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2.7.7.B22: transposase

This is an abbreviated version!
For detailed information about transposase, go to the full flat file.

Word Map on EC 2.7.7.B22

Reaction

binds to the ends of a transposon (DNA that moves) and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism =

Synonyms

Ac transposase, AcTPase, APM_2825, Buster, DbuzGalileo, DDE-type integrase/transposase/recombinase, DDE_Tnp_1_7 domain-containing protein, DNA transposase, ECO103_3558, Galileo transposase, hAT transposase, hAT-transposase 1, Hermes, Hermes transposase, Hsmar1 transposase, ICE6013, IS10 transposase, IS1341-type transposase, IS1634 transposase, IS200 transposase, IS30-like transposase, IS608, IS711, ISC1041, Kat1, maize activator transposase, mariner transposase, Mbar_A1742, PGM, piggyBac transposase, PiggyMac, Rev 1 transposase, Rev 2 transposase, SB transposase, SB100X, SETMAR, sleeping beauty transposase, SSO1474, Ta0474, Tgf2 TPase, Tgf2 transposase, Tgf2TPase, Tn4430 TnpA, Tn5, Tn5 transposase, Tn7 transposase, TnpA, TnpA 155-amino acid transposase, TnpA transposase, TnsA, TnsB, Tol2, Tpase, transposase for transposon Tn4430, transposase for transposon Tn5, wbk IS transposase, wbkA flanking transposase, wbkA-flanking IS

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.B22 transposase

Reference

topprint

Reference on EC 2.7.7.B22 - transposase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kang, J.Y.; Lee, H.H.; Kim, do J.; Han, S.H.; Kim, O.; Kim, H.S.; Lee, S.J.; Suh, S.W.
Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene
Acta Crystallogr. Sect. F
62
1147-1149
2006
Thermoplasma acidophilum (Q9HKW9), Thermoplasma acidophilum
Manually annotated by BRENDA team
Ammendola, S.; Politi, L.; Scandurra, R.
Cloning and sequencing of ISC1041 from the archaeon Sulfolobus solfataricus MT-4, a new member of the IS30 family of insertion elements
FEBS Lett.
428
217-223
1998
Saccharolobus solfataricus (O74034), Saccharolobus solfataricus MT-4 / DSM 5833 (O74034)
Manually annotated by BRENDA team
Lee, H.H.; Yoon, J.Y.; Kim, H.S.; Kang, J.Y.; Kim K.H.; Kim, D.J.; Ha, J.Y.; Mikami, B.; Yoon, H.J.; Suh, S.W.
Crystal structure of a metal ion-bound IS200 transposase
J. Biol. Chem.
281
4261-4266
2005
Saccharolobus solfataricus (Q97Y68), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97Y68)
Manually annotated by BRENDA team
Thakker, C.; Lin, K.; Martini-Stoica, H.; Bennett, G.N.
Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli
Biotechnol. J.
11
80-90
2016
Escherichia coli O103:H2, Escherichia coli O103:H2 EHEC
Manually annotated by BRENDA team
Hickman, A.B.; Ewis, H.E.; Li, X.; Knapp, J.A.; Laver, T.; Doss, A.L.; Tolun, G.; Steven, A.C.; Grishaev, A.; Bax, A.; Atkinson, P.W.; Craig, N.L.; Dyda, F.
Structural basis of hAT transposon end recognition by Hermes, an octameric DNA transposase from Musca domestica
Cell
158
353-367
2014
Musca domestica (Q25442), Musca domestica
Manually annotated by BRENDA team
Marzo, M.; Liu, D.; Ruiz, A.; Chalmers, R.
Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats
Gene
525
84-91
2013
Drosophila buzzatii
Manually annotated by BRENDA team
Shen, X.D.; Hou, F.; Chen, J.; Jiang, X.Y.; Zou, S.M.
Identification of nuclear localization signal within goldfish Tgf2 transposase
Gene
593
21-27
2016
Carassius auratus (H9AER4), Carassius auratus
Manually annotated by BRENDA team
Lamb-Palmer, N.; Singh, M.; Dalton, J.; Singh, J.
Prokaryotic expression and purification of soluble maize Ac transposase
Mol. Biotechnol.
54
685-691
2013
Zea mays (P08770), Zea mays
Manually annotated by BRENDA team
Xu, H.L.; Shen, X.D.; Hou, F.; Cheng, L.D.; Zou, S.M.; Jiang, X.Y.
Prokaryotic expression and purification of soluble goldfish Tgf2 transposase with transposition activity
Mol. Biotechnol.
57
94-100
2015
Carassius auratus (H9AER4), Carassius auratus
Manually annotated by BRENDA team
Ellis, M.J.; Trussler, R.S.; Haniford, D.B.
Hfq binds directly to the ribosome-binding site of IS10 transposase mRNA to inhibit translation
Mol. Microbiol.
96
633-650
2015
Escherichia coli
Manually annotated by BRENDA team
Escobar, H.; Schoewel, V.; Spuler, S.; Marg, A.; Izsvak, Z.
Full-length dysferlin transfer by the hyperactive sleeping beauty transposase restores dysferlin-deficient muscle
Mol. Ther. Nucleic Acids
5
e277
2016
Homo sapiens
Manually annotated by BRENDA team
Abrusan, G.; Yant, S.R.; Szilagyi, A.; Marsh, J.A.; Mates, L.; Izsvak, Z.; Barabas, O.; Ivics, Z.
Structural determinants of sleeping beauty transposase activity
Mol. Ther.
24
1369-1377
2016
Homo sapiens
Manually annotated by BRENDA team
Voigt, F.; Wiedemann, L.; Zuliani, C.; Querques, I.; Sebe, A.; Mates, L.; Izsvak, Z.; Ivics, Z.; Barabas, O.
Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
Nat. Commun.
7
11126
2016
Homo sapiens
Manually annotated by BRENDA team
Leighton, G.O.; Konnova, T.A.; Idiyatullin, B.; Hurr, S.H.; Zuev, Y.F.; Nesmelova, I.V.
The folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA
PLoS ONE
9
e112114
2014
Homo sapiens
Manually annotated by BRENDA team
Choi, K.Y.; Spencer, J.M.; Craig, N.L.
The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD
Proc. Natl. Acad. Sci. USA
111
E2858-E2865
2014
Escherichia coli (P13988 AND P13989)
Manually annotated by BRENDA team
Carpentier, C.E.; Schreifels, J.M.; Aronovich, E.L.; Carlson, D.F.; Hackett, P.B.; Nesmelova, I.V.
NMR structural analysis of Sleeping Beauty transposase binding to DNA
Protein Sci.
23
23-33
2014
Homo sapiens
Manually annotated by BRENDA team
Gomes-Filho, J.V.; Zaramela, L.S.; Italiani, V.C.; Baliga, N.S.; Vencio, R.Z.; Koide, T.
Sense overlapping transcripts in IS1341-type transposase genes are functional non-coding RNAs in archaea
RNA Biol.
12
490-500
2015
Halobacterium salinarum, Halobacterium salinarum NRC 1
Manually annotated by BRENDA team
Diaz-Maldonado, H.; Gomez, M.J.; Moreno-Paz, M.; San Martin-Uriz, P.; Amils, R.; Parro, V.; Lopez de Saro, F.J.
Transposase interaction with the beta sliding clamp: effects on insertion sequence proliferation and transposition rate
Sci. Rep.
5
13329
2015
Acidiphilium sp. PM (F7S919), Methanosarcina barkeri (Q465C2), Methanosarcina barkeri DSM 804 (Q465C2)
Manually annotated by BRENDA team
Jiang, X.Y.; Hou, F.; Shen, X.D.; Du, X.D.; Xu, H.L.; Zou, S.M.
The N-terminal zinc finger domain of Tgf2 transposase contributes to DNA binding and to transposition activity
Sci. Rep.
6
27101
2016
