Information on EC 1.1.1.357 - 3alpha-hydroxysteroid 3-dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.357
-
RECOMMENDED NAME
GeneOntology No.
3alpha-hydroxysteroid 3-dehydrogenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
brassinosteroid biosynthesis I
-
-
brassinosteroid biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
3alpha-hydroxysteroid:NAD(P)+ 3-oxidoreductase
The enzyme acts on multiple 3alpha-hydroxysteroids, such as androsterone and 5 alpha-dihydrotestosterone. The mammalian enzymes are involved in inactivation of steroid hormones, while the bacterial enzymes are involved in steroid degradation. This entry stands for enzymes whose stereo-specificity with respect to NAD+ or NADP+ is not known. [cf. EC 1.1.1.50, 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) and EC 1.1.1.213, 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Boehringer patent strain
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-tetralol + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
(S)-1,2,3,4-tetrahydronaphth-1-ol + NADP+
1,2,3,4-tetrahydronaphth-1-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
(S)-indan-1-ol + NADP+
indan-1-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
(S)-tetralol + NADP+
1-tetralone + NADPH + H+
show the reaction diagram
-
-
-
-
?
1-acenaphthenol + NADP+
acenaphthylen-1(2H)-one + NADPH + H+
show the reaction diagram
-
-
-
?
17beta-estradiol + NAD(P)H + H+
estrone + NAD(P)+
show the reaction diagram
-
-
-
-
?
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+
2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+
show the reaction diagram
20alpha-hydroxyprogesterone + NAD(P)H + H+
progesterone + NAD(P)+
show the reaction diagram
-
-
-
-
?
3-dehydro-alpha-ecdysone + NAD(P)H + H+
alpha-ecdysone + NAD(P)+
show the reaction diagram
3-dehydro-beta-ecdysone + NAD(P)H + H+
beta-ecdysone + NAD(P)+
show the reaction diagram
3alpha-androstanediol + NAD(P)+
5alpha-dihydrotestosterone + NAD(P)H + H+
show the reaction diagram
-
-
-
-
r
3alpha-androstanediol + NAD+
5alpha-dihydrotestosterone + NADH + H+
show the reaction diagram
-
-
-
-
?
3alpha-hydroxydesogestrel + NAD(P)+
3-oxodesogestrel + NAD(P)H + H+
show the reaction diagram
4-nitroacetophenone + NAD(P)H + H+
1-(4-nitrophenyl)ethanol + NAD(P)+
show the reaction diagram
4-nitrobenzaldehyde + NAD(P)H + H+
(4-nitrophenyl)methanol + NAD(P)+
show the reaction diagram
5alpha-androstan-3alpha-ol-17-one + NADP+
(5alpha)-androstan-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
-
?
5alpha-androstane-3,17-dione + NAD(P)H + H+
androsterone + NAD(P)+
show the reaction diagram
-
-
-
-
?
5alpha-androstane-3alpha,17beta-diol + NADP+
(5alpha)-androstan-17-beta-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
5alpha-dihydroprogesterone + NADPH + H+
allopregnanolone + 5alpha,20alpha-tetrahydroprogesterone + NADP+
show the reaction diagram
-
-
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
(5alpha,17beta)-androstane-3,17-diol + NAD(P)+
show the reaction diagram
5alpha-dihydrotestosterone + NAD(P)H + H+
3alpha-androstanediol + NAD(P)+
show the reaction diagram
5alpha-dihydrotestosterone + NADPH + H+
3alpha-androstanediol + NADP+
show the reaction diagram
-
-
-
-
?
5alpha-dihydrotestosterone + NADPH + H+
5alpha-androstane-3alpha,17beta-diol + NADP+
show the reaction diagram
-
-
-
r
5beta-androstan-3alpha-ol-17-one + NADP+
(5beta)-androstan-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
-
?
