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1,4-androstadiene-3,17-dione + H2O
?
-
11% of the activity with cholesterol, enzyme CO1
-
-
?
1-propanol + O2
propanal + H2O2
-
-
-
?
17beta-hydroxymethyl-5-androsten-3beta-ol + O2
17beta-hydroxymethyl-5-androsten-3-one + H2O2
Nocardia erythropolis
-
-
-
?
20,22-dihydroxycholesterol + O2
20,22-dihydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
20,25-diazacholesterol + O2
20,25-diazacholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
20-hydroxycholesterol + O2
20-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
22-hydroxycholesterol + O2
22-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
23,24-dinor-5-cholen-3beta,22-diol + O2
23,24-dinor-5-cholen-22-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
23,24-dinor-5-cholene-3beta,22-diol + O2
23,24-dinor-5-cholen-22-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
-
r
24-hydroxycholesterol + O2
24-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
24-oxocholesterol + O2
24-oxocholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
25-hydroxy-27-norcholesterol + O2
?
Nocardia erythropolis
-
-
-
-
?
25-hydroxycholesterol + O2
25-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
26-hydroxycholesterol + O2
26-hydroxycholest-5-en-3-one + H2O2
3-betacholestanol + O2
? + H2O2
3beta-chlorocholest-5-ene + O2
? + H2O2
3beta-hydroxyandrost-5-en-17-one + O2
androst-5-en-3,17-dione + H2O2
3beta-hydroxypregn-5-en-20-one + O2
pregn-5-en-3,20-dione + H2O2
4,4-dimethylcholesterol + O2
4,4-dimethylcholest-5-en-3-one
Nocardia erythropolis
-
-
-
?
4alpha-methylcholesterol + O2
4alpha-methylcholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
4beta-methylcholesterol + O2
4beta-methylcholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5,7-cholestadien-3beta-ol + O2
5,7-cholestadien-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5-androstene-3beta,17beta-diol + O2
5-androsten-17beta-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5-cholene-3beta,24-diol + O2
5-cholen-24-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5-pregnen-3,20-dione + O2
pregnenolone + H2O2
Nocardia erythropolis
-
-
-
-
r
5-pregnen-3beta,20beta-diol + O2
5-pregnen-20beta-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5alpha-androstan-3-ol-17-one + O2
5alpha-androstan-3,17-dione + H2O2
-
15% of the activity with cholesterol
-
-
?
5alpha-cholest-8(14)-en-3beta-ol + O2
5alpha-cholest-8(14)-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
5alpha-cholestane-3beta-ol-7-one + O2
5alpha-cholestane-3,7,dione
7-dehydrocholesterol + O2
cholest-4,7-dien-3-one + H2O2
7-keto-cholesterol + O2
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
androst-4-ene-3,17-dione + O2
? + H2O2
-
-
-
?
beta-cholestanol + O2
? + H2O2
beta-sitosterol + O2
? + H2O2
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
beta-stigmasterol + O2
?
-
36% of the activity with cholesterol
-
-
?
beta-stigmasterol + O2
? + H2O2
-
68% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
cholest-4-en-3-one + O2
? + H2O2
-
-
-
?
cholest-5-en-3beta-ol 7-one + O2
cholest-5-en-3,17-dione + H2O2
-
low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
cholestenol + O2
5alpha-cholest-8-en-3-one + H2O2
-
43% activity compared to cholesterol
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
cholesterol + O2
cholest-4-en-3-one + H2O2
cholesterol + O2
cholest-5-en-3-one + H2O2
cholesterol-5alpha,6beta-epoxide + O2
?
cyclohexanol + O2
cyclohexanal + H2O2
-
-
-
?
dehydro-epi-androsterone + O2
?
dehydroandrosterone + O2
? + H2O2
-
-
-
?
dehydroepiandrosterone + O2
?
