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20.14 - 56.7
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
690
5-ethynyl-dCTP
-
pH and temperature not specified in the publication
-
730
5-ethynyl-dUTP
-
pH and temperature not specified in the publication
-
0.016 - 25.38
5-Methyl-dCTP
1250
5-phenyl-dCTP
-
pH and temperature not specified in the publication
-
1180
5-phenyl-dUTP
-
pH and temperature not specified in the publication
-
1100
5-vinyl-dCTP
-
pH and temperature not specified in the publication
-
1350
5-vinyl-dUTP
-
pH and temperature not specified in the publication
-
0.2 - 240.9
7-deaza-2'-deoxyadenosine 5'-triphosphate
970
7-deaza-dGTP
-
pH and temperature not specified in the publication
880
7-ethynyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
2330
7-ethynyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
900
7-methyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
1140
7-methyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
2410
7-phenyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
3340
7-phenyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
650
7-vinyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
1450
7-vinyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
186
Cy3-dATP
-
pH 8.0, 25°C
-
583
Cy3-dCTP
-
pH 8.0, 25°C
-
146
Cy3-dGTP
-
pH 8.0, 25°C
-
176
Cy3-dUTP
-
pH 8.0, 25°C
-
0.31
dPTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.0000000019 - 18800
dTTP
0.04 - 0.625
N1-methyl-2'-deoxyadenosine 5'-triphosphate
9.2 - 11.7
North-methanocarba-dATP
6.8
South-methanocarba-dATP
pH 7.5, 37°C
2060
TTP
-
pH and temperature not specified in the publication
additional information
additional information
-
20.14
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
56.7
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.03
2-thio-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.6
2-thio-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.016
5-Methyl-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
25.38
5-Methyl-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.2
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.29
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite an abasic site
71.4
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
168.7
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
240.9
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.000000041
dATP
pH 7.5, 37°C, DNA polymerase Dpo3
0.00000086
dATP
pH 7.5, 37°C, DNA polymerase Dpo1
0.00011
dATP
pH 7.5, 37°C, DNA polymerase Dpo4
0.00046
dATP
pH 7.8, 37°C, incorporation of dATP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.001
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0011
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0011
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0016
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0021
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0022
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0026
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0032
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.0034
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0043
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.0058
dATP
-
template base: dC, pH 7.5, 37°C
0.01
dATP
pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.01
dATP
pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5'-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3'
0.012
dATP
-
template base: dG, pH 7.5, 37°C
0.02
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.026
dATP
-
template base: dA, pH 7.5, 37°C
0.028
dATP
-
template base: dX, pH 7.5, 37°C
0.03
dATP
pH 7.8, 37°C, dATP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.03
dATP
pH 7.8, 37°C, dATP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-TCAATCCTTACGAGCATCGCCCCC-3'
0.06
dATP
-
template base: dI, pH 7.5, 37°C
0.099
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.125
dATP
pH 7.8, 37°C, incorporation of dATP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8ocoG)CACT-5'
0.138
dATP
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.31
dATP
pH 7.5, 60°C, incorporation opposite an abasic site
0.731
dATP
-
pH 8.0, 37°C, recombinant enzyme
1
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410V
1.46
dATP
-
pH 8.0, 50°C, recombinant enzyme
2
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409I
2
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410I
3
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409V
4
dATP
-
55°C, pH not specified in the publication, mutant enzyme A408S
4
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410L
9
dATP
-
55°C, pH not specified in the publication, wild-type enzyme
14
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409M
33
dATP
-
template base: dT, pH 7.5, 37°C
44
dATP
-
pH 9.0, 37°C, recombinant mutant enzyme
75
dATP
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
235.7
dATP
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
287.5
dATP
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
990
dATP
-
pH and temperature not specified in the publication
6004
dATP
-
pH 8.0, 25°C
0.0000054
dCTP
pH 7.5, 37°C, DNA polymerase Dpo2
0.0000071
dCTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.000042
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0025
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.0054
dCTP
-
template base: dA, pH 7.5, 37°C
0.0061
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0061
dCTP
-
template base: dC, pH 7.5, 37°C
0.0095
dCTP
-
template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C
0.015
dCTP
-
template base: dX , pH 7.5, 37°C
0.016
dCTP
-
template base: dT, pH 7.5, 37°C
0.019
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.021
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.026
dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.027
dCTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.083