Carassius auratus (H9AER4), Carassius auratus
Manually annotated by BRENDA team
Mancilla, M.; Grillo, M.J.; de Miguel, M.J.; Lopez-Goni, I.; San-Roman, B.; Zabalza-Barangua, A.; Moriyon, I.
Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine
Vet. Res.
44
105
2013
Brucella melitensis, Brucella melitensis Rev1
Manually annotated by BRENDA team
Inaba, Y.; Banerjee, I.; Kernan, T.; Banta, S.
Transposase-mediated chromosomal integration of exogenous genes in Acidithiobacillus ferrooxidans
Appl. Environ. Microbiol.
84
e01381
2018
Escherichia coli (Q46731)
Manually annotated by BRENDA team
Sharma, R.; Nirwal, S.; Narayanan, N.; Nair, D.T.
Dimerization through the RING-finger domain attenuates excision activity of the piggyBac transposase
Biochemistry
57
2913-2922
2018
Trichoplusia ni (Q27026)
Manually annotated by BRENDA team
Kia, A.; Gloeckner, C.; Osothprarop, T.; Gormley, N.; Bomati, E.; Stephenson, M.; Goryshin, I.; He, M.M.
Improved genome sequencing using an engineered transposase
BMC Biotechnol.
17
006
2017
Escherichia coli (Q46731)
Manually annotated by BRENDA team
Lu, B.; Dong, L.; Yi, D.; Zhang, M.; Zhu, C.; Li, X.; Yi, C.
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife
9
1-16
2020
Escherichia coli (Q46731)
Manually annotated by BRENDA team
Sansevere, E.; Luo, X.; Park, J.; Yoon, S.; Seo, K.; Robinson, D.
Transposase-mediated excision, conjugative transfer, and diversity of ICE6013 elements in Staphylococcus aureus
J. Bacteriol.
199
e00629
2017
Staphylococcus aureus (B6V378)
Manually annotated by BRENDA team
Ni, J.; Wangensteen, K.; Nelsen, D.; Balciunas, D.; Skuster, K.; Urban, M.; Ekker, S.
Active recombinant Tol2 transposase for gene transfer and gene discovery applications
Mob. DNA
7
006
2016
Oryzias latipes (Q9PTV0 and Q9PTV1), Oryzias latipes
Manually annotated by BRENDA team
Deniger, D.C.; Pasetto, A.; Tran, E.; Parkhurst, M.R.; Cohen, C.J.; Robbins, P.F.; Cooper, L.J.; Rosenberg, S.A.
Stable, nonviral expression of mutated tumor neoantigen-specific T-cell receptors using the sleeping beauty transposon/transposase system
Mol. Ther.
24
1078-1089
2016
Homo sapiens
Manually annotated by BRENDA team
Boehme, P.; Zhang, W.; Solanki, M.; Ehrke-Schulz, E.; Ehrhardt, A.
A high-capacity adenoviral hybrid vector system utilizing the hyperactive sleeping beauty Transposase SB100X for enhanced integration
Mol. Ther. Nucleic Acids
5
e337
2016
Homo sapiens
Manually annotated by BRENDA team
Querques, I.; Mades, A.; Zuliani, C.; Miskey, C.; Alb, M.; Grueso, E.; Machwirth, M.; Rausch, T.; Einsele, H.; Ivics, Z.; Hudecek, M.; Barabas, O.
A highly soluble Sleeping Beauty transposase improves control of gene insertion
Nat. Biotechnol.
37
1502-1512
2019
Homo sapiens
Manually annotated by BRENDA team
Chen, Q.; Luo, W.; Veach, R.; Hickman, A.; Wilson, M.; Dyda, F.
Structural basis of seamless excision and specific targeting by piggyBac transposase
Nat. Commun.
11
3446
2020
Trichoplusia ni (Q27026)
Manually annotated by BRENDA team
Claeys Bouuaert, C.; Chalmers, R.
A single active site in the mariner transposase cleaves DNA strands of opposite polarity
Nucleic Acids Res.
45
11467-11478
2017
Homo sapiens (Q53H47)
Manually annotated by BRENDA team
Dubois, E.; Mathy, N.; Regnier, V.; Bischerour, J.; Baudry, C.; Trouslard, R.; Betermier, M.
Multimerization properties of PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements
Nucleic Acids Res.
45
3204-3216
2017
Paramecium tetraurelia
Manually annotated by BRENDA team
Woodard, L.E.; Downes, L.M.; Lee, Y.C.; Kaja, A.; Terefe, E.S.; Wilson, M.H.
Temporal self-regulation of transposition through host-independent transposase rodlet formation
Nucleic Acids Res.
45
353-366
2017
Tribolium castaneum
Manually annotated by BRENDA team
Hickman, A.B.; Voth, A.R.; Ewis, H.; Li, X.; Craig, N.L.; Dyda, F.
Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase
Nucleic Acids Res.
46
10286-10301
2018
Musca domestica (Q25438)
Manually annotated by BRENDA team
Morellet, N.; Li, X.; Wieninger, S.A.; Taylor, J.L.; Bischerour, J.; Moriau, S.; Lescop, E.; Bardiaux, B.; Mathy, N.; Assrir, N.; Betermier, M.; Nilges, M.; Hickman, A.B.; Dyda, F.; Craig, N.L.; Guittet, E.
Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase
Nucleic Acids Res.
46
2660-2677
2018
Trichoplusia ni (Q27026)
Manually annotated by BRENDA team
Sato, S.; Arimura, Y.; Kujirai, T.; Harada, A.; Maehara, K.; Nogami, J.; Ohkawa, Y.; Kurumizaka, H.
Biochemical analysis of nucleosome targeting by Tn5 transposase
Open biology
9
190116
2019
Escherichia coli (Q46731)
Manually annotated by BRENDA team
Nicolas, E.; Oger, C.A.; Nguyen, N.; Lambin, M.; Draime, A.; Leterme, S.C.; Chandler, M.; Hallet, B.F.
Unlocking Tn3-family transposase activity in vitro unveils an asymetric pathway for transposome assembly
Proc. Natl. Acad. Sci. USA
114
E669-E678
2017
Bacillus thuringiensis (P10021)
Manually annotated by BRENDA team
Chiruvella, K.K.; Rajaei, N.; Jonna, V.R.; Hofer, A.; Astroem, S.U.
Biochemical characterization of Kat1 a domesticated hAT-transposase that induces DNA hairpin formation and MAT-switching
Sci. Rep.
6
21671
2016
Kluyveromyces lactis
Manually annotated by BRENDA team