-
27% of the activity with cholesterol
-
-
?
dehydroepiandrosterone + O2
? + H2O2
-
30% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
dehydroisoandrosterone + O2
androst-5-en-3,17-dione + H2O
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
diosgenin + O2
? + H2O2
-
-
-
?
diosgenin + O2
diosgenone + H2O2
epiandrosterone + O2
? + H2O2
-
15% activity compared to cholesterol
-
-
?
ergosta-5,7,22-trien-3beta-ol + O2
ergostan-5,7,22-trien-3-one + H2O2
-
18% of the activity with cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
estrone + O2
estra-1,5(10)-dien-3,17-dione + H2O2
-
low activity with wild-type enzyme, no activity with mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
ethanol + O2
ethanal + H2O2
-
-
-
?
fucosterol + O2
stigmasta-5,24(28)-dien-3-one + H2O2
hecogenin + O2
5alpha-(25R)-spirostan-3,12-dione + H2O2
lanosterol + O2
5alpha-lanosta-8,24-dien-3-one + H2O2
lanosterol + O2
lanosta-8,24-dien-3-one + H2O2
-
slow rate
-
?
methanol + O2
methanal + H2O2
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
pregnenolone + O2
? + H2O2
pregnenolone + O2
pregn-5-ene-3,20-dione + H2O2
low activity
-
-
?
sitost-5-en-3beta-ol + O2
sitost-5-en-3-one + H2O2
-
88% of the activity with cholesterol
-
-
?
stigmast-5-en-3beta-ol + O2
stigmast-5-en-3-one + H2O2
-
68% of the activity with cholesterol
-
-
?
stigmastanol + O2
stigmastanone + H2O2
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
taurocholate + O2
? + H2O2
trans-androsterone + O2
?
trans-dehydroandrosterone + O2
?
additional information
?
-
26-hydroxycholesterol + O2
26-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
26-hydroxycholesterol + O2
26-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
-
?
3-betacholestanol + O2
? + H2O2
-
-
-
?
3-betacholestanol + O2
? + H2O2
-
-
-
?
3beta-chlorocholest-5-ene + O2
? + H2O2
-
-
-
?
3beta-chlorocholest-5-ene + O2
? + H2O2
low activity
-
-
?
3beta-hydroxyandrost-5-en-17-one + O2
androst-5-en-3,17-dione + H2O2
-
30% of the activity with cholesterol
-
-
?
3beta-hydroxyandrost-5-en-17-one + O2
androst-5-en-3,17-dione + H2O2
-
30% of the activity with cholesterol
-
-
?
3beta-hydroxypregn-5-en-20-one + O2
pregn-5-en-3,20-dione + H2O2
-
42% of the activity with cholesterol
-
-
?
3beta-hydroxypregn-5-en-20-one + O2
pregn-5-en-3,20-dione + H2O2
-
42% of the activity with cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
13.2% of activity with cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
Nocardia erythropolis
-
87% of activity
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
equally active as cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
equally active as cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
?
5alpha-cholestane + O2
?
-
72% of the activity with cholesterol, enzyme CO1
-
-
?
5alpha-cholestane + O2
?
-
90% of the activity with cholesterol, enzyme CO2
-
-
?
5alpha-cholestane-3beta-ol-7-one + O2
5alpha-cholestane-3,7,dione
-
74% of the activity with cholesterol, enzyme CO1
-
-
?
5alpha-cholestane-3beta-ol-7-one + O2
5alpha-cholestane-3,7,dione
-
84% of the activity with cholesterol, enzyme CO2
-
-
?
7-dehydrocholesterol + O2
cholest-4,7-dien-3-one + H2O2
-
14% of the activity with cholesterol
-
-
?
7-dehydrocholesterol + O2
cholest-4,7-dien-3-one + H2O2
-
26% of the activity with cholesterol
-
-
?
7-keto-cholesterol + O2
?
-
-
-
?
7-keto-cholesterol + O2
?
-
-
-
-
?
7-keto-cholesterol + O2
?
-
-
-
-
?
7-keto-cholesterol + O2
?
-
-
-
-
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
?
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
-
-
-
?
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
Nocardia erythropolis
-
-
-
?
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
-
-
-
?
beta-cholestanol + O2
?
13% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
69% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
95% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
95% of the activity with cholesterol
-
-
?
beta-cholestanol + O2
?
-
78% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
78% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
75% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
101% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
Streptoverticillium cholesterolieum
-
96% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
79% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
79% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
? + H2O2
-
96% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
? + H2O2
-
96% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
?
