Information on EC 1.1.2.5 - D-lactate dehydrogenase (cytochrome c-553)

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The expected taxonomic range for this enzyme is: Desulfovibrio vulgaris

EC NUMBER
COMMENTARY hide
1.1.2.5
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RECOMMENDED NAME
GeneOntology No.
D-lactate dehydrogenase (cytochrome c-553)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-lactate + 2 ferricytochrome c-553 = pyruvate + 2 ferrocytochrome c-553 + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pyruvate metabolism
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SYSTEMATIC NAME
IUBMB Comments
(R)-lactate:ferricytochrome-c-553 2-oxidoreductase
The enzyme from the sulfate-reducing bacterium Desulfovibrio vulgaris can also act on (R)-2-hydroxybutanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-79-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-lactate + 1-methoxyphenazinium methyl sulfate
pyruvate + ?
show the reaction diagram
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-
-
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?
D-lactate + 2,6-dichlorophenolindophenol
pyruvate + ?
show the reaction diagram
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-
-
-
?
D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride
pyruvate + ?
show the reaction diagram
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-
-
-
?
D-lactate + ferricyanide
pyruvate + ferrocyanide
show the reaction diagram
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-
-
-
?
D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
show the reaction diagram
D-lactate + methylene blue
pyruvate + ?
show the reaction diagram
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-
-
-
?
D-lactate + nitrotetrazolium blue
pyruvate + ?
show the reaction diagram
-
-
-
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?
DL-2-hydroxybutyrate + ferricytochrome c-553
2-ketobutyrate + ferrocytochrome c-553
show the reaction diagram
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-
-
?
additional information
?
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no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-lactate + ferricytochrome c-553
pyruvate + ferrocytochrome c-553
show the reaction diagram
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starting enzyme for carbon and energy metabolism
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ferricytochrome c-553
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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slight stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
D-lactate
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2.4
DL-2-Hydroxybutyrate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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D-lactate + nitrotetrazolium blue
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 days, 20% loss of activity
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4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE