Information on EC 1.1.1.168 - 2-dehydropantolactone reductase (Re-specific)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.168
-
RECOMMENDED NAME
GeneOntology No.
2-dehydropantolactone reductase (Re-specific)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-pantolactone + NADP+ = 2-dehydropantolactone + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
phosphopantothenate biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-pantolactone:NADP+ oxidoreductase (Re-specific)
The yeast enzyme differs from that from Escherichia coli [EC 1.1.1.214 2-dehydropantolactone reductase (Si-specific)], which is specific for the Si-face of NADP+, and in receptor requirements from EC 1.1.99.26 3-hydroxycyclohexanone dehydrogenase.
CAS REGISTRY NUMBER
COMMENTARY hide
37211-75-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
IFO 0708
-
-
Manually annotated by BRENDA team
AKU 3006
-
-
Manually annotated by BRENDA team
AKU 3006
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methylisatin + NADPH
?
show the reaction diagram
2-dehydropantoyl lactone + NADPH
(R)-pantoyl lactone + NADP+
show the reaction diagram
5-bromoisatin + NADPH
?
show the reaction diagram
-
-
-
-
?
5-methylisatin + NADPH
?
show the reaction diagram
8-bromobornanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
alloxan + NADPH
?
show the reaction diagram
-
-
-
-
?
beta-naphthoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
bornanedione + NADPH
?
show the reaction diagram
camphorquinone + NADPH
?
show the reaction diagram
dihydro-4,4-diethyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
dihydro-4-methyl-4-propyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione + NADPH
?
show the reaction diagram
-
-
-
-
?
isatin + NADPH
?
show the reaction diagram
ketopantoyl lactone + NADPH
?
show the reaction diagram
ketopantoyl lactone + NADPH
D-(-)-pantoyl lactone
show the reaction diagram
-
and its derivatives
-
?
naphthoquinone + NADPH
?
show the reaction diagram
ninhydrin + NADPH
?
show the reaction diagram
-
-
-
-
?
oxopantoyl lactone + NADPH
?
show the reaction diagram
-
-
-
-
?
additional information
additional information
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-dehydropantoyl lactone + NADPH
(R)-pantoyl lactone + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
strictly dependent on
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-Cyclopentanedione
-
-
2-keto-4-hydroxybutyrolactone
-
-
2-Oxo-4-hydroxy-3-methylbutyric acid-gamma-lactone
-
-
2-Oxo-4-hydroxybutyric acid-gamma-lactone
-
-
2-oxoisopentanoic acid
-
-
2-Oxopentanoic acid
-
-
3,4-Dihydroxy-3-cyclobutene-1,2-dione
-
uncompetitive to isatin
iodoacetate
-
-
ketopantoyl lactone
oxopyrrolidines
-
-
p-chloromercuribenzoate
-
-
Polyketones
-
quercetin
Reactive Red 3A
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0057 - 0.085
1-methylisatin
0.0031
5-bromoisatin
-
-
0.0083 - 0.016
5-methylisatin
0.015
8-bromobornanedione
-
-
0.83
alloxan
-
-
0.093
beta-naphthoquinone
-
-
0.012
bornanedione
-
-
0.42
dihydro-4,4-diethyl-2,3-furanedione
-
-
0.983
dihydro-4-methyl-4-propyl-2,3-furanedione
-
-
0.043
dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione
-
-
0.013
dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione
-
-
0.09
dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione
-
-
0.0098
dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione
-
-
0.0093
dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione
-
-
0.064
dl-camphorquinone
-
-
0.0066 - 0.014
isatin
0.031 - 0.333
ketopantoyl lactone
0.039 - 0.062
NADPH
0.52
ninhydrin
-
-
0.026
oxopantoyl lactone
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0025
Reactive Red 3A
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
173
-
ketopantoyl lactone
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1 - 5.6
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 8
-
at pH 5.2 and 8.0 about 50% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000 - 36000
-
gel filtration, SDS-PAGE
39000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
30°C, 30 min, 33% loss of activity
347724
6 - 10
-
-
347724
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
pH 7.0, 10 min, 58% loss of activity
56
-
0.35 M anhydrous sodium sulfate, complete inactivation
60
-
pH 7.0, 10 min, 77% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 year, 15% loss of activity
-
4°C, 1 year, 50% glycerol, 10% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE