Information on EC 1.1.1.165 - 2-alkyn-1-ol dehydrogenase

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The expected taxonomic range for this enzyme is: Fusarium merismoides

EC NUMBER
COMMENTARY hide
1.1.1.165
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RECOMMENDED NAME
GeneOntology No.
2-alkyn-1-ol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-butyne-1,4-diol + NAD+ = 4-hydroxy-2-butynal + NADH + H+
show the reaction diagram
Acts on a variety of 2-alkyn-1-ols, and also on 1,4-butanediol. NADP+ also acts as acceptor, but more slowly
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
2-butyne-1,4-diol:NAD+ 1-oxidoreductase
Acts on a variety of 2-alkyn-1-ols, and also on 1,4-butanediol. NADP+ also acts as acceptor, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
54576-94-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strainB11
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Manually annotated by BRENDA team
strainB11
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2,4-butanetriol + NAD+
? + NADH
show the reaction diagram
1,2-butanediol + NAD+
1-hydroxybutan-2-one + NADH
show the reaction diagram
1,2-propanediol + NAD+
1-hydroxy-2-propanal + NADH
show the reaction diagram
1,4-butanediol + NAD+
1-hydroxy-4-butanal + NADH
show the reaction diagram
1,6-hexanediol + NAD+
1-hydroxy-6-hexanal + NADH
show the reaction diagram
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low activity
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?
1-butene-3-ol + NAD+
1-buten-3-one + NADH
show the reaction diagram
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low activity
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?
1-butyne-3-ol + NAD+
1-butyne-3-one + NADH
show the reaction diagram
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very low activity
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?
2-butene-1-ol + NAD+
2-butene-1-al + NADH
show the reaction diagram
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cis-trans mixture, very low activity
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?
2-butyne-1,4-diol + NAD+
4-hydroxybut-2-ynal + NADH
show the reaction diagram
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?
2-butyne-1-ol + NAD+
2-butyn-1-al + NADH
show the reaction diagram
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highest activity
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?
2-propene-1-ol + NAD+
2-propene-1-al + NADH
show the reaction diagram
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low activity
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?
2-propyne-1-ol + NAD+
2-propyn-1-al + NADH
show the reaction diagram
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?
3-butyne-1-ol + NAD+
3-butyn-1-al + NADH
show the reaction diagram
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very low activity
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?
n-propanol + NAD+
n-propanal + NADH
show the reaction diagram
trans-2-butene-1,4-diol + NAD+
? + NADH
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-butyne-1,4-diol + NAD+
4-hydroxybut-2-ynal + NADH
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
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one third of the activity compared to NAD+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CuCl2+
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complete inhibition at 2 mM
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HgCl2
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complete inhibition at 2 mM
iodoacetic acid
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complete inhibition at 2 mM
KCN
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slight inhibition at 2 mM
NaN3
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slight inhibition at 2 mM
Ni2+
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84% inhibition at 2 mM
p-chloromercuribenzoate
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complete inhibition at 2 mM
Zn2+
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55% inhibition at 2 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.1
2-butyne-1,4-diol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.5
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stable between
286099
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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stable below, rapid decrease of activity above
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
highly labile, even in the presence of DTT
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
5°C, 2 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
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