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2.7.7.7: DNA-directed DNA polymerase

This is an abbreviated version!
For detailed information about DNA-directed DNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.7

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
+
DNAn+1

Synonyms

3'–5'-exonuclease, ABO4/POL2a/TIL1, Afu polymerase, ASFV DNA polymerase, ASFV Pol X, B-family replicative DNA polymerase, beta type DNA polymerase, Bst DNA polymerase, CpDNApolI, DBH, Dbh DNA polymerase, Dbh polymerase, ddNTP-sensitive DNA polymerase, Deep Vent DNA polymerase, DeepVent DNA polymerase, deoxynucleate polymerase, deoxyribonucleate nucleotidyltransferase, deoxyribonucleic acid duplicase, deoxyribonucleic acid polymerase, deoxyribonucleic duplicase, deoxyribonucleic polymerase, deoxyribonucleic polymerase I, DinB DNA polymerase, DinB homologue, Dmpol zeta, DNA deoxynucleotidyltransferase, DNA duplicase, DNA nucleotidyltransferase, DNA nucleotidyltransferase (DNA-directed), DNA pol, DNA pol B1, DNA Pol eta, DNA Pol lambda, DNA pol NI, DNA pol Y1, DNA polmerase beta, DNA polymerase, DNA polymerase 1, DNA polymerase 2, DNA polymerase 4, DNA polymerase A, DNA polymerase alpha, DNA polymerase B, DNA polymerase B1, DNA polymerase B2, DNA polymerase B3, DNA polymerase beta, DNA polymerase D, DNA polymerase Dbh, DNA polymerase delta, DNA polymerase Dpo4, DNA polymerase epsilon, DNA polymerase eta, DNA polymerase gamma, DNA polymerase I, DNA polymerase II, DNA polymerase III, DNA polymerase III epsilon subunit, DNA polymerase iota, DNA polymerase IV, DNA polymerase kappa, DNA polymerase lambda, DNA polymerase mu, DNA polymerase ny, DNA polymerase pyrococcus kodakaraensis, DNA polymerase theta, DNA polymerase V, DNA polymerase X, DNA polymerase zeta, DNA polymerases B, DNA polymerases D, DNA polymmerase I, DNA primase-polymerase, DNA replicase, DNA replication polymerase, DNA-dependent DNA polymerase, DNAP, DP1Pho, DP2Pho, Dpo1, Dpo2, Dpo3, Dpo4, Dpo4 polymerase, Dpo4-like enzyme, duplicase, error-prone DNA polymerase, error-prone DNA polymerase X, family B-type DNA polymerase, hoPolD, HSV 1 POL, Igni_0062, K4 polymerase, K4pol, K4PolI, kDNA replication protein, KDO XL DNA polymerase, KF(exo-), KF-, Klenow fragment, Klenow-like DNA polymerase I, KOD DNA polymerases, lesion-bypass DNA polymerase, M1 DNA polymerase, M1pol, MacDinB-1, MA_4027, Miranda pol beta protein, mitochondrial DNA polymerase, Mka polB, More, MsDpo4, mtDNA polymerase NI, mtDNA replicase, Neq DNA polymerase, non-replicative DNA polymerase III, nucleotidyltransferase, deoxyribonucleate, OsPOLP1, PabPol D, PabpolB, PabpolD, Pfu, Pfu DNA polymerase, Pfu Pol, Pfu-POl, PH0121, PH0123, phi29 DNA polymerase, phi29 DNApol, PhoPolD, phPol D, Pol, pol alpha, Pol B, Pol B1, pol beta, Pol BI, pol delta, pol E, POl epsilon, Pol eta, Pol gamma, Pol I, Pol II, pol III, pol iota, Pol IV, pol kappa, pol kappaDELTAC, Pol lambda, Pol mu, pol NI, Pol ny, Pol theta, Pol V, pol Vent (exo-), Pol X, Pol zeta, Pol-beta, POL1, Pol2, POL2a, Pol3, Pol31, PolB, POlB1, polbeta, polD, POLD4, Poldelta, POLdelta1, PolDPho, Polepsilon, Poleta, POLG, PolH, polI, POLIB, POLIC, POLID, poliota, Polkappa, PolX, PolY, poly iota, polymerase alpha catalytic subunit A, polymerase III, pORF30, Pwo DNA polymerase, R2 polymerase, R2 reverse transcriptase, R2-RT, RAD30, RB69 DdDp, RB69 DNA Polymerase, RB69pol, Rec1, repair polymerase, replicative DNA polymerase, reverse transcriptase, RKOD DNA polymerase, Rv1537, Rv3056, Saci_0554, sequenase, Sso, Sso DNA pol B1, Sso DNA pol Y1, Sso DNA polymerase Y1, Sso DNApol, Sso pol B1, SSO0552, SSO2448, SsoDpo1, SsoPolB1, SsoPolY, Szi DNA polymerase, T4 DNA polymerase, T7 DNA polymerase, Taq DNA polymerase, Taq Pol I, Taq polymerase, Tba5 DNA polymerase, Tca DNA polymerase, Tga PolB, TGAM_RS07365, Tkod-Pol, translesion DNA polymerase, translesion DNA synthesis polymerase, translesion polymerase Dpo4, UL30/UL42, UmuD'2C, UmuD'2C-RecA-ATP, Vent polymerase, X family DANN polymerase, Y-family DNA polymerase eta

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.7 DNA-directed DNA polymerase

