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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
GDP-4-keto-6-deoxy-D-mannose + NADPH + H+
GDP-L-fucose + NADP+
additional information
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fucosylation is regulated by complicated mechanisms that involve several factors: fucosyltransferases, GDP-fucose transporter, and GDP-fucose-synthetic enzymes, such as GDP-mannose 4,6-dehydratase, GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase, and GDP-fucose pyrophosphorylase, fucose metabolism, overview
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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4
product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy
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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
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the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4
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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
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GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4
product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
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the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
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the enzyme is required for the formation of activated L-fucose
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GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
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GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
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GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
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GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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bifunctional enzyme that exhibits epimerase and reductase activity: 3,5-epimerization to GDP-4-keto-6-deoxy-L-galactose and subsequent NADPH-dependent reduction to GDP-L-fucose
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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catalyzes the last two steps in the de novo synthesis of L-fucose
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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catalyzes the stereospecific hydride transfer of the ProS hydrogen from NADPH to carbon 4 of the mannose sugar
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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enzyme also utilizes NADH but with lower affinity
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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enzyme uses NADH as cofactor
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GDP-4-keto-6-deoxy-D-mannose + NADPH + H+
GDP-L-fucose + NADP+
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GDP-4-keto-6-deoxy-D-mannose + NADPH + H+
GDP-L-fucose + NADP+
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Breast Neoplasms
Oncogenic potential of TSTA3 in breast cancer and its regulation by the tumor suppressors miR-125a-5p and miR-125b.
Breast Neoplasms
Pathway-based classification of cancer subtypes.
Breast Neoplasms
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Carcinogenesis
Oncogenic potential of TSTA3 in breast cancer and its regulation by the tumor suppressors miR-125a-5p and miR-125b.
Carcinogenesis
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Carcinoma
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Carcinoma
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Carcinoma
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Carcinoma, Hepatocellular
An Alkynyl-Fucose Halts Hepatoma Cell Migration and Invasion by Inhibiting GDP-Fucose-Synthesizing Enzyme FX, TSTA3.
Carcinoma, Hepatocellular
Expression of genes that control core fucosylation in hepatocellular carcinoma: Systematic review.
Carcinoma, Hepatocellular
Tissue-specific transplantation antigen P35B (TSTA3) immune response-mediated metabolism coupling cell cycle to postreplication repair network in no-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) by biocomputation.
Esophageal Squamous Cell Carcinoma
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Esophageal Squamous Cell Carcinoma
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Esophageal Squamous Cell Carcinoma
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Insulin Resistance
Significant associations among hemostatic parameters, adipokines, and components of the metabolic syndrome in Japanese preschool children.
Lung Neoplasms
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Lymphatic Metastasis
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Multiple Sclerosis
GDP-l-fucose synthase is a CD4+ T cell-specific autoantigen in DRB3*02:02 patients with multiple sclerosis.
Neoplasm Metastasis
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Neoplasm Metastasis
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Neoplasms
Expression of genes that control core fucosylation in hepatocellular carcinoma: Systematic review.
Neoplasms
Oncogenic potential of TSTA3 in breast cancer and its regulation by the tumor suppressors miR-125a-5p and miR-125b.
Neoplasms
Pathway-based classification of cancer subtypes.
Neoplasms
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Osteonecrosis
ARG2, MAP4K5 and TSTA3 as Diagnostic Markers of Steroid-Induced Osteonecrosis of the Femoral Head and Their Correlation With Immune Infiltration.
Tuberculosis
Deciphering the genetic bases of the structural diversity of phenolic glycolipids in strains of the Mycobacterium tuberculosis complex.
Virus Diseases
Paramecium bursaria Chlorella virus 1 encodes two enzymes involved in the biosynthesis of GDP-L-fucose and GDP-D-rhamnose.
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Bonin, C.P.; Reiter, W.D.
A bifunctional epimerase-reductase acts downstream of the MUR1 gene product and completes the de novo synthesis of GDP-L-fucose in Arabidopsis
Plant J.
21
445-454
2000
Arabidopsis thaliana
brenda
Chang, S.; Duerr, B.; Serif, G.
An epimerase-reductase in L-fucose synthesis
J. Biol. Chem.
263
1693-1697
1988
Sus scrofa
brenda
Jarvinen, N.; Maki, M.; Rabina, J.; Roos, C.; Mattila, P.; Renkonen, R.
Cloning and expression of Helicobacter pylori GDP-L-fucose synthesizing enzymes (GMD and GMER) in Saccharomyces cerevisiae
Eur. J. Biochem.
268
6458-6464
2001
Helicobacter pylori, Helicobacter pylori NTC 11637
brenda
Mattila, P.; Rabina, J.; Hortling, S.; Helin, J.; Renkonen, R.
Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae
Glycobiology
10
1041-1047
2000
Escherichia coli
brenda
Menon, S.; Stahl, M.; Kumar, R.; Xu, G.Y.; Sullivan, F.
Stereochemical course and steady state mechanism of the reaction catalyzed by the GDP-fucose synthetase from Escherichia coli
J. Biol. Chem.
274
26743-26750
1999
Escherichia coli
brenda
Ohyama, C.; Smith, P.L.; Angata, K.; Fukuda, M.N.; Lowe, J.B.; Fukuda, M.
Molecular cloning and expression of GDP-D-mannose-4,6-dehydratase, a key enzyme for fucose metabolism defective in Lec13 cells
J. Biol. Chem.
273
14582-14587
1998
Homo sapiens
brenda
Rizzi, M.; Tonetti, M.; Vigevani, P.; Sturla, L.; Bisso, A.; De Flora, A.; Bordo, D.; Bolognesi, M.
GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from Escherichia coli, a key enzyme in the biosynthesis of GDP-L-fucose, displays the structural characteristics of the RED protein homology superfamily
Structure
6
1453-1465
1998
Escherichia coli
brenda
Rosano, C.; Bisso, A.; Izzo, G.; Tonetti, M.; Sturla, L.; De Flora, A.; Bolognesi, M.
Probing the catalytic mechanism of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase by kinetic and crystallographic characterization of site-specific mutants
J. Mol. Biol.
303
77-91
2000
Escherichia coli
brenda
Wu, B.; Zhang, Y.; Wang, P.G.
Identification and characterization of GDP-D-mannose 4,6-dehydratase and GDP-L-fucose synthetase in a GDP-L-fucose biosynthetic gene cluster from Helicobacter pylori
Biochem. Biophys. Res. Commun.
285
364-371
2001
Helicobacter pylori
brenda
Niittymaki, J.; Mattila, P.; Renkonen, R.
Differential gene expression of GDP-L-fucose-synthesizing enzymes, GDP-fucose transporter and fucosyltransferase VII
APMIS
114
539-548
2006
Mus musculus, Rattus norvegicus
brenda
Rhomberg, S.; Fuchsluger, C.; Rendi?, D.; Paschinger, K.; Jantsch, V.; Kosma, P.; Wilson, I.B.
Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster
FEBS J.
273
2244-2256
2006
Caenorhabditis elegans (G5EER4), Caenorhabditis elegans, Drosophila melanogaster (Q9W1X8), Drosophila melanogaster
brenda
Moriwaki, K.; Noda, K.; Nakagawa, T.; Asahi, M.; Yoshihara, H.; Taniguchi, N.; Hayashi, N.; Miyoshi, E.
A high expression of GDP-fucose transporter in hepatocellular carcinoma is a key factor for increases in fucosylation
Glycobiology
17
1311-1320
2007
Homo sapiens
brenda
Byun, S.G.; Kim, M.D.; Lee, W.H.; Lee, K.J.; Han, N.S.; Seo, J.H.
Production of GDP-L-fucose, L-fucose donor for fucosyloligosaccharide synthesis, in recombinant Escherichia coli
Appl. Microbiol. Biotechnol.
74
768-775
2007
Escherichia coli K-12
brenda
Malaga, W.; Constant, P.; Euphrasie, D.; Cataldi, A.; Daffe, M.; Reyrat, J.M.; Guilhot, C.
Deciphering the genetic bases of the structural diversity of phenolic glycolipids in strains of the Mycobacterium tuberculosis complex
J. Biol. Chem.
283
15177-15184
2008
Mycobacterium tuberculosis, no activity in Mycobacterium bovis
brenda
Lau, S.T.; Tanner, M.E.
Mechanism and active site residues of GDP-fucose synthase
J. Am. Chem. Soc.
130
17593-17602
2008
Escherichia coli K-12, Escherichia coli (P32055)
brenda
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (O49213), Arabidopsis thaliana (Q9LMU0)
brenda
Ren, Y.; Perepelov, A.V.; Wang, H.; Zhang, H.; Knirel, Y.A.; Wang, L.; Chen, W.
Biochemical characterization of GDP-L-fucose de novo synthesis pathway in fungus Mortierella alpina
Biochem. Biophys. Res. Commun.
391
1663-1669
2010
Mortierella alpina (D3JU54), Mortierella alpina
brenda
Zhou, H.; Sun, L.; Li, J.; Xu, C.; Yu, F.; Liu, Y.; Ji, C.; He, J.
The crystal structure of human GDP-L-fucose synthase
Acta Biochim. Biophys. Sin. (Shanghai)
45
720-725
2013
Homo sapiens
brenda
Sanz, S.; Bandini, G.; Ospina, D.; Bernabeu, M.; Marino, K.; Fernandez-Becerra, C.; Izquierdo, L.
Biosynthesis of GDP-fucose and other sugar nucleotides in the blood stages of Plasmodium falciparum
J. Biol. Chem.
288
16506-16517
2013
Plasmodium falciparum (A0A143ZXB3), Plasmodium falciparum
brenda
Kizuka, Y.; Nakano, M.; Yamaguchi, Y.; Nakajima, K.; Oka, R.; Sato, K.; Ren, C.T.; Hsu, T.L.; Wong, C.H.; Taniguchi, N.
An alkynyl-fucose halts hepatoma cell migration and invasion by inhibiting GDP-fucose-synthesizing enzyme FX, TSTA3
Cell Chem. Biol.
24
1467-1478
2017
Homo sapiens (Q13630)
brenda
Li, L.; Kim, S.; Heo, J.; Kim, T.; Seo, J.; Han, N.
One-pot synthesis of GDP-L-fucose by a four-enzyme cascade expressed in Lactococcus lactis
J. Biotechnol.
264
1-7
2017
Escherichia coli (P32055), Escherichia coli K12 (P32055)
brenda
Planas, R.; Santos, R.; Tomas-Ojer, P.; Cruciani, C.; Lutterotti, A.; Faigle, W.; Schaeren-Wiemers, N.; Espejo, C.; Eixarch, H.; Pinilla, C.; Martin, R.; Sospedra, M.
GDP-L-fucose synthase is a CD4+ T cell-specific autoantigen in DRB3*02 02 patients with multiple sclerosis
Sci. Transl. Med.
10
eaat4301
2018
Homo sapiens (Q13630), Homo sapiens
brenda