5beta-androstane-alpha,17beta-diol + NADP+
(5beta)-androstan-17-beta-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
5beta-dihydroprogesterone + NADPH + H+
pregnanolone + NADP+
show the reaction diagram
-
-
-
r
5beta-dihydrotestosterone + NAD(P)H + H+
(5beta,17beta)-androstane-3,17-diol + NAD(P)+
show the reaction diagram
5beta-pregnane-3alpha,20alpha-diol + NADP+
(5beta)-pregnan-20alpha-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
7alpha,12alpha-dihydroxy-5beta-cholestan-3-one + NAD(P)+
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestane + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
9alpha,11beta-prostaglandin F2 + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
a 3-alpha-hydroxysteroid + NAD(P)+
a 3-oxosteroid + NAD(P)H + H+
show the reaction diagram
-
-
-
?
a 3alpha-hydroxysteroid + NAD(P)+
a 3-oxosteroid + NAD(P)H + H+
show the reaction diagram
allopregnanolone + 5alpha,20alpha-tetrahydroprogesterone + NADP+
5alpha-dihydroprogesterone + NADPH + H+
show the reaction diagram
-
-
-
r
androstandiol + NAD(P)+
(5alpha,17beta)-17-hydroxyandrostan-3-one + NAD(P)H + H+
show the reaction diagram
-
-
-
?
androstandione + NAD(P)H + H+
3-hydroxyandrostan-17-one + NAD(P)+
show the reaction diagram
-
-
-
?
androsterone + NAD(P)+
(5alpha)-androstane-3,17-dione + NAD(P)H + H+
show the reaction diagram
androsterone + NAD(P)+
5alpha-androstane-3,17-dione + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
androsterone + NAD+
(5alpha)-androstane-3,17-dione + NADH + H+
show the reaction diagram
-
-
-
-
?
androsterone + NADP+
(5alpha)-androstane-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
-
?
androsterone + NADP+
5alpha-androstane-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
?
chenodeoxycholic acid + NADP+
(5beta,7alpha,8xi)-7-hydroxy-3-oxocholan-24-oic acid + NADPH + H+
show the reaction diagram
-
-
-
?
chenodeoxycholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
cholic acid + NAD(P)+
(5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+
show the reaction diagram
cholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
deoxycholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
dihydrotestosterone + NAD(P)H + H+
5alpha-androstane-3alpha,17beta-diol + NAD(P)+
show the reaction diagram
-
-
-
-
?
epiandrosterone + NAD(P)+
(5alpha)-androstane-3,17-dione + NAD(P)H + H+
show the reaction diagram
-
-
-
?
estrone + NAD(P)H + H+
17beta-estradiol + NAD(P)+
show the reaction diagram
-
-
-
-
?
fusidic acid + NAD(P)+
(2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+
show the reaction diagram
fusidic acid + NADP+
(2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NADPH + H+
show the reaction diagram
lithocholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
metyrapol + NAD(P)+
2-methyl-1,2-di(pyridin-3-yl)propan-1-one + NAD(P)H + H+
show the reaction diagram
-
-
-
?
metyrapone + NAD(P)H + H+
2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+
show the reaction diagram
progesterone + NAD(P)H + H+
20alpha-hydroxyprogesterone + NAD(P)+
show the reaction diagram
-
-
-
-
?
testosterone + NAD(P)H + H+
DELTA4-androstene-3,17-dione + NAD(P)+
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-tetralol + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
(S)-indan-1-ol + NADP+
indan-1-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
(S)-tetralol + NADP+
1-tetralone + NADPH + H+
show the reaction diagram
-
-
-
-
?
17beta-estradiol + NAD(P)H + H+
estrone + NAD(P)+
show the reaction diagram
-
-
-
-
?
20alpha-hydroxyprogesterone + NAD(P)H + H+
progesterone + NAD(P)+
show the reaction diagram
-
-
-
-
?
3alpha-androstanediol + NAD(P)+
5alpha-dihydrotestosterone + NAD(P)H + H+
show the reaction diagram
-
-
-
-
r
5alpha-androstane-3,17-dione + NAD(P)H + H+
androsterone + NAD(P)+
show the reaction diagram
-
-
-
-
?
