inhibitor
pyranicin
-
potent inhibitor
pyranicin
Tequatrovirus T4
-
-
Salt
-
concentrations above 50 mM inhibit: human KB cell polymerase alpha
Salt
-
sensitive to high ionic strength
Salt
Tequatrovirus T4
-
optimal activity in presence of total salt concentration of approximately 0.1 M, 97% inhibition at 0.3 M
Salt
-
sensitive to high ionic strength
SH-blocking agents
-
pol II and III
-
SH-blocking agents
-
pol II and III
-
SH-blocking agents
-
pol II and III
-
SH-blocking agents
-
pol II and III
-
single-stranded DNA
-
-
single-stranded DNA
-
inhibition of polymerase alpha, competitive with respect to activated DNA substrate
single-stranded DNA
-
inhibition of polymerase alpha, competitive with respect to activated DNA substrate
talaroflavone
-
-
tenofovir diphosphate
-
-
tenofovir diphosphate
-
-
zidovudine triphosphate
-
-
zidovudine triphosphate
-
-
additional information
-
Pol X is not inhibited noticeably by up to 1 mM diphosphate, at higher concentrations there is modest inhibition
-
additional information
-
-
-
additional information
-
no inhibition of polymerase I by ara-CTP
-
additional information
-
-
-
additional information
-
no inhibition of DNA polymerase epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate
-
additional information
-
no inhibition by dideoxynucleoside 5'-triphosphate; no inhibition of DNA polymerase beta and gamma by aphidicolin; no inhibition of DNA polymerase beta by N-ethylmaleimide; no inhibition of DNA polymerase beta, gamma, delta, epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate; no inhibition of polymerase alpha and delta by dideoxynucleoside 5'-triphosphate
-
additional information
-
inhibitor analysis of calf thymus DNA polymerase alpha, delta and epsilon
-
additional information
-
-
-
additional information
-
not inhibited by 1-deoxyrubralactone and talaroflavone
-
additional information
-
DNA polymerase alpha is not inhibited by vitamin K1, vitamin K2, and vitamin K3
-
additional information
-
DNA polymerase alpha is not inhibited by allicin, alliin, diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
-
not inhibited by gamma-lactone
-
additional information
-
not inhibited by 1-deoxyrubralactone and talaroflavone
-
additional information
-
DNA polymerase isozymes I and II are not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
-
no inhibition by aphidicolin; no inhibition by ara-CTP
-
additional information
-
no inhibition by dideoxynucleoside 5'-triphosphate; no inhibition of DNA polymerase beta and gamma by aphidicolin; no inhibition of DNA polymerase beta by N-ethylmaleimide; no inhibition of DNA polymerase beta, gamma, delta, epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate; no inhibition of polymerase alpha and delta by dideoxynucleoside 5'-triphosphate
-
additional information
-
enzyme is resistant to NEM and 2',3'-dideoxythymidine 5-triphosphate
-
additional information
-
no inhibition by entecavir
-
additional information
-
DNA polymerase isozymes alpha, delta and epsilon are not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
-
pol II and III not inhibited by pol I antiserum
-
additional information
-
no inhibition of DNA polymerase beta or gamma from various eukaryotic species, DNA polymerase I from E. coli by lysophosphatidic acid
-
additional information
-
not inhibited by 1-deoxyrubralactone and talaroflavone
-
additional information
-
DNA polymerase I is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
-
monogalactosyl diacylglycerol and digalactosyl diacylglycerol do not have any inhibitory effect on enzymatic activity
-
additional information
-
no inhibition by dideoxynucleoside 5'-triphosphate; no inhibition of DNA polymerase beta and gamma by aphidicolin; no inhibition of DNA polymerase beta by N-ethylmaleimide; no inhibition of DNA polymerase beta, gamma, delta, epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate; no inhibition of polymerase alpha and delta by dideoxynucleoside 5'-triphosphate
-
additional information
polI activity requires the presence of monovalent ions, above 100 mM, monovalent salts become inhibitory for the activity
-
additional information
-
no inhibition by dideoxynucleoside 5'-triphosphate; no inhibition of DNA polymerase beta and gamma by aphidicolin; no inhibition of DNA polymerase beta by N-ethylmaleimide; no inhibition of DNA polymerase beta, gamma, delta, epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate; no inhibition of polymerase alpha and delta by dideoxynucleoside 5'-triphosphate
-
additional information
-
-
-
additional information
-
no inhibition by (9-adenylmethylcarbonyl)-4-aminobutyl, N-(2,4-dinitrophenyl)-4-aminobutyl triphosphate, N-(2,4-dinitro-5-fluorophenyl)-4-aminobutyl triphosphate, N-[6-N-(2,4-dinitrophenyl)aminohexanoyl]-2-aminoethyl triphosphate, N-(2,4-dinitro-5-fluorophenyl)-2-aminoethyl triphosphate, N-(2,4-dinitro-5-imidazolylphenyl)-4-aminobutyl triphosphate and N-(2,4-dinitro-5-imidazolylphenyl)-2-aminoethyl triphosphate
-
additional information
-
DNA polymerase isozymes are not inhibited by vitamin K1 and vitamin K2
-
additional information
-
DNA polymerase lambda is strongly inhibited by an extract from Allium sativum consisting of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide, competitive inhibitor
-
additional information
-
isozyme DNA polymerase lambda is not inhibited by mucocin
-
additional information
-
no inhibition by 2',3'-dideoxythymidine 5'-triphosphate; no inhibition by aphidicolin; no inhibition by ara-CTP
-
additional information
-
no inhibition by dideoxynucleoside 5'-triphosphate; no inhibition of DNA polymerase beta and gamma by aphidicolin; no inhibition of DNA polymerase beta by N-ethylmaleimide; no inhibition of DNA polymerase beta, gamma, delta, epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate; no inhibition of polymerase alpha and delta by dideoxynucleoside 5'-triphosphate
-
additional information
-
no inhibition of DNA polymerase beta or gamma from various eukaryotic species, DNA polymerase I from E. coli by lysophosphatidic acid
-
additional information
-
-
-
additional information
-
DNA polymerase delta is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
no inhibition by 0.1 mM aphidicolin
-
additional information
no inhibition by 0.1 mM aphidicolin
-
additional information
no inhibition by aphidicolin (4 mM)
-
additional information
DNA polymerizing activity is not inhibited by aphidicolin at concentrations up to 2 mM that inhibit the activities of Pfu Pol I and other alpha-like DNA polymerases.
-
additional information
-
DNA polymerizing activity is not inhibited by aphidicolin at concentrations up to 2 mM that inhibit the activities of Pfu Pol I and other alpha-like DNA polymerases.
-
additional information
-
DNA polymerase beta is not inhibited by vitamin K1, vitamin K2, and vitamin K3
-
additional information
-
DNA polymerase beta is strongly inhibited by an extract from Allium sativum consisting of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide, competitive inhibitor
-
additional information
-
not inhibited by gamma-lactone, mucocin, jimenezin, and 19-epi-jimenezin
-
additional information
-
reverse gyrase inhibits DNA polymerase PolB1. Inhibition of PolY activity depends on both ATPase and topoisomerase activities of reverse gyrase, suggesting that the intact positive supercoiling activity is required for PolY inhibition. In vivo, reverse gyrase and PolY are degraded after induction of DNA damage. Inhibition by reverse gyrase and degradation might act as a double mechanism to control DNA polymerase PolY and prevent its potentially mutagenic activity when undesired. Inhibition of a translesion polymerase by topoisomerase-induced modification of DNA structure may represent a mechanism of regulation of these enzymes
-
additional information
-
the enzyme is resistant to 0.02 mg/ml aphidicolin
-
additional information
no inhibition by 0.02 mg/ml aphidicolin. The enzyme is inhibited by dideoxy- and arabinosine-analogs and SH-blocking agents
-
additional information
Tequatrovirus T4
-
inhibitor analysis of bacteriophage T4 DNA polymerase
-
additional information
Tequatrovirus T4
-
not inhibited by 1-deoxyrubralactone and talaroflavone
-
additional information
Tequatrovirus T4
-
DNA polymerase is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
PI-TfuII is inhibited by one of the cleavage products
-
additional information
-
the enzyme is resistant to aphidicolin
-
additional information
-
high concentrations of DNA-primed RNA template decrease the efficiency of cDNA synthesis with bacterial family A DNA polymerases
-
additional information
-
not inhibited by 1-deoxyrubralactone and talaroflavone
-
additional information
-
DNA polymerase is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide
-
additional information
high concentrations of DNA-primed RNA template decrease the efficiency of cDNA synthesis with bacterial family A DNA polymerases
-
additional information
-
enzyme is not inhibited by cytosine-beta-D-arabinofuranoside 5'-triphosphate which is an inhibitor of alpha-polymerase, monoclonal antibodies against human DNA polymerase alpha do not bind; no inhibition by aphidicolin
-
additional information
-
no inhibition by dideoxynucleoside 5'-triphosphate; no inhibition of DNA polymerase beta and gamma by aphidicolin; no inhibition of DNA polymerase beta by N-ethylmaleimide; no inhibition of DNA polymerase beta, gamma, delta, epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate; no inhibition of polymerase alpha and delta by dideoxynucleoside 5'-triphosphate
-