Information on EC 1.1.3.10 - pyranose oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.10
-
RECOMMENDED NAME
GeneOntology No.
pyranose oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose + O2 = 2-dehydro-D-glucose + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
pyranose:oxygen 2-oxidoreductase
A flavoprotein (FAD). Also oxidizes D-xylose, L-sorbose and D-glucono-1,5-lactone, which have the same ring conformation and configuration at C-2, C-3 and C-4.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-80-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
unidentified
-
-
Manually annotated by BRENDA team
Coriolus sp.
-
-
-
Manually annotated by BRENDA team
Corticium caeruleum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
Phanerochaete gigantea
-
-
-
Manually annotated by BRENDA team
Polyporus obtusus
-
-
-
Manually annotated by BRENDA team
Polyporus obtusus AU124PD
-
-
-
Manually annotated by BRENDA team
Saxidomus giganteus
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Trametes cinnabarinus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain MB 49
-
-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-fructose + O2
1,5-anhydro-3-keto-D-fructose + H2O2
show the reaction diagram
-
-
yield of 1,5-anhydro-3-keto-D-fructose: 33%
?
1,5-anhydro-D-glucitol + O2
1,5-anhydro-D-fructose + H2O2
show the reaction diagram
Phanerochaete gigantea
-
8% relative activity to D-glucose
yield of 1,5-anhydro-D-fructose: 100%
?
1,5-anhydro-D-glucitol + O2
?
show the reaction diagram
-
18.4% of the activity with D-glucose
-
-
?
1,5-anhydro-S-sorbitol + O2
?
show the reaction diagram
-
-
-
-
?
1,6-anhydro-D-glucose + O2
?
show the reaction diagram
-
-
-
-
?
1-beta-aurothioglucose + O2
?
show the reaction diagram
-
91% relative activity to D-glucose
-
-
?
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) + O2
? + H2O2
show the reaction diagram
-
-
-
?
2,6-dichloroindophenol + O2
? + H2O2
show the reaction diagram
-
-
-
?
2,6-dimethyl-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
2-chloro-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
-
-
-
?
2-deoxy-2-fluoro-D-glucose + O2
2-deoxy-3-dehydro-D-glucose + H2O2
show the reaction diagram
slow substrate
-
-
?
2-deoxy-D-galactose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
show the reaction diagram
2-deoxy-D-glucose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
2-keto-D-glucose + O2
2,3-diketo-D-glucose + H2O2
show the reaction diagram
2-methoxy-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
-
-
-
?
3-deoxy-D-glucose + O2
2-keto-3-deoxy-D-glucose + H2O2
show the reaction diagram
3-O-methyl-D-glucose + O2
2-keto-3-O-methyl-D-glucose + H2O2
show the reaction diagram
-
8.6% relative activity to D-glucose
-
?
5-thioglucose + O2
2-keto-5-thioglucose + H2O2
show the reaction diagram
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
show the reaction diagram
allose + O2
?
show the reaction diagram
-
38% relative activity to D-glucose
-
-
?
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
alpha-D-melibiose + O2
? + H2O2
show the reaction diagram
beta-D-galactose + O2
2-dehydro-D-galactose + H2O2
show the reaction diagram
poor substrate
-
-
?
D-allose + O2
?
show the reaction diagram
D-arabinose + O2
?
show the reaction diagram
-
1.87% of the activity with D-glucose
-
-
?
D-cellobiose + O2
?
show the reaction diagram
D-fructose + O2
2-dehydro-D-fructose + H2O2
show the reaction diagram
-
-
-
-
?
D-fructose + O2
? + H2O2
show the reaction diagram
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
show the reaction diagram
D-fucose + O2
?
show the reaction diagram
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
show the reaction diagram
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
show the reaction diagram
-
-
-
?
D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
show the reaction diagram
-
-
-
-
?
D-galactose + ferrocenium hexafluorophosphate
2-dehydro-D-galactose + ferrocene
show the reaction diagram
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
show the reaction diagram
D-galactose + O2
?
show the reaction diagram
D-glucono-1,5-lactone + O2
?
show the reaction diagram
D-gluconolactone + O2
? + H2O2
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
show the reaction diagram
1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction
-
-
?
D-glucose + 1,4-benzoquinone
?
show the reaction diagram
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
show the reaction diagram
D-glucose + 2,6-dichloroindophenol
2-dehydro-D-glucose + ?
show the reaction diagram
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dimethyl-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-methoxy-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-methyl-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + ferricenium hexafluorophosphate
?
show the reaction diagram
D-glucose + ferrocenium hexafluorophosphate
2-dehydro-D-glucose + ferrocene
show the reaction diagram
-
-
-
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ?
show the reaction diagram
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
D-glucose + O2
D-arabino-2-hexosulose + H2O2
show the reaction diagram
-
the enzyme is produced during glucose starvation
-
?
