2.7.7.B5: adenylyltransferase ThiI
This is an abbreviated version!
For detailed information about adenylyltransferase ThiI, go to the full flat file.
Reaction
Synonyms
ThiI
ECTree
2.7.7.B5 adenylyltransferase ThiIAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.7.B5 - adenylyltransferase ThiI
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + TPHE39A
adenylated TPHE39A + diphosphate
-
truncated tRNA consisting of 39 nucleotides derived from tRNAPhe, minimal RNA substrate for modification by ThiI. The crystal structure of TPHE39A shows that base pairs in the T-stem are almost completely disrupted, although those in the acceptor-stem are preserved. ThiI can efficiently bind with not only tRNAPhe but also TPHE39A
-
-
?
tRNA-uridine + GTP
adenylated-trNA-uridine + diphosphate
-
kcat/Km for GTP is about 6fold lower than the value for ATP
-
-
?
tRNA-uridine + ATP
adenylated-trNA-uridine + diphosphate
-
based upon sequence alignments, ThiI shares a unique P-loop motif with the PPi synthetase family. ThiI very likely catalyzes the adenylation of a substrate, probably a molecule bearing a carbonyl oxygen
-
-
?
additional information
?
-
-
ThiI is involved in the modification of uridine to 4-thiouridine
-
-
?
additional information
?
-
-
ThiI plays a role in the transfer of sulfur from cysteine to both thiamine and 4-thiouridine
-
-
?