-
-
-
?
beta-sitosterol + O2
?
-
about 55% of the activity with cholesterol, cholesterol oxidase II
-
-
?
beta-sitosterol + O2
?
-
80% of the activity with cholesterol
-
-
?
beta-sitosterol + O2
?
-
-
-
-
?
beta-sitosterol + O2
?
-
-
-
-
?
beta-sitosterol + O2
?
-
-
-
-
?
beta-sitosterol + O2
? + H2O2
-
-
-
?
beta-sitosterol + O2
? + H2O2
-
-
-
-
?
beta-sitosterol + O2
? + H2O2
-
88% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
? + H2O2
-
88% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
57% of the activity with cholesterol, enzyme CO1
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
79% of the activity with cholesterol, enzyme CO2
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
57% of the activity with cholesterol, enzyme CO1
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
79% of the activity with cholesterol, enzyme CO2
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
19.7% of activity with cholesterol
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
about 30% of the activity with cholesterol, cholesterol oxidase I
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
20% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
84% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
84% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
80% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
50% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
19% of the activity with cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% of the activity with cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
85% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
85% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
72% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Streptoverticillium cholesterolieum
-
39% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
10% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
59% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
36% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Corynebacterium cholesterolicum
-
12% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
47% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
40% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
40% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
33% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
83% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
83% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
33% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
37% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
50% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Streptoverticillium cholesterolieum
-
17% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% activity compared to cholesterol
-
-
?
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
-
-
-
?
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
Nocardia erythropolis
-
67% of activity with cholesterol
-
?
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
-
-
-
?
cholestanol + O2
?
-
-
-
-
?
cholestanol + O2
?
-
-
-
?
cholestanol + O2
?
-
-
-
-
?
cholestanol + O2
?
-
-
-
-
?
cholestanol + O2
?
-
-
-
-
?
cholestenone + O2
?
-
-
-
?
cholestenone + O2
?
-
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Corynebacterium cholesterolicum
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Corynebacterium cholesterolicum
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Corynebacterium cholesterolicum
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
the assumed intermediate 5-cholesten-3-one is rapidly isomerized by the enzyme to cholest-4-en-3-one
r
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
relatively unreactive 17keto-DELTA5-3beta-hydroxysteroids are converted to satisfactory substrates by formation of isopentyloximes or benzyloximes
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
size and shape of steroid 17beta-side-chain and oxygenation of nucleus
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
enzyme specifically oxidizes 3beta-hydroxy groups in steroids
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
ir
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
enzyme specifically oxidizes 3beta-hydroxy groups in steroids
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Pseudomonas spp.
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
highest activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
best substrate
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
highest activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
best substrate
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
cholesterol oxidase is required for the biosynthesis of the antifungal polyene pimaricin
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
cholesterol oxidase is required for the biosynthesis of the antifungal polyene pimaricin, apparently acting as an antifungal sensor
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
via cholest-5-en-3-one
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
via cholest-5-en-3-one
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Streptoverticillium cholesterolieum
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Streptoverticillum cholesterolicum
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
r
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
subsequent isomerization to cholest-4-en-3-one
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
assay with phenazine sulfate and dichlorophenylindophenol as electron acceptors
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
a combination of molecular docking and molecular dynamics simulations is used to predict the binding interactions of CHL in the active site of ChOx. The hydrophobic triad residues (Y107A, F444 and Y446A) are observed to change conformation upon CHL binding in the wild-type form of the enzyme, leading to an optimal binding mode for catalytic efficiency
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
the enzyme catalyzes the oxidation of the substrate via a hydride transfer mechanism and concomitant reduction of the FAD cofactor. The Glu361 residue acts as a catalytic base facilitating the hydride transfer from the substrate to the cofactor
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
cholesterol-5alpha,6beta-epoxide + O2
?
-
10% of the activity with cholesterol, enzyme CO2
-
-
?
cholesterol-5alpha,6beta-epoxide + O2
?
-
9% of the activity with cholesterol, enzyme CO1
-
-
?
coprostane + O2
?