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.11
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.12
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.31
dCTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.7
dCTP
pH 7.8, 37°C, dCTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-TCAATCCTTACGAGCATCGCCCCC-3'
1.2
dCTP
pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5'-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3'
1.7
dCTP
pH 7.8, 37°C, incorporation of dCTP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
1.9
dCTP
pH 7.5, 37°C, dCTP insertion opposite the N6dA-(OH)2butyl-GSH in 5'-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3'
2.3
dCTP
pH 7.5, 37°C, dCTP insertion opposite the N6dA-butanetriol in 5'-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3'
2.4
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
2.5
dCTP
pH 7.5, 37°C, dCTP insertion opposite an unmodified deoxyadenosine in 5'-TCTCAGTTTATGGACCACC-3'
2.8
dCTP
pH 7.8, 37°C, dCTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
3.9
dCTP
pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
6.5
dCTP
-
template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C
11
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
11.36
dCTP
pH 7.8, 37°C, incorporation of dCTP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8ocoG)CACT-5'
23
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
28
dCTP
37°C, pH 7.4, mutant enzyme T239W
28
dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
31
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
34
dCTP
37°C, pH 7.4, mutant enzyme N188W
34
dCTP
-
template base: dI, pH 7.5, 37°C
37
dCTP
-
pH 9.0, 37°C, recombinant mutant enzyme
370
dCTP
-
template base: dG, pH 7.5, 37°C
910
dCTP
-
pH and temperature not specified in the publication
6480
dCTP
-
pH 8.0, 25°C
0.000000068
dGTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.0000027
dGTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.00032
dGTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.0004
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.00064
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0029
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0055
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.006
dGTP
-
template base: dA, pH 7.5, 37°C
0.0065
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.008
dGTP
pH 7.8, 37°C, incorporation of dGTP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.008
dGTP
-
template base: dI, pH 7.5, 37°C
0.01
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.01
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.01
dGTP
pH 7.8, 37°C, dGTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.014
dGTP
pH 7.8, 37°C, incorporation of dGTP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8ocoG)CACT-5'
0.019
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.019
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.021
dGTP
-
template base: dX, pH 7.5, 37°C
0.027
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.03
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.033
dGTP
-
template base: dG, pH 7.5, 37°C
0.05
dGTP
pH 7.8, 37°C, dGTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.062
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.072
dGTP
-
template base: dT, pH 7.5, 37°C
32
dGTP
-
template base: dC, pH 7.5, 37°C
2470
dGTP
-
pH and temperature not specified in the publication
29500
dGTP
-
pH 8.0, 25°C
28
DNAn
-
in the absence of dNTPs
38
DNAn
-
in the presence of dNTPs
0.0000000019
dTTP
pH 7.5, 37°C, DNA polymerase Dpo2
0.00000012
dTTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.000031
dTTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.00017
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0003
dTTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.0012
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0015
dTTP
-
template base: dC, pH 7.5, 37°C
0.0016
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0026
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0028
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.0031
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0046
dTTP
-
template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C
0.0081
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.01
dTTP
pH 7.8, 37°C, dTTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.016
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.017
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.017
dTTP
-
template base: dI, pH 7.5, 37°C
0.019
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.02
dTTP
pH 7.8, 37°C, dTTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.022
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.024
dTTP
-
template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C
0.037
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.041
dTTP
-
template base: dT, pH 7.5, 37°C
0.044
dTTP
-
template base: dG, pH 7.5, 37°C
0.063
dTTP
-
template base: dX, pH 7.5, 37°C
0.304
dTTP
-
pH 8.0, 22°C, recombinant enzyme
0.83
dTTP
pH 8.8, 30°C, recombinant enzyme, in presence of Mg2+
7.16
dTTP
pH 8.8, 30°C, recombinant enzyme, in presence of Mn2+
9.9
dTTP
-
pH 9.0, 37°C, recombinant mutant enzyme
26
dTTP
-
template base: dA, pH 7.5, 37°C
26.9
dTTP
pH 7.5, 37°C, dTTP insertion opposite the N6dA-(OH)2butyl-GSH in 5'-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3'
30
dTTP
pH 7.5, 37°C, dTTP insertion opposite the N6dA-butanetriol in 5'-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3'
35
dTTP
pH 7.5, 37°C, dTTP insertion opposite an unmodified deoxyadenosine in 5'-TCTCAGTTTATGGACCACC-3'
821.7
dTTP
-
pH 8.0, 22°C, recombinant enzyme
1984
dTTP
-
pH 8.0, 37°C, recombinant enzyme
3692
dTTP
-
pH 8.0, 50°C, recombinant enzyme
18800
dTTP
-
pH 8.0, 25°C
0.04
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.13
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite an abasic site
