Information on EC 1.1.1.338 - (2R)-3-sulfolactate dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Chromohalobacter salexigens

EC NUMBER
COMMENTARY hide
1.1.1.338
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RECOMMENDED NAME
GeneOntology No.
(2R)-3-sulfolactate dehydrogenase (NADP+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2R)-3-sulfolactate + NADP+ = 3-sulfopyruvate + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfolactate degradation I
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Cysteine and methionine metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(2R)-3-sulfolactate:NADP+ oxidoreductase
The enzyme from the bacterium Chromohalobacter salexigens can only utilize NADP+. It functions both biosynthetically in coenzyme M biosynthesis and degradatively, in the degradation of sulfolactate. It can not use (S)-malate and (S)-lactate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
DSM 3043
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-3-sulfolactate + NADP+
3-sulfopyruvate + NADPH + H+
show the reaction diagram
specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate
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r
3-sulfopyruvate + NADPH + H+
(S)-3-sulfolactate + NADP+
show the reaction diagram
specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate
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r
additional information
?
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no substrate: pyruvate, oxaloacetate, (S)-malate, (S)-lactate, (R)-malate, (R)-lactate
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
no cosubstrate: NADH
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19
(S)-3-sulfolactate
pH 9.0, 23C
0.14
3-sulfopyruvate
pH 9.0, 23C
1.2
NADP+
pH 9.0, 23C
0.1
NADPH
pH 9.0, 23C
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
2 * 37000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18C, stable against freezing and thawing
4C, stable for several weeks
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced by growth on sulfolactate