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0.09 - 10
alpha-D-galactose 1-phosphate
0.01 - 3.28
alpha-D-glucose 1-phosphate
0.19 - 0.83
D-glucose-1-phosphate
0.32
farnesyl triphosphate
-
pH 8.0, 30°C
0.21
geranyl triphosphate
-
pH 8.0, 30°C
0.51
isopentenyl triphosphate
-
pH 8.0, 30°C
1.1
methylenebisphosphonate
-
pH 8.0, 30°C
4.5
tripolyphosphate
-
30°C, pH 8.0
0.26 - 0.42
UDP-galactose
additional information
additional information
-
0.09
alpha-D-galactose 1-phosphate
cosubstrate UTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
10
alpha-D-galactose 1-phosphate
-
pH 7.5, temperature not specified in the publication
0.01
alpha-D-glucose 1-phosphate
-
pH 7.8, 37°C
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
-
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
pH 7.8
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
pH 7.5, 30°C
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
pH 8.5, 37°C
0.031
alpha-D-glucose 1-phosphate
-
pH 7.5, temperature not specified in the publication
0.03224
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
0.033
alpha-D-glucose 1-phosphate
-
pH 7.5, temperature not specified in the publication
0.0364
alpha-D-glucose 1-phosphate
pH 7.5, 37°C
0.04455
alpha-D-glucose 1-phosphate
-
0.04988
alpha-D-glucose 1-phosphate
cosubstrate: dTTP, pH 7.5, 25°C
0.055 - 0.08
alpha-D-glucose 1-phosphate
-
-
0.055 - 0.08
alpha-D-glucose 1-phosphate
-
pH 7.8
0.055 - 0.08
alpha-D-glucose 1-phosphate
-
at physiological substrate concentration, pH 8.5, 37°C
0.06
alpha-D-glucose 1-phosphate
-
0.067
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant E412K
0.074
alpha-D-glucose 1-phosphate
-
pH 8.35,25°C
0.07765
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
0.085
alpha-D-glucose 1-phosphate
wild-type, pH 7.5, 37°C
0.088
alpha-D-glucose 1-phosphate
mutant A8G, pH 7.5, 37°C
0.095
alpha-D-glucose 1-phosphate
-
-
0.095
alpha-D-glucose 1-phosphate
-
pH 7.8
0.122
alpha-D-glucose 1-phosphate
pH 7.2, temperature not specified in the publication, recombinant enzyme
0.13
alpha-D-glucose 1-phosphate
cosubstrate UTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.14
alpha-D-glucose 1-phosphate
UDP-glucose synthesis
0.14 - 0.18
alpha-D-glucose 1-phosphate
-
-
0.14 - 0.18
alpha-D-glucose 1-phosphate
-
pH 8.0, 30°C
0.151
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant K410S
0.155
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant E412Q
0.183
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.192
alpha-D-glucose 1-phosphate
pH 7.8, 25°C
0.198
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant N491P/L492E
0.202
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant P414G/T415P
0.205
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant E412D
0.217
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant V416N
0.219
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant T406K/M407L
0.226
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.6, 37°C
0.235
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant S309N/S311R
0.24
alpha-D-glucose 1-phosphate
Q94B70
-
0.24
alpha-D-glucose 1-phosphate