-
63% of the activity with cholesterol, enzyme CO1
-
-
?
coprostane + O2
?
-
67% of the activity with cholesterol, enzyme CO2
-
-
?
dehydro-epi-androsterone + O2
?
-
about 35% of the activity with cholesterol, cholesterol oxidase I
-
-
?
dehydro-epi-androsterone + O2
?
-
about 5% of the activity with cholesterol, cholesterol oxidase II
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
41.4% of the activity with cholesterol
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
41% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
16% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
16% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
27% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Corynebacterium cholesterolicum
-
5% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Nocardia erythropolis
-
-
-
r
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
28% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
24% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
37% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Streptoverticillium cholesterolieum
-
48% activity compared to cholesterol
-
-
?
dehydroisoandrosterone + O2
androst-5-en-3,17-dione + H2O
-
-
-
?
dehydroisoandrosterone + O2
androst-5-en-3,17-dione + H2O
-
low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
-
-
-
?
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
Nocardia erythropolis
-
98% of activity with cholesteol
-
?
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
-
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
58% of the activity with cholesterol, enzyme CO1
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
70% of the activity with cholesterol, enzyme CO2
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
22% of the activity with cholesterol
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
79% of the activity with cholesterol
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
-
?
diosgenin + O2
diosgenone + H2O2
i.e. (25R)-spirost-5-en-3beta-ol
-
-
?
diosgenin + O2
diosgenone + H2O2
i.e. (25R)-spirost-5-en-3beta-ol
-
-
?
epiandrosterone + O2
?
10% activity compared to cholesterol
-
-
?
epiandrosterone + O2
?
15% activity compared to cholesterol
-
-
?
epiandrosterone + O2
?
-
15% of the activity with cholesterol
-
-
?
epiandrosterone + O2
?
-
1% activity compared to cholesterol
-
-
?
epiandrosterone + O2
?
-
-
-
-
?
ergosterol + O2
?
-
-
-
?
ergosterol + O2
?
-
12% of the activity with cholesterol
-
-
?
ergosterol + O2
?
-
-
-
-
?
ergosterol + O2
?
-
22% of the activity with cholesterol
-
-
?
ergosterol + O2
?
-
-
-
-
?
ergosterol + O2
?
-
-
-
-
?
ergosterol + O2
? + H2O2
-
22% activity compared to cholesterol
-
-
?
ergosterol + O2
? + H2O2
-
12% activity compared to cholesterol
-
-
?
ergosterol + O2
? + H2O2
-
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
20% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
20% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Corynebacterium cholesterolicum
-
4% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Nocardia erythropolis
-
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
low activity
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
17% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
51% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
51% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
14% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Streptoverticillium cholesterolieum
-
5% activity compared to cholesterol
-
-
?
fucosterol + O2
stigmasta-5,24(28)-dien-3-one + H2O2
Nocardia erythropolis
-
-
-
-
?
fucosterol + O2
stigmasta-5,24(28)-dien-3-one + H2O2
-
-
-
?
hecogenin + O2
5alpha-(25R)-spirostan-3,12-dione + H2O2
-
66% of the activity with cholesterol, enzyme CO1
-
-
?
hecogenin + O2
5alpha-(25R)-spirostan-3,12-dione + H2O2
-
80% of the activity with cholesterol, enzyme CO2
-
-
?
lanosterol + O2
5alpha-lanosta-8,24-dien-3-one + H2O2
-
4% of the activity with cholesterol, enzyme CO1
-
-
?
lanosterol + O2
5alpha-lanosta-8,24-dien-3-one + H2O2
-
5% of the activity with cholesterol, enzyme CO2
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
22.4% of activity with chelesterol
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
22% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
32% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
32% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
95% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Corynebacterium cholesterolicum
-
38% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Nocardia erythropolis
-
-
-
r
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
6% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
20% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
99% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
42% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Streptoverticillium cholesterolieum
-
37% activity compared to cholesterol
-
-
?
pregnenolone + O2
?
-
-
-
-
?
pregnenolone + O2
?
-
-
-
?
pregnenolone + O2
?
-
about 60% of the activity with cholesterol, cholesterol oxidase I
-
-
?
pregnenolone + O2
?
-
about 60% of the activity with cholesterol, cholesterol oxidase II
-
-
?
pregnenolone + O2
?
-
95% of the activity with cholesterol
-
-
?
pregnenolone + O2
?
-
-
-
-
?
pregnenolone + O2
?
-
-
-
-
?
pregnenolone + O2
?
-
-
-
-
?
pregnenolone + O2
?
-
-
-
-
?
pregnenolone + O2
? + H2O2
-
-
-
?
pregnenolone + O2
? + H2O2
-
46% activity compared to cholesterol
-
-
?
pregnenolone + O2
? + H2O2
-
46% activity compared to cholesterol
-
-
?
sitosterol + O2
?
-
-
-
?
sitosterol + O2
?
-
-
-
?
stigmastanol + O2
stigmastanone + H2O2
-
15% of the activity with cholesterol
-
-
?
stigmastanol + O2
stigmastanone + H2O2
-
7% of the activity with cholesterol
-
-
?
stigmastanol + O2
stigmastanone + H2O2
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
102% of the activity with cholesterol, enzyme CO2
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
86% of the activity with cholesterol, enzyme CO1
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
102% of the activity with cholesterol, enzyme CO2
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
86% of the activity with cholesterol, enzyme CO1
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
10% of activity with cholesterol
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
about 10% of the activity with cholesterol, cholesterol oxidase I
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
about 10% of the activity with cholesterol, cholesterol oxidase II
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
15% of the activity with cholesterol
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% of the activity with cholesterol
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Nocardia erythropolis
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
taurocholate + O2
? + H2O2
-
-
-
?
taurocholate + O2
? + H2O2
-
-
-
?
trans-androsterone + O2
?
-
-
-
-
?
trans-androsterone + O2
?
-
-
-
-
?
trans-dehydroandrosterone + O2
?
-
-
-
-
?
trans-dehydroandrosterone + O2
?
-
-
-
-
?
additional information
?
-
-
optimization of assay conditions, concerning buffer type and molarity, temperature, pH, deergent addition, ionogenic state etc.
-
-
?
additional information
?
-
no activity with ergosterol
-
-
?
additional information
?
-
-
no activity with epicholesterol
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
the enzyme also acts as a DELTA5-ketosteroid isomerase, EC 5.3.3.1, converting a 3-oxo-DELTA5-steroid to a 3-oxo-DELTA4-steroid
-
-
?
additional information
?
-
-
the enzyme also acts as a DELTA5-ketosteroid isomerase, EC 5.3.3.1, converting a 3-oxo-DELTA5-steroid to a 3-oxo-DELTA4-steroid
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
the enzyme also acts as a DELTA5-ketosteroid isomerase, EC 5.3.3.1, converting a 3-oxo-DELTA5-steroid to a 3-oxo-DELTA4-steroid
-
-
?
additional information
?
-
-
less than 3% of the activity with cholesterol: ergosterol and epiandrosterone. No activity: dehydroepiandrosterone, pregnenolone
-
-
?
additional information
?
-
-
no activity with: ergosterol, dehydroepiandrosterone, epiandrosterone, pregnenolone
-
-
?
additional information
?
-
-
intact ChoD is important for the growth of Mycobacterium tuberculosis in peritoneal macrophages and lungs of mice. Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one
-
-
?
additional information
?
-
-
the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one
-
-
?
additional information
?
-
the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one
-
-
?
additional information
?
-
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
-
-
?
additional information
?
-
no activity with cholic acid, progesterone and 4-androsten-3,17-dione
-
-
?
additional information
?
-
the enzyme does not use glycerol as substrate
-
-
?
additional information
?
-
-
the enzyme does not use glycerol as substrate
-
-
?
additional information
?
-
-
the enzyme shows substrate specificity for 3beta-hydroxysteroids. No activity with cholic acid and cholesteryl linoleate
-
-
?
additional information
?
-
the enzyme does not use glycerol as substrate
-
-
?
additional information
?
-
-
the enzyme shows substrate specificity for 3beta-hydroxysteroids. No activity with cholic acid and cholesteryl linoleate
-
-
?
additional information
?
