Information on EC 1.1.1.207 - (-)-menthol dehydrogenase

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The expected taxonomic range for this enzyme is: Mentha

EC NUMBER
COMMENTARY hide
1.1.1.207
-
RECOMMENDED NAME
GeneOntology No.
(-)-menthol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(-)-menthol + NADP+ = (-)-menthone + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
menthol biosynthesis
-
-
Monoterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
(-)-menthol:NADP+ oxidoreductase
Not identical with EC 1.1.1.208 (+)-neomenthol dehydrogenase. Acts also on a number of other cyclohexanols and cyclohexenols.
CAS REGISTRY NUMBER
COMMENTARY hide
81811-46-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-isomenthone + NADPH
(+)-neoisomenthol + NADP+
show the reaction diagram
-
-
-
-
?
(-)-menthone + NADPH
(3R)-menthol + NADP+
show the reaction diagram
(1R)-menthone + NADPH
(1R)-menthol + NADP+
show the reaction diagram
(1R)-menthone + NADPH
(1R)-menthole + NADP+
show the reaction diagram
d-isomenthone + NADPH
d-neoisomenthol + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-isomenthone + NADPH
(+)-neoisomenthol + NADP+
show the reaction diagram
-
-
-
-
?
(-)-menthone + NADPH
(3R)-menthol + NADP+
show the reaction diagram
-
-
-
-
?
(1R)-menthone + NADPH
(1R)-menthole + NADP+
show the reaction diagram
additional information
?
-
-
primary monoterpene biosynthesis pathway, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
-
almost complete inhibition at 0.25 mM
iodoacetamide
-
almost complete inhibition at 0.25 mM
N-ethylmaleimide
-
almost complete inhibition at 0.25 mM
p-hydroxymercuribenzoate
-
almost complete inhibition at 0.05 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.041
(+)-isomenthone
-
pH 7.0, 31C, recombinant enzyme
0.003 - 0.25
(-)-menthone
0.00012 - 0.015
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
(-)-menthone
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
(-)-menthone + NADPH
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8.5
-
50% of maximal activity at pH 4.5 and pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 34070, amino acid sequence calculation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme partially
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcript level of menthol dehydrogenase/menthone reductase is highly upregulated in plants treated with calliterpenone, leading to increased content of menthone and menthol in oil
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
transcript level of menthol dehydrogenase/menthone reductase is highly upregulated in plants treated with calliterpenone, leading to increased content of menthone and menthol in oil