Information on EC 1.1.3.18 - secondary-alcohol oxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.18
-
RECOMMENDED NAME
GeneOntology No.
secondary-alcohol oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a secondary alcohol + O2 = a ketone + H2O2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
secondary-alcohol:oxygen oxidoreductase
Acts on secondary alcohols with five or more carbons, and polyvinyl alcohols with molecular mass over 300 Da. The Pseudomonas enzyme contains one atom of non-heme iron per molecule.
CAS REGISTRY NUMBER
COMMENTARY hide
71245-08-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mixed culture of 3 different soil bacteria
-
-
Manually annotated by BRENDA team
var. povalolyticus PH
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida
strain VM15A
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida VM15A
strain VM15A
-
-
Manually annotated by BRENDA team
strain WSH02-21
-
-
Manually annotated by BRENDA team
strain WSH02-21
-
-
Manually annotated by BRENDA team
4 strains isolated from oil polluted soil
-
-
Manually annotated by BRENDA team
strain VM15C
-
-
Manually annotated by BRENDA team
CCM 7450, growth and PVA degradation supported by co-isolated strain Rhodococcus erythropolis OT3
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,10-decanediol + O2
1,10-decanedione + H2O2
show the reaction diagram
1,2,6-hexanetriol + O2
1,2,6-hexanetrione + H2O2
show the reaction diagram
1,2-butanediol + O2
1,2-butanedione + H2O2
show the reaction diagram
-
-
-
-
?
1,2-hexanediol + O2
1,2-hexanedione + H2O2
show the reaction diagram
-
-
-
-
?
1,2-propanediol + O2
1,2-propanedione + H2O2
show the reaction diagram
1,3-butanediol + O2
1,3-butanedione + H2O2
show the reaction diagram
-
-
-
-
?
1,3-cyclohexanediol + O2
1,3-cyclohexanedione + H2O2
show the reaction diagram
1,4-benzenedimethanol + O2
benzene-1,4-dicarboxyaldehyde + H2O2
show the reaction diagram
1,4-butanediol + O2
1,4-butanedione + H2O2
show the reaction diagram
1,5-pentanediol + O2
1,5-pentanedione + H2O2
show the reaction diagram
1,6-hexanediol + O2
1,6-hexanedione + H2O2
show the reaction diagram
1,8-octanediol + O2
1,8-octanedione + H2O2
show the reaction diagram
1-butanol + O2
n-butanal + H2O2
show the reaction diagram
1-decanol + O2
n-decanal + H2O2
show the reaction diagram
-
-
-
-
?
1-heptanol + O2
n-heptanal + H2O2
show the reaction diagram
1-hexanol + O2
n-hexanal + H2O2
show the reaction diagram
1-nonanol + O2
n-nonanal + H2O2
show the reaction diagram
1-pentanol + O2
n-pentanal + H2O2
show the reaction diagram
1-propanol + O2
n-propanal + H2O2
show the reaction diagram
2,4-pentanediol + O2
2,4-pentanedione + H2O2
show the reaction diagram
-
-
-
-
?
2,5-hexanediol + O2
2,5-hexanedione + H2O2
show the reaction diagram
2-butanol + O2
2-butanone + H2O2
show the reaction diagram
2-butanol + O2
butan-2-one + H2O2
show the reaction diagram
-
-
-
-
?
2-decanol + O2
2-decanone + H2O2
show the reaction diagram
2-heptanol + O2
2-heptanone + H2O2
show the reaction diagram
2-hexanol + O2
2-hexanone + H2O2
show the reaction diagram
2-hexanol + O2
hexan-2-one + H2O2
show the reaction diagram
-
low activity
-
-
?
2-nonanol + O2
2-nonanone + H2O2
show the reaction diagram
2-octanol + O2
2-octanone + H2O2
show the reaction diagram
2-octanol + O2
octan-2-one + H2O2
show the reaction diagram
2-pentanol + O2
2-pentanone + H2O2
show the reaction diagram
2-propanol + O2
2-propanone + H2O2
show the reaction diagram
2-propanol + O2
propan-2-one + H2O2
show the reaction diagram
3-heptanol + O2
3-heptanone + H2O2
show the reaction diagram
3-hexanol + O2
3-hexanone + H2O2
show the reaction diagram
3-hydroxy-5-nonanone + O2
nonan-3,5-dione + H2O2
show the reaction diagram
-
-
-
-
?
3-nonanol + O2
3-nonanone + H2O2
show the reaction diagram
3-octanol + O2
3-octanone + H2O2
show the reaction diagram
3-octanol + O2
octan-3-one + H2O2
show the reaction diagram
-
-
-
-
?
3-pentanol + O2
3-pentanone + H2O2
show the reaction diagram
4,6-nonanediol + O2
4,6-nonanedione + 6-hydroxy-4-nonanone + H2O2
show the reaction diagram
-
2 reaction products detected by gas chromatography
-
-
?
4-decanol + O2
4-decanone + H2O2
show the reaction diagram
4-heptanol + O2
4-heptanone + H2O2
show the reaction diagram
4-hydroxy-2-nonanone + O2
nonan-2,4-dione + H2O2
show the reaction diagram
-
-
-
-
?
4-nonanol + O2
4-nonanone + H2O2
show the reaction diagram
4-octanol + O2
4-octanone + H2O2
show the reaction diagram
5-decanol + O2
5-decanone + H2O2
show the reaction diagram
5-hydroxy-3-heptanone + O2
heptan-3,5-dione + H2O2
show the reaction diagram
-
-
-
-
?
5-nonanol + O2
5-nonanone + H2O2
show the reaction diagram
6-hydroxy-4-nonanone + O2
4,6-nonanedione + H2O2
show the reaction diagram
-
-
-
-
?
6-hydroxy-4-nonanone + O2
nonan-4,6-dione + H2O2
show the reaction diagram
-
-
-
-
?
6-undecanol + O2
6-undecanone + H2O2
show the reaction diagram
7-hydroxy-5-dodecanone + O2
dodecan-5,7-dione + H2O2
show the reaction diagram
-
-
-
-
?
8-hydroxy-6-tridecanone + O2
tridecan-6,8-dione + H2O2
show the reaction diagram
-
-
-
-
?
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
catechol + O2
o-benzoquinone + H2O2
show the reaction diagram
-
-
-
-
?
cyclohexanol + O2
cyclohexanone + H2O2
show the reaction diagram
cyclooctanol + O2
cyclooctanone + H2O2
show the reaction diagram
-
-
-
-
?
isoamyl alcohol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
l-menthol + O2
menthone + H2O2
show the reaction diagram
-
-
-
-
?
octanol + O2
n-octanal + H2O2
show the reaction diagram
-
-
-
-
?
phenethyl alcohol + O2
phenylacetaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
polyvinyl alcohol + 2,6-dichlorophenolindophenol
polyvinylketone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
polyvinyl alcohol + cytochrome c
polyvinylketone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
polyvinyl alcohol + ferricyanide
polyvinylketone + ferrocyanide
show the reaction diagram
polyvinyl alcohol + NAD+
polyvinylketone + H2O2
show the reaction diagram
-
-
-
-
?
polyvinyl alcohol + NADP+
polyvinylketone + H2O2
show the reaction diagram
-
-
-
-
?
polyvinyl alcohol + nitro blue tetrazolium
polyvinylketone + reduced nitro blue tetrazolium
show the reaction diagram
polyvinyl alcohol + O2
polyvinylketone + H2O2
show the reaction diagram
polyvinyl alcohol + phenazine ethosulfate
polyvinylketone + reduced phenazine ethosulfate
show the reaction diagram
-
-
-
-
?
sec-phenethyl alcohol + O2
1-phenylethanone + H2O2
show the reaction diagram
tert-butanol + O2
tert-butanone + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-butanol + O2
butan-2-one + H2O2
show the reaction diagram
-
-
-
-
?
2-propanol + O2
propan-2-one + H2O2
show the reaction diagram
-
-
-
-
?
polyvinyl alcohol + O2
polyvinylketone + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
relative activity 106%
Co2+
-
catalyzes the decompositon of H2O2
Mg2+
-
relative activity 103%
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
BaCl2
Cu2+
-
weak inhibition
hydroxylamine
iodoacetamide
-
-
MnCl2
NiCl2
o-phenanthroline
Pb2+
-
weak inhibition
salicylaldoxine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-Dinitrophenol
-
activity 101.6%
8-hydroxyquinoline
-
1 mM, relative activity 110%
alpha,alpha'-dipyridyl
-
activity 102%
iodoacetamide
-
1 mM, relative activity 101%
KCN
-
1 mM, relative activity 101%
Monoiodoacetic acid
-
activity 102.5%
Na3AsO3
-
activity 101.4%
-
NaN3
-
1 mM, relative activity 102%
additional information
-
yet unknown growth factor supplied by co-isolated Rhodococcus erythropolis strain
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
4-heptanol
-
-
1
polyvinyl alcohol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0018
-
in association with the co-isolated Rhodococcus erythropolis strain, isolate able to degrade 500 mg of polyvinyl alcohol per litre in 2 weeks, no oxidase activity on 2-butanol, 2,4-pentanediol, 2-hexanol, 2-heptanol detected
3.8
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
-
8 - 8.4
-
-
9.6
-
purified enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
-
30 - 50
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
high SAO activity is observed in the lag phase
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
activity associated with cells of co-isolated degrader strain Rhodococcus erythropolis
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
gel filtration
38500
-
sedimentation equilibrium analysis, gel filtration Sephadex G-100
40000
-
gel filtration
49500
-
sedimentation equilibrium
50500
-
SDS-PAGE
75000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9
-
stable between
348177
5 - 9
-
stable between
348181
5 - 11
-
stable in this range at 5C, above pH 11 activity decreases rapidly
348174
6 - 10
-
stable between
287626
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
activity remains 100% below 50C, completely lost at 70C
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
2-butanol induces the enzyme
-