5alpha-androstane-3alpha,17beta-diol + NADP+
(5alpha)-androstan-17-beta-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
5alpha-dihydroprogesterone + NADPH + H+
allopregnanolone + 5alpha,20alpha-tetrahydroprogesterone + NADP+
show the reaction diagram
P52895
-
-
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
3alpha-androstanediol + NAD(P)+
show the reaction diagram
5beta-androstan-3alpha-ol-17-one + NADP+
(5beta)-androstan-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
-
?
5beta-androstane-alpha,17beta-diol + NADP+
(5beta)-androstan-17-beta-ol-3-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
5beta-dihydroprogesterone + NADPH + H+
pregnanolone + NADP+
show the reaction diagram
P52895
-
-
-
r
7alpha,12alpha-dihydroxy-5beta-cholestan-3-one + NAD(P)+
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestane + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
a 3alpha-hydroxysteroid + NAD(P)+
a 3-oxosteroid + NAD(P)H + H+
show the reaction diagram
allopregnanolone + 5alpha,20alpha-tetrahydroprogesterone + NADP+
5alpha-dihydroprogesterone + NADPH + H+
show the reaction diagram
P52895
-
-
-
r
androsterone + NAD(P)+
5alpha-androstane-3,17-dione + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
androsterone + NAD+
(5alpha)-androstane-3,17-dione + NADH + H+
show the reaction diagram
-
-
-
-
?
androsterone + NADP+
(5alpha)-androstane-3,17-dione + NADPH + H+
show the reaction diagram
-
-
-
-
?
chenodeoxycholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
cholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
deoxycholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
estrone + NAD(P)H + H+
17beta-estradiol + NAD(P)+
show the reaction diagram
-
-
-
-
?
lithocholic acid + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
-
?
progesterone + NAD(P)H + H+
20alpha-hydroxyprogesterone + NAD(P)+
show the reaction diagram
-
-
-
-
?
testosterone + NAD(P)H + H+
DELTA4-androstene-3,17-dione + NAD(P)+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
isoform AKR1C2 functions preferentially as a 3-ketosteroid reductase
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
-
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17beta-estradiol
-
competitive inhibitor
3,5,3',5'-tetraiodothyropropionic acid
-
competitive inhibitor
chenodeoxycholic acid
-
-
Flufenamic acid
Hexestrol
-
-
Ibuprofen
-
-
indomethacin
-
-
lithocholic acid
-
-
Medroxyprogesterone acetate
-
-
Phenolphthalein
ursodeoxycholate
-
micromolar inhibitor, competitive inhibitor versus 5alpha-dihydroprogesterone and uncompetitive inhibitor versus NADPH
Ursodeoxycholic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-ibuprofen
-
167% activity at 1 mM
Clofibric acid
-
240% activity at 0.1 mM
D-thyroxine
-
260% activity at 1 mM
L-thyroxine
-
237% activity at 1 mM
Sulfobromophthalein
-
328% activity at 0.005 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16 - 0.68
(R)-tetralol
0.14 - 0.26
(S)-1,2,3,4-tetrahydronaphth-1-ol
0.146 - 0.52
(S)-indan-1-ol
0.11 - 0.29
(S)-tetralol
1
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone
-
pH 7.0, temperature not specified in the publication, NAD(P)H
0.025 - 0.028
3-dehydro-alpha-ecdysone
0.035
3-dehydro-beta-ecdysone
-
pH 7.0, temperature not specified in the publication, NAD(P)H
0.0105
3alpha-androstanediol
-
isoform AKR1C4, at pH 7.4 and 25C
0.0152 - 0.0216
3alpha-hydroxydesogestrel
0.9 - 1
4-Nitroacetophenone
1 - 1.09
4-Nitrobenzaldehyde
0.002 - 0.0022
5alpha-Androstan-3alpha-ol-17-one
0.00144
5alpha-androstane-3,17-dione
-
isoform AKR1C4, at pH 7.4 and 25C
0.0008 - 0.0031
5alpha-androstane-3alpha,17beta-diol
0.0018
5alpha-dihydroprogesterone
-
in 100 mM potassium phosphate, pH 7.0, at 25C
0.0012 - 0.0192
5alpha-dihydrotestosterone
0.0009 - 0.0018
5beta-Androstan-3alpha-ol-17-one
0.0012 - 0.0022
5beta-androstane-3alpha,17beta-diol
0.0111 - 0.0201
5beta-dihydrotestosterone
0.001
5beta-pregnane-3alpha,20alpha-diol
-
native enzyme, at pH 7.4 and 25C; recombinant enzyme, at pH 7.4 and 25C
0.208 - 0.22
9alpha,11beta-prostaglandin F2
0.0015
allopregnanolone
-
in 100 mM potassium phosphate, pH 7.0, at 25C
0.0328
androstandiol
-
pH 8.9, temperature not specified in the publication, NAD(P)+
0.0357
androstandione
-
pH 7.0, temperature not specified in the publication, NAD(P)H
0.0005 - 0.0267
androsterone
0.0011 - 0.0029
chenodeoxycholic acid
0.025 - 0.059
cholic acid
0.0013 - 0.0097
deoxycholic acid
0.0011
dihydrotestosterone
-
pH 7.5, 37C
0.0207
epiandrosterone
-
pH 8.9, temperature not specified in the publication, NAD(P)+
0.003 - 0.0068
Fusidic acid
0.001 - 0.0019
lithocholic acid
2.1
metyrapol
-
pH 8.9, temperature not specified in the publication, NAD(P)+
0.61 - 0.63
Metyrapone
1 - 1.2
NAD+
0.00023 - 0.0017
NADP+
0.00007
NADPH
-
in 100 mM potassium phosphate, pH 7.0, at 25C
additional information
additional information
-
Km-value for the 17beta-oxidation of testosterone is 0.00067 mM, and Km-value for the 17beta-reduction of androstenedione is 0.00138 mM
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 0.77
(R)-tetralol
0.2 - 0.27
(S)-1,2,3,4-tetrahydronaphth-1-ol
0.1 - 0.4
(S)-indan-1-ol
0.2 - 0.43
(S)-tetralol
0.035
3alpha-androstanediol
Homo sapiens
-
isoform AKR1C4, at pH 7.4 and 25C
0.007
5alpha-Androstan-3alpha-ol-17-one
Homo sapiens
-
native enzyme, at pH 7.4 and 25C; recombinant enzyme, at pH 7.4 and 25C
0.03
5alpha-androstane-3,17-dione
Homo sapiens
-
isoform AKR1C4, at pH 7.4 and 25C
0.087 - 0.35
5alpha-androstane-3alpha,17beta-diol
0.003
5alpha-dihydroprogesterone
Homo sapiens
-
in 100 mM potassium phosphate, pH 7.0, at 25C
0.033
5alpha-dihydrotestosterone
Homo sapiens
-
isoform AKR1C4, at pH 7.4 and 25C
0.048 - 0.18
5beta-Androstan-3alpha-ol-17-one
0.047 - 0.3
5beta-androstane-3alpha,17beta-diol
0.002 - 0.003
5beta-pregnane-3alpha,20alpha-diol
0.083 - 0.42
9alpha,11beta-prostaglandin F2
0.000017
allopregnanolone
Homo sapiens
-
in 100 mM potassium phosphate, pH 7.0, at 25C
0.023 - 0.28
androsterone
0.032 - 0.13
chenodeoxycholic acid
0.03 - 0.2
cholic acid
0.012 - 0.085
deoxycholic acid
0.025
dihydrotestosterone
Homo sapiens
-
pH 7.5, 37C
0.032 - 0.043
lithocholic acid
0.2 - 0.58
NAD+
0.04 - 0.25
NADP+
additional information
additional information
Homo sapiens
-
Kcat-value for the 17beta-oxidation of testosterone is 0.000118 1/sec, and Kcat-value for the 17beta-reduction of androstenedione is 0.00112 1/sec
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 1.2
(R)-tetralol
4636
0.68 - 1
(S)-indan-1-ol
2600
1.2 - 1.83
(S)-tetralol
3707
3.33
3alpha-androstanediol
Homo sapiens
-
isoform AKR1C4, at pH 7.4 and 25C
5010
20.72
5alpha-androstane-3,17-dione
Homo sapiens
-
isoform AKR1C4, at pH 7.4 and 25C
1493
10.3 - 135
5alpha-androstane-3alpha,17beta-diol
2012
9.77
5alpha-dihydrotestosterone
Homo sapiens
-
isoform AKR1C4, at pH 7.4 and 25C
737
53.3 - 101.7
5beta-Androstan-3alpha-ol-17-one
