Information on EC 1.1.1.356 - GDP-L-colitose synthase

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The expected taxonomic range for this enzyme is: Yersinia pseudotuberculosis

EC NUMBER
COMMENTARY hide
1.1.1.356
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RECOMMENDED NAME
GeneOntology No.
GDP-L-colitose synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-colitose + NAD(P)+ = GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
GDP-L-colitose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-colitose:NAD(P)+ 4-oxidoreductase (5-epimerizing)
The enzyme is involved in biosynthesis of L-colitose, a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides, where it catalyses the reaction in the reverse direction. The enzyme also performs the NAD(P)H-dependent epimerisation at C-5 of the sugar. The enzyme from Yersinia pseudotuberculosis is Si-specific with respect to NAD(P)H [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+
GDP-beta-L-colitose + NAD(P)+
show the reaction diagram
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-colitose + NAD+
show the reaction diagram
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-colitose + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+
GDP-beta-L-colitose + NAD(P)+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075 - 0.139
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, stable for at least 6 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-terminal His6-tagged ColC
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE