Information on EC 1.1.1.162 - erythrulose reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.162
-
RECOMMENDED NAME
GeneOntology No.
erythrulose reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-threitol + NADP+ = D-erythrulose + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-threitol:NADP+ oxidoreductase
NAD+ is also utilized, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
52064-49-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1S)-(+)-camphorquinone + NADPH
?
show the reaction diagram
-
stereospecific
-
-
?
1,2-cyclohexanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
2,3-heptanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
2,3-pentanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
3,4-hexanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
acetoin + NADPH + H+
2,3-butanediol + NADP+
show the reaction diagram
-
only slight reversibility, low activity
-
r
D-erythrulose + NAD(P)+
?
show the reaction diagram
-
one route of formation of tetritols in mammalia
-
-
r
D-erythrulose + NAD(P)H
D-threitol + NAD(P)+
show the reaction diagram
diacetyl + NAD(P)H
acetoin + NADP+
show the reaction diagram
ethyl pyruvate + NADPH
ethyllactate + NADP+
show the reaction diagram
-
-
-
-
?
isatin + NADPH
?
show the reaction diagram
-
s-cis configuration of the neighbouring carbonyls is important for activity
-
-
?
methyl pyruvate + NADPH
methyllactate + NADP+
show the reaction diagram
-
-
-
-
?
Phenanthrenequinone + NADPH
?
show the reaction diagram
-
s-cis configuration of the neighbouring carbonyls is important for activity
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-erythrulose + NAD(P)+
?
show the reaction diagram
-
one route of formation of tetritols in mammalia
-
-
r
diacetyl + NAD(P)H
acetoin + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',5'-ADP
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.68
(1S)-(+)-Camphorquinone
-
-
0.67
1,2-Cyclohexanedione
-
-
0.26
2,3-heptanedione
-
-
0.24
2,3-Pentanedione
-
-
0.19
3,4-hexanedione
-
-
13
acetoin
-
-
0.11 - 0.38
D-erythrulose
0.39
diacetyl
-
-
1.1
ethyl pyruvate
-
-
0.72
isatin
-
-
1.1
Methyl pyruvate
-
-
0.067 - 0.22
NADH
0.0068 - 0.0079
NADPH
0.021
Phenanthrenequinone
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.021
-
acetoin
64
-
(1S)-(+)-camphorquinone
71.3
-
-
77
-
methyl pyruvate
83
-
phenanthrenequione
120
-
1,2-cyclohexanedione; 2,3-pentanedione; ethyl pyruvate
123
-
-
130
-
diacetyl
180
-
2,3-heptanedione
200
-
3,4-hexanedione
250
-
isatin
320
-
purified enzyme, D-erythrulose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 6
-
diacetyl + NADPH
5.8
-
D-erythrulose + NADPH
5.85
-
D-erythrulose + NADH
6 - 6.8
-
diacetyl + NADH
6.25
-
D-erythrulose + NADH
6.3
-
D-erythrulose + NADH
9
-
D-threitol + NADP+
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.25 - 8
7.5 - 9
-
oxidation of acetoin to 2,3-butanediol
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
-
sedimentation equilibrium analysis, gel filtration, sucrose density gradient centrifugation
96000
-
sedimentation equilibrium analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
-
comparative modeling of enzyme based on structure of tetrameric carbonyl reductase
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
probably acetylated methionine at N-terminal
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
maximal stability
286089
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
23.5 h, 90% loss of activity, regains about 55-65% of its original activity after 60 min
12
-
inactivated below
21
-
23.5 h, 20-25% loss of activity
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2,5'-ADP protects against Rose bengal-sensitized photoinactivation, to a lesser extent than NADP+
-
NADP+ protects against heat inactivation or Rose bengal-sensitized photoinactivation and cold inactivation
-
NADP+ protects against pH inactivation
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation, in presence of Rose bengal, protection by NADP+ and to a lesser extent by 2',5'-ADP
-
286086, 286090
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, as crystalline suspension in 1.43 mM ammonium sulfate, pH 8.2, 15 mM dithiothreitol, 0.2 mM NADP+, several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis