Information on EC 1.1.1.273 - vellosimine dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.273
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RECOMMENDED NAME
GeneOntology No.
vellosimine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10-deoxysarpagine + NADP+ = vellosimine + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
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Indole alkaloid biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
10-deoxysarpagine:NADP+ oxidoreductase
Also acts on related alkaloids with an endo-aldehyde group as vellosimine (same stereochemistry at C-16) but only slight activity with exo-aldehydes. Detected in many cell suspension cultures of plants from the family Apocynaceae.
CAS REGISTRY NUMBER
COMMENTARY hide
86777-26-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Alstonia scholaris
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Manually annotated by BRENDA team
no activity in Carissa macrocarpa
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Manually annotated by BRENDA team
no activity in Catharanthus roseus
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Manually annotated by BRENDA team
no activity in Coleus blumei
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Manually annotated by BRENDA team
no activity in Malus sylvestris
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Manually annotated by BRENDA team
no activity in Nicotiana sylvestris
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Manually annotated by BRENDA team
no activity in Picralima nitida
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Manually annotated by BRENDA team
no activity in Rhazya stricta
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Manually annotated by BRENDA team
no activity in Stemmadenia tomentosa
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Manually annotated by BRENDA team
no activity in Tabernanthe iboga
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Manually annotated by BRENDA team
no activity in Voacanga africana
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Manually annotated by BRENDA team
Vinca herbacea
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
16-epi-vellosimine + NADPH
?
show the reaction diagram
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-
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?
deoxyajmalal B + NADPH
?
show the reaction diagram
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?
gardneral + NADPH
?
show the reaction diagram
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-
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?
geissoschizal + NADPH
?
show the reaction diagram
N-methylgardneral + NADPH
?
show the reaction diagram
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?
vellosimine + NADPH
10-deoxysarpagine + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
vellosimine + NADPH
10-deoxysarpagine + NADP+
show the reaction diagram
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specific for NADPH, may be involved in the conversion of sarpagine type alkaloids
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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1 mM, 71% inhibition
EDTA
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1 mM, 77% inhibition
NADP+
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noncompetitive inhibition
neocuproin
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1 mM, 65% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
geissoschizal
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0.0167
vellosimine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00018
Vinca herbacea
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vellosimine reduction in cell suspension culture
0.0006
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vellosimine reduction in cell suspension culture
0.00096
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geissoschizal reduction
0.0012
0.013
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vellosimine reduction in cell suspension culture
0.0156
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vellosimine reduction in cell suspension culture
0.0192
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vellosimine reduction in cell suspension culture
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
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50% of maximal activity at pH 6.8 and pH 7.6 respectively
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
highly instable enzyme, in crude extracts, half-life of activity is 25 h at 4°C, 0.25% activity remains after 7 d, activity depends completely on the presence of 2-mercaptoethanol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-cellulose, hydroxylapatite, Matrex gel Green A
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