Information on EC 1.1.1.120 - galactose 1-dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.120
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RECOMMENDED NAME
GeneOntology No.
galactose 1-dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-galactose + NADP+ = D-galactono-1,5-lactone + NADPH + H+
show the reaction diagram
Also acts on L-arabinose, 6-deoxy- and 2-deoxy-D-galactose
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
D-galactose:NADP+ 1-oxidoreductase
Also acts on L-arabinose, 6-deoxy- and 2-deoxy-D-galactose.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-51-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
mutant strain G6
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,6-dideoxy-D-allofuranose + NADP+
? + NADPH
show the reaction diagram
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i.e. digitoxose, furanose form, 81% of the activity compared to abequose
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?
2-deoxy-D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
2-deoxy-D-ribose + NADP+
? + NADPH
show the reaction diagram
-
18% of the activity compared to abequose
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?
3,6-dideoxy-beta-D-galactofuranose + NADP+
3,6-dideoxy-D-galactono-1,4-lactone + NADPH
show the reaction diagram
-
i.e. abequose, furanose form, beta-configuration
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?
3-deoxy-D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
-
36% of the activity compared to abequose
-
?
6-deoxy-D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
alpha,beta-L-arabinose + NADP+
L-arabinolactone + NADPH
show the reaction diagram
alpha-D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
-
39% of the activity compared to alpha,beta-D-galactose and alpha,beta-L-arabinose
product is assumed to be the corresponding -1,5-lactone
?
beta-D-galactofuranose + NADP+
D-galactono-1,4-lactone + NADPH
show the reaction diagram
beta-D-galactose + NADP+
D-galatonolactone + NADPH
show the reaction diagram
-
-
product is assumed to be the corresponding -1,5-lactone
?
beta-L-arabinose + NADP+
L-arabinolactone + NADPH
show the reaction diagram
D-arabinose + NADP+
D-arabinolactone + NADPH
show the reaction diagram
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5% of the activity compared to abequose
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?
D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
D-ribose + NADP+
? + NADPH
show the reaction diagram
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traces of activity
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?
L-arabinose + NADP+
L-arabinolactone + NADPH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
-
39% of the activity compared to alpha,beta-D-galactose and alpha,beta-L-arabinose
product is assumed to be the corresponding -1,5-lactone
?
beta-D-galactose + NADP+
D-galatonolactone + NADPH
show the reaction diagram
-
-
product is assumed to be the corresponding -1,5-lactone
?
D-galactose + NADP+
D-galactonolactone + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
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82% inhibition at 20 mM
glutathione
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27% inhibition at 20 mM
Hg2+
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complete inhibition at 1 mM
p-chloromercuribenzoate
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complete inhibition at 1 mM, partial reversible by low concentration of cysteine
thioglycolate
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96% inhibition at 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
130
2,6-dideoxy-D-allose
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12
2-deoxy-D-galactose
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3.3
3,6-Dideoxy-D-galactose
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48
3-Deoxy-D-galactose
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60
6-deoxy-D-galactose
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420
D-galactose
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0.037
NADP+
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.88
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substrate: D-galactose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.5
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substrate: abequose
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, 40% saturation
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very stable at -15°C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, ammonium sulfate precipitate, many months, without appreciable loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
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