Information on EC 1.1.1.302 - 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.302
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RECOMMENDED NAME
GeneOntology No.
2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NAD(P)+ = 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NAD(P)H + H+
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
flavin biosynthesis II (archaea)
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flavin biosynthesis III (fungi)
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flavin biosynthesis
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Riboflavin metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one:NAD(P)+ oxidoreductase
The reaction proceeds in the opposite direction. A step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant. Differs from EC 1.1.1.193 [5-amino-6-(5-phosphoribosylamino)uracil reductase] since it does not catalyse the reduction of 5-amino-6-ribosylaminopyrimidine-2,4(1H,3H)-dione 5'-phosphate [1].
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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key enzymes for xylitol production in yeasts are xylose reductase and xylitol dehydrogenase, EC 1.1.1.9, overview
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5-diamino-6-(1-D-ribitylamino)-4(3H)-pyrimidinone 5'-phosphate + NAD+
2,5-diamino-6-(1-D-ribitylamino)-4(3H)-pyrimidinone 5'-phosphate + NAD+
show the reaction diagram
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?
2,5-diamino-6-(1-D-ribosylamino)-4(3H)-pyrimidinone 5'-phosphate + NADH + H+
2,5-diamino-6-(1-D-ribitylamino)-4(3H)-pyrimidinone 5'-phosphate + NAD+
show the reaction diagram
2,5-diamino-6-(1-D-ribosylamino)-4(3H)-pyrimidinone 5'-phosphate + NADPH + H+
2,5-diamino-6-(1-D-ribitylamino)-4(3H)-pyrimidinone 5'-phosphate + NADP+
show the reaction diagram
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+
2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
show the reaction diagram
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + NADH + H+
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate + NAD+
show the reaction diagram
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step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant
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?
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + NADPH + H+
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate + NADP+
show the reaction diagram
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step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant
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?
additional information
?
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the enzyme does not catalyze the pyrimidine nucleotide-dependent reduction of 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,5-diamino-6-(1-D-ribosylamino)-4(3H)-pyrimidinone 5'-phosphate + NADH + H+
2,5-diamino-6-(1-D-ribitylamino)-4(3H)-pyrimidinone 5'-phosphate + NAD+
show the reaction diagram
-
step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant
-
-
?
2,5-diamino-6-(1-D-ribosylamino)-4(3H)-pyrimidinone 5'-phosphate + NADPH + H+
2,5-diamino-6-(1-D-ribitylamino)-4(3H)-pyrimidinone 5'-phosphate + NADP+
show the reaction diagram
-
step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant
-
-
?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+
2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
show the reaction diagram
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + NADH + H+
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate + NAD+
show the reaction diagram
-
step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant
-
-
?
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + NADPH + H+
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate + NADP+
show the reaction diagram
-
step in riboflavin biosynthesis, NADPH and NADH function equally well as reductant
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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analytical ultracentrifugation, boundary sedimentation
50600
sedimentation equilibrium analysis
57800
sedimentation equilibrium analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapour diffusion method. The structure of the enzyme in complex with the cofactor nicotinamide adenine dinucleotide phosphate is determined by X-ray crystallography at a resolution of 2.5 A
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli using a synthetic gene, the synthetic open reading frame is optimized for expression in Escherichia coli. The hypothetical open reading frame MJ0671 of Methanocaldococcus jannaschii predicts a protein of 224 amino acid residues. Because MJ0671 contains numerous codons that are poorly translated in Escherichia coli, a synthetic gene is designed that is optimized for the Escherichia coli codon usage. Approximately 31% (69 of 224) of the codons are replaced, and 14 singular restriction sites are introduced. The DNA sequence is assembled from 16 synthetic oligonucleotides by a sequence of eight PCR steps. The synthetic gene is transcribed efficiently in a recombinant Escherichia coli strain, affording approximately 30% of cellular protein
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expression in Escherichia coli
overexpressed in Escherichia coli
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Show AA Sequence (101 entries)
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