pH 7.5, 30°C
0.253
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.316
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.36
alpha-D-glucose 1-phosphate
UDP-glucose synthesis
0.36
alpha-D-glucose 1-phosphate
cosubstrate TTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.44
alpha-D-glucose 1-phosphate
pH 7.0, 50°C
0.46
alpha-D-glucose 1-phosphate
-
-
0.68 - 1
alpha-D-glucose 1-phosphate
-
pH 8.0, 37°C
0.68 - 1
alpha-D-glucose 1-phosphate
-
pH 7.2, 30°C
0.68 - 1
alpha-D-glucose 1-phosphate
-
recombinant wild-type enzyme, pH 8.0, 25°C
0.68 - 1
alpha-D-glucose 1-phosphate
-
at substrate concentrations above 2 mM, pH 8.5, 37°C
0.715
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.742
alpha-D-glucose 1-phosphate
pH 7.5, 37°C
0.83
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.83
alpha-D-glucose 1-phosphate
-
UDP-glucose synthesis
0.9
alpha-D-glucose 1-phosphate
-
pH 9.0, temperature not specified in the publication
1.23
alpha-D-glucose 1-phosphate
wild-type enzyme, pH 7.5, 80°C
1.23
alpha-D-glucose 1-phosphate
-
wild-type enzyme, pH 7.5, 80°C
1.23
alpha-D-glucose 1-phosphate
wild type enzyme, at pH 7.5 and 80°C
1.74
alpha-D-glucose 1-phosphate
mutant enzyme Y97M, pH 7.5, 80°C
1.74
alpha-D-glucose 1-phosphate
-
mutant enzyme Y97M, pH 7.5, 80°C
1.74
alpha-D-glucose 1-phosphate
mutant enzyme Y97M, at pH 7.5 and 80°C
2.5 - 3
alpha-D-glucose 1-phosphate
mutant enzyme Y97N, pH 7.5, 80°C
2.5 - 3
alpha-D-glucose 1-phosphate
-
mutant enzyme Y97N, pH 7.5, 80°C
2.5 - 3
alpha-D-glucose 1-phosphate
mutant enzyme Y97N, at pH 7.5 and 80°C
3.28
alpha-D-glucose 1-phosphate
mutant enzyme Y97V, pH 7.5, 80°C
3.28
alpha-D-glucose 1-phosphate
-
mutant enzyme Y97V, pH 7.5, 80°C
3.28
alpha-D-glucose 1-phosphate
mutant enzyme Y97V, at pH 7.5 and 80°C
0.336
ATP
-
pH 7.6, 70°C
0.276
CTP
-
pH 7.6, 70°C
0.19
D-glucose-1-phosphate
-
-
0.83
D-glucose-1-phosphate
-
-
0.027
diphosphate
Y192A mutant protein
0.03
diphosphate
Ccut-8 mutant protein
0.03
diphosphate
K183A mutant protein
0.03
diphosphate
pyrophosphorolysis
0.034
diphosphate
recombinant wild-type, pH 7.6, 25°C
0.039
diphosphate
wild-type protein
0.04
diphosphate
K332A mutant protein
0.054
diphosphate
-
pH 8.5, 37°C
0.07
diphosphate
pyrophosphorolysis
0.084
diphosphate
-
pH 7.8
0.09
diphosphate
Ncut-21 mutant protein
0.11 - 0.13
diphosphate
-
pH 8.0, 30°C
0.11 - 0.13
diphosphate
-
at physiological substrate concentration, pH 8.5, 37°C
0.17
diphosphate
-
pH 8.35, 25°C
0.18
diphosphate
-
non-galactosemic phenotype, pH 7.8, 37°C
0.2
diphosphate
pH 7.8, 25°C
0.2
diphosphate
-
galactosemic phenotype, pH 7.8, 37°C
0.21
diphosphate
-
pH 7.8
0.24
diphosphate
K405A mutant protein
0.33
diphosphate
-
pH 7.2, 30°C
0.33
diphosphate
-
diphosphate in form of Mg-diphosphate
0.376
diphosphate
pH 7.5, 37°C
0.407
diphosphate
recombinant mutant C99S, pH 7.6, 25°C
0.56
diphosphate
-
pyrophosphorolysis
0.56
diphosphate
-
diphosphate in form of Mg-diphosphate
0.9
diphosphate
Ncut-37 mutant protein
1.66
diphosphate
-
pH 9.0, temperature not specified in the publication
2.4
diphosphate
-
pH 7.6, 37°C
4.9
diphosphate
