Information on EC 1.1.1.151 - 21-hydroxysteroid dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.151
-
RECOMMENDED NAME
GeneOntology No.
21-hydroxysteroid dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pregnan-21-ol + NADP+ = pregnan-21-al + NADPH + H+
show the reaction diagram
Acts on a number of 21-hydroxycorticosteroids
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
21-hydroxysteroid:NADP+ 21-oxidoreductase
Acts on a number of 21-hydroxycorticosteroids.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-76-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ovis aries aries
sheep
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADPH
11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NADP+
show the reaction diagram
Ovis aries aries
-
i.e. 21-dehydro-delta1-cortisol
21-hydroxy-delta1-cortisol
?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADPH
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+
show the reaction diagram
Ovis aries aries
-
i.e. 21-dehydrocortisol
i.e. cortisol
?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADPH
11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+
show the reaction diagram
Ovis aries aries
-
i.e. 21-dehydrocorticosterone
21-hydroxycorticosterone
?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADPH
17,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+
show the reaction diagram
Ovis aries aries
-
i.e. 21-dehydro-11-deoxycortisol
i.e. 21-hydroxy-11-deoxycortisol
?
17alpha-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH
17alpha,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+
show the reaction diagram
Ovis aries aries
-
i.e. 21-dehydrocortisone, marked dependence on the nature of steroids
i.e. cortisone
?
pregn-4-ene-3,20,21-trione + NADPH
21-hydroxy-pregn-4-ene-3,20-dione + NADP+
show the reaction diagram
Ovis aries aries
-
i.e. 21-dehydro-11-deoxy-corticosterone
21-hydroxy-11-deoxy-corticosterone
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
Ovis aries aries
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
Ovis aries aries
-
complete inhibition at 0.004 mM
Hg2+
Ovis aries aries
-
53% inhibition at 0.002 mM
NADH
Ovis aries aries
-
competitive inhibition
NADPH
Ovis aries aries
-
competitive inhibition
p-mercuribenzoate
Ovis aries aries
-
55% inhibition at 0.014 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.137
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
Ovis aries aries
-
-
0.177
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
Ovis aries aries
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-
0.233
11beta-hydroxy-pregn-4-ene-3,20,21-trione
Ovis aries aries
-
-
0.461
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
Ovis aries aries
-
-
0.137
17-hydroxy-pregn-4-ene-3,20,21-trione
Ovis aries aries
-
-
0.021
NADPH
Ovis aries aries
-
-
0.118
pregn-4-ene-3,20,21-trione
Ovis aries aries
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 6.1
Ovis aries aries
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-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Ovis aries aries
-
-
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
Ovis aries aries
-
80% loss of activity in 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
not sensitive against repeated freezing and thawing
Ovis aries aries
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 0.01 M sodium phosphate , pH 6-7.4, 1 year
Ovis aries aries
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, Sephadex G-100, starch block elelctrophoresis
Ovis aries aries
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