D-glucose + O2
D-arabino-hexos-2-ulose + H2O2
show the reaction diagram
D-glucose + O2
D-glucosone + H2O2
show the reaction diagram
D-glucose + tetrafluoro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-maltoheptaose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-maltopentaose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-maltose + O2
?
show the reaction diagram
-
15.1% of the activity with D-glucose
-
-
?
D-maltotriose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-mannoheptose + O2
?
show the reaction diagram
-
8.2% relative activity to D-glucose
-
-
?
D-mannose + O2
2-keto-D-mannose + H2O2
show the reaction diagram
-
0.9% relative activity to D-glucose
-
?
D-mannose + O2
?
show the reaction diagram
-
6.02% of the activity with D-glucose
-
-
?
D-melibiose + O2
?
show the reaction diagram
D-trehalose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-xylose + O2
2-dehydro-D-xylose + H2O2
show the reaction diagram
D-xylose + O2
?
show the reaction diagram
D-xylose + O2
D-threo-pentos-2-ulose + H2O2
show the reaction diagram
D-xylose + O2
D-xylosone + H2O2
show the reaction diagram
ferricenium hexafluorophosphate + O2
? + H2O2
show the reaction diagram
-
-
-
?
gentibiose + O2
2-keto-D-gentibiose + H2O2
show the reaction diagram
L-arabinose + O2
?
show the reaction diagram
L-idose + O2
?
show the reaction diagram
-
-
-
-
?
L-sorbose + O2
2-dehydro-L-sorbose + H2O2
show the reaction diagram
L-sorbose + O2
5-dehydro-D-fructose + H2O2
show the reaction diagram
L-sorbose + O2
?
show the reaction diagram
L-sorbose D-allose + O2
?
show the reaction diagram
-
-
-
?
maltose + O2
? + H2O2
show the reaction diagram
melibiose + O2
?
show the reaction diagram
-
-
-
?
methyl alpha-D-glucoside + O2
2-dehydro-alpha-D-methylglucoside + H2O2
show the reaction diagram
methyl beta-D-glucoside + O2
2-dehydro-beta-D-methylglucoside + H2O2
show the reaction diagram
methyl-beta-D-glucoside + O2
?
show the reaction diagram
-
-
-
?
tetrafluoro-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
trehalose + O2
?
show the reaction diagram
-
14.3% of the activity with D-glucose
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
Coriolus sp.
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
show the reaction diagram
-
mutants T169S, T169N, and T169G
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
D-glucose + O2
D-arabino-2-hexosulose + H2O2
show the reaction diagram
-
the enzyme is produced during glucose starvation
-
?
D-xylose + O2
D-xylosone + H2O2
show the reaction diagram
Coriolus sp.
-
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
show the reaction diagram
Coriolus sp.
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
slightly stimulates at 1 mM
Co2+
-
slightly stimulates at 1 mM
Fe2+
-
stimulation
Mg2+
-
slightly stimulates at 1 mM
Zn2+
-
slightly stimulates at 1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-keto-D-glucose
-
5% reduction at 100 mM, 10% reduction at 200 mM, no deactivating effect determined
2-mercaptoethanol
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
8-hydroxyquinoline
acetate
alpha,alpha'-dipyridyl
ammonium sulfate
-
slightly inhibits
ascorbic acid
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
Co2+
-
13.3% inhibition
D-glucose
Polyporus obtusus
-
inhibits the oxidation of gluconolactone
D-xylose
Polyporus obtusus
-
inhibits the oxidation of gluconolactone
Dithionite
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
dithiothreitol
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
ethanol
Fe2+
-
completely inhibits at 1 mM
glutathione
Coriolus sp.
-
-
hydrogen peroxide
-
inactivation determined
L-sorbose
Polyporus obtusus
-
inhibits the oxidation of gluconolactone
Lead acetate
-
50% inhibition at 10 mM
Mn2+
-
50% inhibition at 10 mM
N-bromosuccinimide
NaCl
-
slightly inhibits at concentrations higher than 8%
Ni2+
-
28.6% inhibition
p-chloromercuribenzoate
Propionate
-
-
reduced glutathione
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
Sodium azide
-
slightly inhibitory at 1 mM, more than 85% activity remains
valerate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
catalase
-
the presence of catalase increases the rate of reaction
-
NaCl
-
up to 8%
additional information
-
an increase in the pressure with compressed air results in higher rates of bioconversion of D-glucose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0052 - 2
1,4-benzoquinone
10.7
1,5-Anhydro-D-glucitol
-
pH 7.0, 37C
0.07 - 0.09
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical
-
0.051 - 0.39
2,6-dichloroindophenol
0.4 - 2.1
2,6-dimethyl-1,4-benzoquinone
0.31 - 0.48
2-chloro-1,4-benzoquinone
10.32
2-deoxy-D-galactose
100 mM ethanolamine buffer, pH 10.5
11.3 - 99.3
2-deoxy-D-glucose
0.21 - 0.42
2-methoxy-1,4-benzoquinone
0.11 - 0.51
2-methyl-1,4-benzoquinone
3.9
5-thioglucose
-
-
1 - 1.53
alpha-D-glucose
0.57 - 0.97
beta-D-glucose
22.1 - 68
D-Allose
174
D-arabinose
-
pH 7.0, 37C
0.71
D-cellobiose
100 mM ethanolamine buffer, pH 10.5
103 - 2558
D-fructose
6.25
D-fucose
100 mM ethanolamine buffer, pH 10.5
0.093 - 1260
D-galactose
38
D-gluconolactone
-
-
0.22 - 240
D-glucose
6.02
D-maltoheptaose
100 mM ethanolamine buffer, pH 10.5
6.14
D-maltopentaose
100 mM ethanolamine buffer, pH 10.5
329
D-maltose
-
pH 7.0, 37C
6.61
D-maltotriose
100 mM ethanolamine buffer, pH 10.5
110
D-mannoheptose
-
-
295
D-mannose
-
pH 7.0, 37C
2 - 1500
D-melibiose
120
D-melibose
-
-
-
7.51
D-trehalose
100 mM ethanolamine buffer, pH 10.5
2.4 - 78.4
D-xylose
0.092 - 1.12
ferricenium hexafluorophosphate
0.054 - 0.408
ferricenium ion
3.43
ferricyanide
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30C, pH 6.5
0.015 - 0.4
ferrocenium hexafluorophosphate
0.187
ferrocenium ion
using D-glucose as cosubstrate, at 30C, pH 6.5
62
gentibiose
-
-
0.79 - 0.85
glucose
1.55 - 97
L-arabinose
2.9 - 176
L-sorbose
257
maltose
-
oxygen concentration 1.07 mM
72.8
melibiose
at 30C, pH 6.5
0.35 - 0.4
methyl-1,4-benzoquinone
50 - 289
methyl-beta-D-glucoside
0.03 - 4.8
O2
0.0832 - 0.65
oxygen
0.09
tetrabromo-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.088
tetrachloro-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.22 - 9.42
tetrafluoro-1,4-benzoquinone
192
trehalose
-
pH 7.0, 37C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 477
1,4-benzoquinone
76.7
1,5-Anhydro-D-glucitol
Tricholoma matsutake
-
pH 7.0, 37C
1.33
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical
Phanerochaete chrysosporium
Q6QWR1
substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30C, pH 6.5
-
13.1 - 108
2,6-dichloroindophenol
0.4 - 8.69
2,6-dimethyl-1,4-benzoquinone
0.15 - 16.3
2-chloro-1,4-benzoquinone
40.1 - 47.2
2-methoxy-1,4-benzoquinone
0.12 - 10.1
2-methyl-1,4-benzoquinone
7.79
D-arabinose
Tricholoma matsutake
-
pH 7.0, 37C
1.88 - 5.55
D-fructose
0.02 - 171
D-galactose
0.006 - 615
D-glucose
62.9
D-maltose
Tricholoma matsutake
-
pH 7.0, 37C
25.1
D-mannose
Tricholoma matsutake
-
pH 7.0, 37C
0.19 - 7.6
D-melibiose
0.27 - 55.8
D-xylose
1.7 - 549
ferricenium hexafluorophosphate
1.44 - 334
ferricenium ion
0.3
ferricyanide
Phanerochaete chrysosporium
Q6QWR1
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30C, pH 6.5
2.9 - 470
ferrocenium hexafluorophosphate
39.9
ferrocenium ion
Lyophyllum shimeji
Q75ZP8
using D-glucose as cosubstrate, at 30C, pH 6.5
49
glucose
Trametes ochracea
-
soluble enzyme, O2 used as electron acceptor, determined in cuvette assay, pH 5.0
1.59 - 74.6
L-sorbose
0.839
melibiose
Lyophyllum shimeji
Q75ZP8
at 30C, pH 6.5
5.9 - 109
O2
6.61 - 129
tetrafluoro-1,4-benzoquinone
59.6
trehalose
Tricholoma matsutake
-
pH 7.0, 37C