K260A mutant protein
6.4
diphosphate
Ccut-32 mutant protein
9.8
diphosphate
Ccut-67 mutant protein
29.4
diphosphate
Delta 1-4 mutant protein
33.3
diphosphate
Ccut-101 mutant protein
60
diphosphate
Delta 1-8 mutant protein
0.02422
dTTP
pH 7.5, 25°C
0.366
dTTP
-
pH 7.6, 70°C
0.184
GTP
-
pH 7.6, 37°C
0.58
Mg2+
pyrophosphorolysis direction
0.35
TTP
-
0.9
TTP
S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.26
UDP-galactose
pH 7.5, 30°C
0.42
UDP-galactose
-
pH 7.8
0.00167
UDP-glucose
pH 7.5, temperature not specified in the publication
0.00433
UDP-glucose
pH 7.5, temperature not specified in the publication
0.03
UDP-glucose
Ccut-8 mutant protein
0.03
UDP-glucose
K183A mutant protein
0.03
UDP-glucose
K332A mutant protein
0.031
UDP-glucose
Y192A mutant protein
0.034
UDP-glucose
wild-type protein
0.035
UDP-glucose
-
30°C, pH 8.0
0.04
UDP-glucose
K405A mutant protein
0.04
UDP-glucose
pyrophosphorolysis
0.05 - 0.066
UDP-glucose
-
-
0.05 - 0.066
UDP-glucose
-
-
0.05 - 0.066
UDP-glucose
-
-
0.05 - 0.066
UDP-glucose
-
pH 7.8
0.05 - 0.066
UDP-glucose
-
pH 7.6, 37°C
0.06
UDP-glucose
pyrophosphorolysis
0.065
UDP-glucose
recombinant wild-type, pH 7.6, 25°C
0.074
UDP-glucose
K260A mutant protein
0.101
UDP-glucose
recombinant mutant C99S, pH 7.6, 25°C
0.105
UDP-glucose
pH 7.8, 25°C
0.11
UDP-glucose
pH 7.5, 30°C
0.118
UDP-glucose
Ccut-101 mutant protein
0.12 - 0.18
UDP-glucose
-
pH 8.0, 30°C
0.12 - 0.18
UDP-glucose
-
at physiological substrate concentrations, pH 8.5, 37°C
0.14
UDP-glucose
-
non-galactosemic phenotype, pH 7.8, 37°C
0.18
UDP-glucose
-
galactosemic phenotype, pH 7.8, 37°C
0.19
UDP-glucose
-
pH 8.35, 25°C
0.31
UDP-glucose
Ccut-32 mutant protein
0.362
UDP-glucose
Ccut-67 mutant protein
0.503
UDP-glucose
Delta 1-8 mutant protein
0.555
UDP-glucose
Delta 1-4 mutant protein
0.68
UDP-glucose
-
pyrophosphorolysis
0.774
UDP-glucose
-
pH 7.5, temperature not specified in the publication
1.1
UDP-glucose
Ncut-21 mutant protein
3.04
UDP-glucose
pH 7.5, 37°C
57
UDP-glucose
Ncut-37 mutant protein
0.0075
UTP
-
isoenzyme UgpG, 30°C, pH 7.8
0.025
UTP
-
pH 7.5, temperature not specified in the publication
0.053
UTP
pH 7.2, temperature not specified in the publication, recombinant enzyme
0.058 - 0.07
UTP
-
pH 8.0, 37°C
0.058 - 0.07
UTP
-
pH 7.8, 37°C
0.08
UTP
UDP-glucose synthesis
0.08
UTP
cosubstrate alpha-D-galactose 1-phosphate, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.093
UTP
-
pH 8.35, 25°C
0.1 - 0.2
UTP
-
pH 7.5, 30°C
0.1 - 0.2
UTP
-
pH 8.0, 30°C
0.1 - 0.2
UTP
-
at physiological substrate concentrations, pH 8.5, 37°C
0.14
UTP
S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.17
UTP
UDP-glucose synthesis
0.18
UTP
wild-type, pH 7.5, 37°C
0.22
UTP
-
UDP-glucose synthesis
0.22
UTP
-
UTP in form of MgUTP
0.229
UTP
-
isoenzyme RmlA, 30°C, pH 7.8
0.35
UTP
mutant A8G, pH 7.5, 37°C
0.53
UTP
-
at substrate concentrations above 2 mM, pH 8.5, 37°C
0.56
UTP
-
recombinant wild-type enzyme, pH 8.0, 25°C
0.56
UTP
-
UTP in form of MgUTP
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
two-substrate kinetics
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-