Information on EC 1.1.1.254 - (S)-carnitine 3-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Agrobacterium

EC NUMBER
COMMENTARY hide
1.1.1.254
-
RECOMMENDED NAME
GeneOntology No.
(S)-carnitine 3-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-carnitine + NAD+ = 3-dehydrocarnitine + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-carnitine degradation I
-
-
carnitine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-carnitine:NAD+ oxidoreductase
Specific for the (S)-enantiomer of carnitine, i.e., the enantiomer of the substrate of EC 1.1.1.108 carnitine 3-dehydrogenase
CAS REGISTRY NUMBER
COMMENTARY hide
169277-49-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-carnitine + NAD+
3-dehydrocarnitine + NADH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-carnitine + NAD+
3-dehydrocarnitine + NADH + H+
show the reaction diagram
-
substrate is derived as a waste product from chemical production procedures of L(-)-carnitine
formation of poly-beta-hydroxybutyrate as a storage product
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
1 mM, increases activity up to 31%
Ni2+
-
5 mM, increases activity up to 31%
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Carnitine amide
-
competitive inhibitor of (S)-carnitine oxidation
choline
-
competitive inhibitor of (S)-carnitine oxidation
crotonobetaine
-
competitive inhibitor of (S)-carnitine oxidation
gamma-butyrobetaine
-
competitive inhibitor of (S)-carnitine oxidation
glycine betaine
-
competitive inhibitor of (S)-carnitine oxidation
Hexadecyltrimethylammonium bromide
-
above 5 mM, 90% loss of activity
L(-)-carnitine
-
competitive inhibitor of (S)-carnitine oxidation
p-chloromercuribenzoate
-
-
SDS
-
above 5 mM, 90% loss of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.5
(S)-carnitine
-
-
1.2
3-dehydrocarnitine
-
-
0.15
NAD+
-
-
0.047
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
-
3-dehydrocarnitine + NADH
9 - 9.5
-
(S)-carnitine + NAD+
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
organism is able to grow on synthetic D(+)-carnitine, but not on physiological L(-)-carnitine, as sole carbon and nitrogen source
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
-
3 * 28000, SDS-PAGE, laser-induced mass spectrometry
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
4°C, stable for at least 3 days
9588
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
rapid decrease of stability above
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, pH 6-8, stable for at least 3 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
Agrobacterium radiobacter can be used for conversion of the waste product L(-)-carnitine to poly-beta-hydroxybutyrate valuable as a biodegradable and biocompatible plastic