-
no activity with cholic acid, progesterone and 4-androsten-3,17-dione
-
-
?
additional information
?
-
-
no activity with ergosterol
-
-
?
additional information
?
-
-
cholesterol oxidase is involved in pimaricin biosynthesis
-
-
?
additional information
?
-
-
no activity with cholest-5-en-3alpha-ol
-
-
?
additional information
?
-
-
no activity with cholest-5-en-3alpha-ol
-
-
?
additional information
?
-
-
the wild-type enzyme also shows cholesterol dehydrogenase activity with 0.001 U/mg
-
-
?
additional information
?
-
-
the enzyme catalyzes the oxidation of steroid substrates which have a hydroxyl group at the 3beta-position of the steroid ring system
-
-
?
additional information
?
-
-
cholesterol oxidase is a bifunctional FAD-containing oxidoreductase which catalyzes the oxidation and isomerization of cholesterol into 4-cholesten-3-one
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional flavoenzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one
-
-
?
additional information
?
-
the enzyme catalyzes the oxidation and isomerization of steroids containing a 3-hydroxyl group, with the cholestane group conferring binding specificity in the active site. The enzyme is also able to oxidize small-molecule alcohols such as 2-propanol and methanol, with a preference for those containing aromatic ring, but with lower activity. Substrate oxidation results in reduction of the cofactor during the reductive half reaction. An oxidative half reaction occurs with re-oxidation of the flavin cofactor by molecular oxygen to form hydrogen peroxide. Structure-function relationship and analysis overview
-
-
?
additional information
?
-
-
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
-
-
?
additional information
?
-
-
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3beta-chlorocholest-5-ene + O2
? + H2O2
low activity
-
-
?
androst-4-ene-3,17-dione + O2
? + H2O2
-
-
-
?
beta-sitosterol + O2
? + H2O2
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
cholest-4-en-3-one + O2
? + H2O2
-
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
cholesterol + O2
cholest-4-en-3-one + H2O2
cholesterol + O2
cholest-5-en-3-one + H2O2
dehydroandrosterone + O2
? + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
diosgenin + O2
? + H2O2
-
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
pregnenolone + O2
? + H2O2
-
-
-
?
additional information
?
-
beta-cholestanol + O2
?
13% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
69% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
95% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
78% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
78% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
75% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
101% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
Streptoverticillium cholesterolieum
-
96% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
79% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
79% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
20% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
84% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
84% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
80% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
50% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
72% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Streptoverticillium cholesterolieum
-
39% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
10% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
59% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
36% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Corynebacterium cholesterolicum
-
12% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
47% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
40% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
40% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
37% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
50% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Streptoverticillium cholesterolieum
-
17% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% activity compared to cholesterol
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Corynebacterium cholesterolicum
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
cholesterol oxidase is required for the biosynthesis of the antifungal polyene pimaricin
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
cholesterol oxidase is required for the biosynthesis of the antifungal polyene pimaricin, apparently acting as an antifungal sensor
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
via cholest-5-en-3-one
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
via cholest-5-en-3-one
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Streptoverticillium cholesterolieum
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
r
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
41% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
16% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
16% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
27% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Corynebacterium cholesterolicum
-
5% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
28% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
24% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
37% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Streptoverticillium cholesterolieum
-
48% activity compared to cholesterol
-
-
?
epiandrosterone + O2
?
10% activity compared to cholesterol
-
-
?
epiandrosterone + O2
?
15% activity compared to cholesterol
-
-
?
epiandrosterone + O2
?
-
1% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
20% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
20% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Corynebacterium cholesterolicum
-
4% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
17% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
51% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
51% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
14% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Streptoverticillium cholesterolieum
-
5% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
22% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
32% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
32% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
95% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Corynebacterium cholesterolicum
-
38% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
6% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
20% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
99% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
42% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Streptoverticillium cholesterolieum
-
37% activity compared to cholesterol
-
-
?
additional information
?
-
no activity with ergosterol
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
-
intact ChoD is important for the growth of Mycobacterium tuberculosis in peritoneal macrophages and lungs of mice. Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis
-
-
?
additional information
?
-
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
-
-
?
additional information
?
-
-
no activity with ergosterol
-
-
?
additional information
?