Molecular Weight

Molecular Weight on EC 2.7.7.7 - DNA-directed DNA polymerase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
100000
100000 - 110000
-
mitochondrial enzyme
101224
x * 101224, calculated from sequence
108000
gel filtration
109000
110000
110000 - 120000
-
HPLC gel filtration, non-denaturing PAGE
110000 - 150000
-
-
114000
Tequatrovirus T4
-
-
115000
12000
-
1 * 12000 thioredoxin + 1 * 84000
120000
120000 - 200000
-
polymerase major
122000
-
DNA polymerase epsilon, gel filtration
124000
-
polymerase delta, calculated from nucleotide sequence
125000
130000
130000 - 280000
-
polymerase alpha
132000 - 200000
-
DNA polymerase epsilon
138000
-
1 * 138000 + 1 * 47000, SDS-PAGE
139700
x * 68100 (polB) + x * 139700 (polc), calculated from sequence
140000
144000
Herpes simplex virus
-
SDS-PAGE
145000
-
gel filtration
150000
150000 - 168000
-
gel filtration, glycerol gradient centrifugation
150000 - 300000
-
polymerase gamma and mitochondrial polymerase
152000
precursor form of DNA polymerase containing 62 kDa intein, SDS-PAGE
152400
about 90000 Da, DNA polymerase precursor form, estimated from amino acid sequence
160000
-
pol III
170000
172000
-
gel filtration
172100
-
gel filtration and sedimentation experiments
178000
-
DNA polymerase delta
180000
182000
-
x * 182000 (alpha) + x * 60000 (beta) + x * 50000 (gamma), core polymerase
185000
-
gel filtration
190000
19960
calculated from amino acid composition, RNase H domain
20000
200000
-
polymerase delta, gel filtration
215000
218000
gel filtration
240000
25000
265000 - 280000
-
dimeric form, polymerase alpha
30000 - 50000
-
polymerase beta
31000
-
DNA polymerase beta
32000
-
x * 140000 (alpha) + x * 37000 (beta) + x * 52000 (gamma) + x * 32000 (delta) + x * 25000 (epsilon) + x * 10000 (theta) + x * 78000 (tau)
35000
-
x * 35000 + x * 125000, ratio 1:1, DNA polymerase gamma
37000
-
x * 140000 (alpha) + x * 37000 (beta) + x * 52000 (gamma) + x * 32000 (delta) + x * 25000 (epsilon) + x * 10000 (theta) + x * 78000 (tau)
38000
-
gel filtration, glycerol gradient centrifugation
39146
x * 39146, purified recombinant detagged enzyme, mass spectrometry
40000
40189
x * 40189, calculated from sequence
42000
-
1 * 215000 + 1 * 42000, polymerase epsilon, denaturing PAGE
421000
gel filtration
43000
-
1 * 43000, SDS-PAGE
44000
Salasvirus phi29
-
C-terminal fragment, SDS-PAGE
45000
-
1 * 45000 + 1 * 60000, SDS-PAGE, mitochondrial enzyme
47000
48000
49000
-
x * 60000 + x * 49000, SDS-PAGE
50000
52000
-
x * 140000 (alpha) + x * 37000 (beta) + x * 52000 (gamma) + x * 32000 (delta) + x * 25000 (epsilon) + x * 10000 (theta) + x * 78000 (tau)
54000
-
1 * 54000 + 1 * 140000
55000
-
x * 125000 + x * 55000, polymerase delta
58000
-
x * 180000 + x * 86000 + x * 58000 + x * 48000, polymerase alpha
60000
62000
-
SDS-PAGE
62865
-
x * 62879, mutant DNA polymerase I, sequence calculation, x * 62865, mutant DNA polymerase I, mass spectrometry
62879
-
x * 62879, mutant DNA polymerase I, sequence calculation, x * 62865, mutant DNA polymerase I, mass spectrometry
63000
-
two-dimensional electrophoresis, second dimension: SDS-PAGE
64000
-
estimated from SDS-PAGE
66000
68100
x * 68100 (polB) + x * 139700 (polc), calculated from sequence
69294
x * 69294 + x + 143161, calculated from sequence
70000
-
polymerase II
75000
Ruellia sp.
-
SDS-PAGE
76000
-
1 * 66000 + 1 * 76000, DNA polymerase alpha, SDS-PAGE
77100
-
N-terminally His6-tagged enzyme fragment Pol ny-77, gel filtration
78000
-
x * 140000 (alpha) + x * 37000 (beta) + x * 52000 (gamma) + x * 32000 (delta) + x * 25000 (epsilon) + x * 10000 (theta) + x * 78000 (tau)
78410
estimated from amino acid sequence
80000 - 130000
-
polymerase I
80000 - 90000
-
glycerol density gradient sedimentation
84000
-
1 * 12000 thioredoxin + 1 * 84000
85000
86000
-
x * 180000 + x * 86000 + x * 58000 + x * 48000, polymerase alpha
87000
-
1 * 155000-175000 + 1 * 87000, SDS-PAGE, polymerase alpha
88000
89000
-
protein band possibly corresponding to mature DNA polymerase, SDS-PAGE
89500
x * 89500, SDS-PAGE
89789
x * 89789, calculated from sequence
89980
mature form of PolB, calculated from amino acid sequence
90054
-
x * 90054, calculated from sequence
91268
x * 91268, calculated from sequence
94000
95000
96000
99000