Information on EC 1.1.1.313 - sulfoacetaldehyde reductase

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The expected taxonomic range for this enzyme is: Chromohalobacter salexigens

EC NUMBER
COMMENTARY hide
1.1.1.313
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RECOMMENDED NAME
GeneOntology No.
sulfoacetaldehyde reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
isethionate + NADP+ = 2-sulfoacetaldehyde + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfoacetaldehyde degradation III
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Taurine and hypotaurine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
isethionate:NADP+ oxidoreductase
Catalyses the reaction only in the opposite direction. Involved in taurine degradation. The bacterium Chromohalobacter salexigens strain DSM 3043 possesses two enzymes that catalyse this reaction, a constitutive enzyme (encoded by isfD2) and an inducible enzyme (encoded by isfD). The latter is induced by taurine, and is responsible for most of the activity observed in taurine-grown cells.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-sulfoacetaldehyde + NADPH + H+
isethionate + NADP+
show the reaction diagram
4-oxobutyrate + NADPH + H+
?
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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highly specific for NADPH. NADH is not a substrate.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-oxobutyrate
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90% substrate inhibition at 20 mM
additional information
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not inhibited by formaldehyde, acetaldehyde, betaine aldehyde, propionaldehyde, DL-glyceraldehyde, phosphonoacetaldehyde, glyoxylate, 2-oxobutyrate, 4-oxobutyrate and 3-sulfopropanaldehyde
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
2-sulfoacetaldehyde
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in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23C
0.061
NADPH
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in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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in Tris/H2SO4 buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
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IsfD shows a broad pH optimum from about pH 6.5 to pH 9.5. There is a steep increase in activity from pH 4.5 (negligible activity) and an equally steep decrease above pH 9.5
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27100
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estimated from amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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IsfD is stable in Tris/H2SO4 buffer, pH 9.0
709846
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Mono Q column chromatography, phenyl Superose gel filtration
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