-
-
cholesterol oxidase is involved in pimaricin biosynthesis
-
-
?
additional information
?
-
-
cholesterol oxidase is a bifunctional FAD-containing oxidoreductase which catalyzes the oxidation and isomerization of cholesterol into 4-cholesten-3-one
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional flavoenzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one
-
-
?
additional information
?
-
-
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
-
-
?
additional information
?
-
-
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
-
-
?
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0.0031
17beta-Hydroxymethyl-5-androsten-3beta-ol
Nocardia erythropolis
-
-
0.0071
20,22-dihydroxycholesterol
Nocardia erythropolis
-
-
0.124
20,25-Diazacholesterol
Nocardia erythropolis
-
-
0.0042
20-hydroxycholesterol
Nocardia erythropolis
-
-
0.0051
22-hydroxycholesterol
Nocardia erythropolis
-
-
0.0093
23,24-dinor-5-cholene-3beta,22-diol
Nocardia erythropolis
-
-
0.0043
24-hydroxycholesterol
Nocardia erythropolis
-
-
0.0027
24-oxocholesterol
Nocardia erythropolis
-
-
0.0014
25-Hydroxy-27-norcholesterol
Nocardia erythropolis
-
-
0.0015
25-hydroxycholesterol
Nocardia erythropolis
-
-
0.0012
26-Hydroxycholesterol
0.0036
3beta-hydroxy-5-androsten-17-one
Nocardia erythropolis
-
-
0.0017
3beta-hydroxy-5-pregnen-20-one
Nocardia erythropolis
-
-
0.3 - 1.2
3beta-hydroxy-androst-5-en-17-one
0.0075
4,4-Dimethylcholesterol
Nocardia erythropolis
-
-
0.0126
4alpha-Methylcholesterol
Nocardia erythropolis
-
-
0.0284
4beta-methylcholesterol
Nocardia erythropolis
-
-
0.0055
5,7-cholestadien-3beta-ol
Nocardia erythropolis
-
-
0.548
5-androstene-3,17-dione
Nocardia erythropolis
-
-
0.0048
5-Cholene-3beta,24-diol
Nocardia erythropolis
-
-
0.007 - 1.52
5-Cholesten-3-one
0.0125
5-Pregnen-3,20-dione
Nocardia erythropolis
-
-
0.0037
5-pregnene-3beta,20beta-diol
Nocardia erythropolis
-
-
0.0028
5alpha-Cholest-8(14)-en-3beta-ol
Nocardia erythropolis
-
-
0.0107
5alpha-cholest-8(14)en-3beta-ol
Nocardia erythropolis
-
-
0.0023
5alpha-Cholestan-3beta-ol
Nocardia erythropolis
-
-
0.17842 - 0.5
beta-sitosterol
0.0000081 - 45.2
cholesterol
0.2 - 0.25
dehydroandrosterone
0.0275 - 1
dehydroepiandrosterone
0.0115
fucosterol
Nocardia erythropolis
-
-
0.0000058 - 4
pregnenolone
0.0059 - 0.78961
stigmasterol
0.5
taurocholate
pH 6.75, 35°C
0.5 - 0.8
trans-androsterone
0.2 - 1.2
trans-dehydroandrosterone
additional information
additional information
-
0.0012
26-Hydroxycholesterol
Nocardia erythropolis
-
-
0.0012
26-Hydroxycholesterol
Nocardia erythropolis
-
5-androstene-3beta,17beta-diol
0.3
3beta-hydroxy-androst-5-en-17-one
-
-
0.3
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.5
3beta-hydroxy-androst-5-en-17-one
-
-
0.5
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
0.8
3beta-hydroxy-androst-5-en-17-one
-
-
0.8
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
1.2
3beta-hydroxy-androst-5-en-17-one
-
-
1.2
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.007
5-Cholesten-3-one
Nocardia erythropolis
-
-
0.27
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
1.52
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.17842
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.18
beta-sitosterol
pH 7.5, 37°C, full-length isozyme ChoM2
0.19
beta-sitosterol
pH 7.5, 37°C, truncated isozyme ChoM2
0.2
beta-sitosterol
pH 7.5, 37°C
0.22076
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.25
beta-sitosterol
-
in the presence of undecanol
0.32861
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.02
cholestanol
-
-
0.2
cholestanol
