Information on EC 1.1.1.224 - mannose-6-phosphate 6-reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.224
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RECOMMENDED NAME
GeneOntology No.
mannose-6-phosphate 6-reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-mannitol 1-phosphate + NADP+ = D-mannose 6-phosphate + NADPH + H+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannitol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
D-mannitol-1-phosphate:NADP+ 6-oxidoreductase
Involved in the biosynthesis of mannitol in celery (Apium graveolens) leaves.
CAS REGISTRY NUMBER
COMMENTARY hide
88747-79-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
privet
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-
Manually annotated by BRENDA team
no activity in Arabidopsis thaliana
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-
-
Manually annotated by BRENDA team
witchweed
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose 6-phosphate + NADPH
?
show the reaction diagram
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-
-
-
?
D-mannitol 1-phosphate + NADP+
D-mannose 6-phosphate + NADPH + H+
show the reaction diagram
D-mannose 6-phosphate + NADPH
?
show the reaction diagram
D-mannose 6-phosphate + NADPH
D-mannitol 1-phosphate + NADP+
show the reaction diagram
D-mannose 6-phosphate + NADPH + H+
D-mannitol 1-phosphate + NADP+
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannitol 1-phosphate + NADP+
D-mannose 6-phosphate + NADPH + H+
show the reaction diagram
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key enzyme in mannitol production in the parasite
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-
?
D-mannose 6-phosphate + NADPH
?
show the reaction diagram
D-mannose 6-phosphate + NADPH
D-mannitol 1-phosphate + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NaCl
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47% reduction by 100 mM
siRNA
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transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines
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additional information
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overview over substrate and product analogues
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45
D-mannitol 1-phosphate
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-
5.9 - 15.8
D-mannose 6-phosphate
0.0021 - 0.0698
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.008
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crude extract from mature leaves
0.00822
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crude extract from expression in Escherichia coli
0.014
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crude extract from flowering spikes
0.12
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in tubercle
3.76
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purified from leaves
3.93
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purified recombinant enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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D-mannose 6-phosphate
8.5
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D-mannitol 1-phosphate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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inactivated at pH below 6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 45
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75% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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by immunocytochemistry
Manually annotated by BRENDA team
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21-d old, low activity
Manually annotated by BRENDA team
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low activity
Manually annotated by BRENDA team
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by immunocytochemistry
Manually annotated by BRENDA team
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flowering
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
by immunocytochemistry
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
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gel filtration with Sephacryl S-200
33000
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gel filtration with Sephacryl S-200
35000
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theoretical molecular weight, calculated from cDNA sequence
53000
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gel filtration with Superose 12
58000
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gel filtration with Sephacryl S-200
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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-
348002
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
degassed buffers stabilize
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DTT stabilizes
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT stabilizes
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348002
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-21°C, 50% activity retained after 1 month
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4°C, lyophilized active fractions stable for several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; single gene, constitutive, DNA sequence determination and analysis, expression in Escherichia coli
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expression in Arabidopsis thaliana
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expression in transgenic Arabidopsis thaliana plants, the transformed plants show reduced salt injury, less inhibition of vegetative growth, and increased seed production, thus increased salt tolerance, relative to the wild-type plants. When treated with 200 mM NaCl, transformants produce no seeds, but do bolt, and exhibit less chlorosis/necrosis and greater survival and dry weights than the wild-type. Without salt there are no M6PR effects on growth or phenotype, but expression levels of 2272 genes are altered, e.g upregulation of ABA receptor genes, PYL4, PYL5, and PYL6, and downregulation of protein phosphatase 2C genes, ABI1 and ABI2, overview
transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture