Information on EC 1.1.3.19 - 4-hydroxymandelate oxidase (decarboxylating)

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.1.3.19
-
RECOMMENDED NAME
GeneOntology No.
4-hydroxymandelate oxidase (decarboxylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-4-hydroxymandelate + O2 = 4-hydroxybenzaldehyde + CO2 + H2O2
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
-
Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
(S)-4-hydroxymandelate:oxygen 1-oxidoreductase (decarboxylating)
A flavoprotein (FAD), requires Mn2+. The enzyme from the bacterium Pseudomonas putida is involved in the degradation of mandelate.
CAS REGISTRY NUMBER
COMMENTARY hide
60976-30-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2
4-hydroxybenzaldehyde + CO2 + H2O2
show the reaction diagram
DL-4-hydroxymandelate + ?
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2
4-hydroxybenzaldehyde + CO2 + H2O2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-Dinitrophenylhydrazine
-
-
2-mercaptoethanol
-
-
8-hydroxyquinoline
-
-
cysteine
-
-
diethyldithiocarbamate
-
-
dithiothreitol
-
-
DL-3,4-dihydroxymandelic acid
-
-
guanidine hydrochloride
-
-
hydroxylamine
-
-
potassium cyanide
-
-
reduced glutathione
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.44
DL-4-hydroxymandelate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
155000
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after solubilization the enzyme gradually loses activity when kept at 5°C, full reactivation by freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 months, solubilized enzyme
-
-20°C, particulate fraction suspended in 0.025 M sodium phosphate buffer, pH 7.0, more than 6 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE