Information on EC 1.1.1.240 - N-acetylhexosamine 1-dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.240
-
RECOMMENDED NAME
GeneOntology No.
N-acetylhexosamine 1-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acetyl-alpha-D-glucosamine + NAD+ = N-acetyl-D-glucosaminate + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-acetyl-D-hexosamine:NAD+ 1-oxidoreductase
Also acts on N-acetylgalactosamine and, more slowly, on N-acetylmannosamine. Anomeric specificity was tested with N-acetyl-D-glucosamine, and it was shown that the enzyme is specific for the alpha anomer.
CAS REGISTRY NUMBER
COMMENTARY hide
122785-18-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
No. 53
-
-
Manually annotated by BRENDA team
No. 53
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetyl-D-galactosamine + NAD+
N-acetyl-D-galactoseaminate + NADH
show the reaction diagram
N-acetyl-D-glucosamine + NAD+
N-acetyl-D-glucosaminate + NADH
show the reaction diagram
N-acetyl-D-mannosamine + NAD+ + H2O
N-acetyl-D-mannosaminate + NADH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-D-glucosamine + NAD+
N-acetyl-D-glucosaminate + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
strictly specific for NAD+ as hydrogen acceptor
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Hg2+
-
inhibitory
additional information
-
not effected by Ca2+, Mg2+, Cd2+, Ni2+, Zn2+, Cu2+, Mn2+, Co2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NaN3
-
weak
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
N-acetyl-D-galactosamine
-
-
5.3
N-acetyl-D-glucosamine
-
-
0.24
NAD+
-
with N-acetyl-D-glucosamine as substrate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
932
N-acetyl-D-galactosamine
Pseudomonas sp.
-
-
655
N-acetyl-D-glucosamine
Pseudomonas sp.
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.265
-
crude extract
239
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
SDS-PAGE
124000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10.5
-
most stable
286366
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
10 min, unstable below pH 5 and above pH 12
55
-
10 min, stable
65
-
10 min, 50% loss of activity
70
-
10 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilized against heating by 3 mM NAD+
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, several months, without much loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE