Information on EC 1.1.1.185 - L-glycol dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.185
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RECOMMENDED NAME
GeneOntology No.
L-glycol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
L-glycol:NAD(P)+ oxidoreductase
The 2-hydroxycarbonyl compound formed can be further oxidized to a vicinal dicarbonyl compound. In the reverse direction, vicinal diketones, glyceraldehyde, glyoxal, methylglyoxal, 2-oxo-hydroxyketones and 2-ketoacid esters can be reduced.
CAS REGISTRY NUMBER
COMMENTARY hide
77967-75-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-pentanedione + NADH
L-acetylethylcarbinol + NAD+
show the reaction diagram
diacetyl + NADH
L-acetoin + NAD+
show the reaction diagram
ethyl pyruvate + NAD(P)H
ethyl lactate
show the reaction diagram
methyl pyruvate + NAD(P)H
?
show the reaction diagram
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?
methylglyoxal + NADH
L-lactaldehyde
show the reaction diagram
R1-CO-CHOH-R2 + NAD(P)H
R1-CHOH-CHOH-R2 + NAD(P)+
show the reaction diagram
R1-CO-CO-R2 + NAD(P)H
R1-CO-CHOH-R2 + NAD(P)+
show the reaction diagram
additional information
?
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not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no activity with alpha-NADH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetone
L-acetoin
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probably all L(+)-alpha-hydroxycarbonyls products inhibit uncompetitively at non-saturated concentrations
NAD+
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competitive inhibition for NADH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035 - 10
2,3-Pentanedione
2.013 - 2.59
acetoin
0.109 - 1.5
diacetyl
22
ethyl pyruvate
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0.026 - 0.566
glyceraldehyde
0.053 - 0.522
Glyoxal
0.09 - 90
methylglyoxal
0.006 - 0.0097
NADH
0.0022 - 0.0031
NADPH
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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very stable in both water and low molarity buffers of pH 7 at 0-4°C
286134, 286135
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
easily inactivated in high molarity buffers
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very stable in both water and low molarity buffers of pH 7 at 0-4°C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, enzyme at any stage of purification
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8
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