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2.7.7.9: UTP-glucose-1-phosphate uridylyltransferase

This is an abbreviated version!
For detailed information about UTP-glucose-1-phosphate uridylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.9

Reaction

UTP
+
alpha-D-glucose 1-phosphate
=
diphosphate
 +
UDP-glucose

Synonyms

All3274, CugP, cyanobacterial UDP-Glc PPase, ExoN, GalU, gfugp, Glc-1-P UTase, GlcNAc-1-P UTase, glucose 1-phosphate uridylyltransferase, glucose-1-phosphate urididyltransferase, glucose-1-phosphate uridyltransferase, glucose-1-phosphate uridylyltransferase, KF278717, plastid UDP-glucose pyrophosphorylase, rml-1, ScUGPase-1, sll1558, ST0452, ST0452 protein, STK_04520, sugar-1-P NTase, sugar-1-phosphate nucleotidylyltransferase, TaGalU, UDP glucose pyrophosphorylase, UDP-Glc pyrophosphorylase, UDP-GlcPPase, UDP-glucose pyrophosphorylase, UDP-glucose pyrophosphorylase 1, UDP-glucose pyrophosphorylase isoenzyme UGP5, UDP-glucose pyrophosphorylase UGP1, UDP-glucose pyrophosphorylase UGP2, UDP-glucose pyrophosphorylase UGP3, UDPG phosphorylase, UDPG pyrophosphorylase, UDPG-pyrophosphorylase, UDPglucose pyrophosphorylase, UDPGP, UGP, ugp-1, UGP1, UGP3, UgpA, UGPase, UGPase1, UGPase2, UGPG-PPase, UPD1, uridine 5'-diphosphoglucose pyrophosphorylase, uridine diphosphate-D-glucose pyrophosphorylase, uridine diphosphate-glucose pyrophosphorylase, uridine diphosphoglucose pyrophosphorylase, uridine-diphosphate glucose pyrophosphorylase, uridylyltransferase, glucose 1-phosphate, UTP/dTTP-glucose-1-phosphate uridylyl/thymidylyl transferase, UTP:alpha-D-glucose uridylyltransferase, UTP:glucose-1-phosphate uridylyltransferase, VldB

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.9 UTP-glucose-1-phosphate uridylyltransferase

Cloned

Cloned on EC 2.7.7.9 - UTP-glucose-1-phosphate uridylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, recombinant overepression in Streptococcus zooepidemicus, expression of His-tagged enzyme in Escherichia coli
DNA sequence determination and analysis, gene disruption and depletion of activity is performed, overexpression from multicopy plasmid in haploid strains, on glucose 40fold increased enzyme activity leads to 2fold increase in the concentration of glycogen and UDP-D-glucose, on galactose a 40fold increased activity leads to several effects, e.g. 3fold reduction of growth rate, 3-5fold increase in UDP-D-glucose, UDP-D-galactose and alpha-D-galactose 1-phosphate concentrations, a higher sensitivity to calcofluor white and an increase in the degree of protein glycosylation
-
expressed in Escherichia coli BL21 star
-
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells
expressed in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
expressed in Lactococcus lactis NZ9000
expression in Agrobacterium tumefaciens
expression in chondrocytes with or without co-transfection of HA synthase gene
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
expression of the N-terminally StrepII-tagged enzyme in Escherichia coli BL21(DE3)
-
expression of wild-type and mutants in Escherichia coli JM109
-
from cDNA libraries, 11 clones, DNA and amino acid sequence determination and analysis
from cDNA library, DNA sequence determination and analysis
-
galU structural gene, DNA sequence determination and analysis, expression from multicopy plasmid
-
gene galU, expressio as His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene OsUgp2, DNA and amino acid sequence determination and analysis, promoter sequencing and functional analysis employing deletions and gain-of-function experiments in stable transformants, and expression analysis. The spatio-temporal expression of the gene is mostly codetermined by the types, numbers and positions of cisregulatory elements within the promoter region
gene Ugp, expression in Escherichia coli
heterologous expression in Escherichia coli
-
His-tag version expressed in Escherichia coli BL21 (DE3)
His-tagged version expressed in Escherichia coli BL21 (DE3)
His-tagged version expressed in Escherichia coli BL21(DE3)
His-tagged version expressed in Escherichia coli C41(DE3)
Q94B70
overexpression in Escherichia coli
UGP3 is highly coexpressed with SQD1 and SQD2