Information on EC 1.1.1.401 - 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD+)

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The expected taxonomic range for this enzyme is: Sulfobacillus thermosulfidooxidans

EC NUMBER
COMMENTARY hide
1.1.1.401
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RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-L-rhamnonate + NAD+ = 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-rhamnose degradation III
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-L-rhamnonate:NAD+ 4-oxidoreductase
The enzyme, characterized from the bacteria Sphingomonas sp. SKA58 and Sulfobacillus thermosulfidooxidans, is involved in the non-phosphorylative degradation pathway for L-rhamnose. It does not show any detectable activity with NADP+ or with other aldoses.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-L-rhamnonate + NAD+
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
show the reaction diagram
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?
additional information
?
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enzyme shows low activity towards 2,3-butanediol (11% of the activity with 2-dehydro-3-deoxy-L-rhamnonate), and very little activity towards L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate and L-mannonate. No substrates are: aldoses such as D-glucose, D-mannose, D-galactose, D-fructose, D-xylose, D-fucose, D-arabinose, D-lyxose, D-ribose, D-fucose, L-fucose, L-xylose, L-arabinose, L-sorbose, polyols such as sorbitol, mannitol, xylitol, amino acids such as L-threonine, L-serine, alcohols such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 1,2-propandiol, 1,3-propandiol, aldonates such as D-gluconate, D-galactonate, D-mannonate, D-arabinonate, D-lyxonate, D-xylonate, organic acids such as pyruvate, D-lactate, L-lactate, aldehydes such as D-lactaldehyde, L-lactaldehyde, acetaldehyde, DL-glyceraldehyde, glycolaldehyde
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no detectable activity with NADP+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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5 mM, 118% of initial activity
additional information
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activity is not significantly affected by the addition of 5 mM metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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5 mM, 73% residual activity
EGTA
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5 mM, 72% residual activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
2-dehydro-3-deoxy-L-rhamnonate
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pH 9.5, 50°C
0.42
NAD+
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pH 9.5, 50°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11.5
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little or no activity below pH 7.0, at pH 11.5 the enzme shows 75% of the activity at pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
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20 min, 53% residual activity