2939
4.6 - 105
androsterone
477
28.3 - 46.7
chenodeoxycholic acid
1277
1 - 3.3
cholic acid
783
8.8 - 14.2
deoxycholic acid
1970
22.7
dihydrotestosterone
Homo sapiens
-
pH 7.5, 37C
2055
21.7 - 31.7
lithocholic acid
1325
166.7 - 583.3
NAD+
7
23.3 - 500
NADP+
10
additional information
additional information
Homo sapiens
-
Kcat/Km-value for the 17beta-oxidation of testosterone is 0.183 1/sec*mM, and Kcat/Km-value for the 17beta-reduction of androstenedione is 0.817 1/sec*mM
2
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00013 - 0.00015
chenodeoxycholic acid
0.0007 - 0.0009
Flufenamic acid
0.0028 - 0.0035
Hexestrol
0.0069 - 0.01
Ibuprofen
0.07 - 0.075
indomethacin
0.00007 - 0.00008
lithocholic acid
0.0016 - 0.0017
Medroxyprogesterone acetate
0.012 - 0.018
Phenolphthalein
0.00006 - 0.00008
Ursodeoxycholic acid
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26400
-
calculated from deduced amino acid sequence comprising 258 residues; SDS-PAGE
36790
-
calculated from predicted DD2 amino acid sequence of 322 residues
36850
-
calculated from predicted DD4 amino acid sequence of 321 residues
37000
-
purified DD2
39000
-
purified DD4
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 36000, SDS-PAGE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of the human 3alpha-HSD type 3 in the ternary complex with testosterone and NADP+ determined by molecular replacement method using the program EPMR, at 1.25-A resolution, binding site analysis
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in one step using metal chelate chromatography, purity of the protein is more than 98%
-
Q-Sepharose column chromatography and Sephadex G-100 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3alpha-HSD/CR gene cloned into pET15b vector with an N-terminal His tag sequence and transformation of Escherichia coli BL21(DE3) pLysS cells
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expressed in COS-1 cells, PC-3 cells, and LNCaP cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli cells
-
expressed in Escherichia coli JM109 cells
-
expressed in Huh-7 and Hep-G2 cells
-
overexpression in Escherichia coli; overexpression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
liver X receptor alpha binds specifically to the liver X receptor alpha response element and mediates transcriptional activation of the AKR1C4 gene. Liver X receptor ligand T1317 induces isoform AKR1C4 expression approximately 2.3fold
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H216F
-
the mutation decreases 3fold the Km for NADP+. The kinetic constants for bile acids with a 12alpha-hydroxy group are decreased 1.5-7fold and those for the other substrates are increased 1.3-9fold. The mutation decreases the stimulatory effects of the enzyme activity by sulfobromophthalein, clofibric acid and thyroxine, which increases the Km for the coenzyme and substrate of the mutant enzymes more highly than those of the wild type enzyme
H216L
-
inactive
H216W
-
inactive
H216Y
-
the mutation decreases 3fold the Km for NADP+. The kinetic constants for bile acids with a 12alpha-hydroxy group are decreased 1.5-7fold and those for the other substrates are increased 1.3-9fold. The mutation decreases the stimulatory effects of the enzyme activity by sulfobromophthalein, clofibric acid and thyroxine, which increases the Km for the coenzyme and substrate of the mutant enzymes more highly than those of the wild type enzyme