0.156
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.54
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.625
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
9.2
North-methanocarba-dATP
pH 7.5, 37°C
11.7
North-methanocarba-dATP
pH 7.5, 37°C
additional information
additional information
DNA polymerase IV (Dpo4) shows 90-fold higher incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The catalytic efficiency for these events (with dCTP or C) is similar for G and 8-oxoG templates. Extension beyond an 8-oxoG:C pair is similar to G:C and faster than for an 8-oxoG:A pair, in contrast to other polymerases. dCTP insertion opposite 8-oxoG was lower than for opposite guanine
-
additional information
additional information
-
DNA polymerase IV (Dpo4) shows 90-fold higher incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The catalytic efficiency for these events (with dCTP or C) is similar for G and 8-oxoG templates. Extension beyond an 8-oxoG:C pair is similar to G:C and faster than for an 8-oxoG:A pair, in contrast to other polymerases. dCTP insertion opposite 8-oxoG was lower than for opposite guanine
-
additional information
additional information
-
pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme
-
additional information
additional information
pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme
-
additional information
additional information
-
steady-state kinetic parameters for dCTP incorporation by Dpo4 T239W
-
additional information
additional information
steady-state kinetic parameters for one-base incorporation
-
additional information
additional information
-
steady-state kinetic parameters for one-base incorporation
-
additional information
additional information
steady-state kinetic parameters for one-base incorporation opposite G and N2-alkyl G adducts by Dpo4. Steady-state kinetic parameters for next base extension from G (or N2-alkyl G):C (or T) template primer termini by Dpo4
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additional information
additional information
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steady-state kinetic parameters for one-base incorporation opposite G and N2-alkyl G adducts by Dpo4. Steady-state kinetic parameters for next base extension from G (or N2-alkyl G):C (or T) template primer termini by Dpo4
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additional information
additional information
steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+
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additional information
additional information
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steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+
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additional information
additional information
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steady-state kinetic parameters for the single dNTP incorporation by Dpo4 T239W
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additional information
additional information
transient-state kinetic analysis of Dpo4 mutants and dATP Incorporation opposite 8-oxoG.Transient-state kinetic analysis of Dpo4 mutants and dCTP incorporation opposite 8-oxoG
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additional information
additional information
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transient-state kinetic analysis of Dpo4 mutants and dATP Incorporation opposite 8-oxoG.Transient-state kinetic analysis of Dpo4 mutants and dCTP incorporation opposite 8-oxoG
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additional information
additional information
kinetic for nucleotide insertion opposite template cytosine
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additional information
additional information
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kinetic for nucleotide insertion opposite template cytosine
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additional information
additional information
kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene
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additional information
additional information
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kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene
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additional information
additional information
pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4
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additional information
additional information
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pH 7.5, 50°C, kcat/Km is 0.0225/s * mM for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine
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additional information
additional information
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steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini
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additional information
additional information
steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini
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additional information
additional information
steady-state kinetic parameters for single-nucleotide incorporation opposite the C8- and N2-2-amino-3-methylimidazo[4,5-f]quinoline adducts of dGuo at the G3- and G1-positions of the NarI recognition sequence by Dpo4
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additional information
additional information
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steady-state parameters for the enzyme catalyzed insertion opposite and extension past O2-alkyl-dT
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additional information
additional information
DNA synthesis from dNDPs is a little over an order of magnitude lower than from dNTPs and that Vmax/KM is about 400 times lower for dNDPs than for dNTPs
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