-
assay relies on detection of H2O2 formation
0.2
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.7
cholestanol
-
assay relies on detection of H2O2 formation
0.7
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.0000081
cholesterol
-
pH 7.0, 37°C, mutant enzyme S379T
0.00002
cholesterol
-
pH 7.0, 37°C, wild-type enzyme
0.00003
cholesterol
-
pH 7.0, 37°C, mutant enzyme S379A
0.000046
cholesterol
-
pH 7.0, 37°C, mutant enzyme S379V
0.000072
cholesterol
-
pH 7.0, 37°C, mutant enzyme V145Q
0.000081
cholesterol
-
pH 7.0, 37°C, mutant enzyme L119A
0.00011
cholesterol
-
pH 7.0, 37°C, mutant enzyme P357N
0.00013
cholesterol
-
pH 7.0, 37°C, mutant enzyme Q286R
0.00017
cholesterol
-
pH 7.0, 37°C, mutant enzyme L119F
0.0004
cholesterol
-
pH 7.0, 37°C, mutant enzyme V145D
0.00067
cholesterol
-
pH 7.0, 37°C, mutant enzyme V145E
0.0015
cholesterol
mutant enzyme G347N, determined by cholest-4-en-3-one detection
0.00245
cholesterol
-
recombinant mutant V191A, pH 7.0, 22°C
0.0027
cholesterol
mutant enzyme F359W, determined by cholest-4-en-3-one detection
0.0027
cholesterol
wild-type enzyme, determined by cholest-4-en-3-one detection
0.0027
cholesterol
wild-type enzyme, determined by H2O2 detection
0.0027
cholesterol
apparent value, wild type enzyme, pH 7.0, temperature not specified in the publication
0.0029
cholesterol
Nocardia erythropolis
-
-
0.003
cholesterol
-
wild-type enzyme
0.003
cholesterol
-
mutant enzyme H447Q
0.004
cholesterol
-
strain ST-200
0.004
cholesterol
mutant enzyme F359W, determined by H2O2 detection
0.0046
cholesterol
apparent value, mutant enzyme N485L, pH 7.0, temperature not specified in the publication
0.005
cholesterol
-
mutant enzyme H447Q/E361Q
0.005
cholesterol
mutant enzyme G347N, determined by H2O2 detection
0.0051
cholesterol
Nocardia erythropolis
-
-
0.0054
cholesterol
-
mutant enzyme E361
0.0062
cholesterol
mutant enzyme N485D, determined by H2O2 detection
0.0062
cholesterol
apparent value, mutant enzyme N485L, pH 5.1, temperature not specified in the publication
0.0063
cholesterol
apparent value, mutant enzyme N485D, pH 5.1, temperature not specified in the publication
0.0066
cholesterol
apparent value, mutant enzyme N485D, pH 7.0, temperature not specified in the publication
0.0067
cholesterol
apparent value, wild type enzyme, pH 5.1, temperature not specified in the publication
0.007
cholesterol
mutant enzyme N485D, determined by cholest-4-en-3-one detection
0.01
cholesterol
pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate
0.011
cholesterol
-
V145E mutant
0.011
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate
0.011
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate
0.011
cholesterol
pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate
0.012
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate
0.013
cholesterol
-
wild-type
0.013
cholesterol
Nocardia erythropolis
-
Km increases when enzyme is entrapped in reverse micelles of the synthetic surfactant aerosol-OT-isooctane
0.013
cholesterol
-
S103T mutant
0.013
cholesterol
pH 7, 25°C
0.013
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate
0.013
cholesterol
pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate
0.014
cholesterol
pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate
0.017
cholesterol
pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate
0.0184
cholesterol
-
pH 7.0, temperature not specified in the publication
0.0188
cholesterol
pH and temperature not specified in the publication
0.0221
cholesterol
pH 7.0, 30°C
0.0226
cholesterol
pH 7.0, 30°C
0.026
cholesterol
-
pH 6.0 or pH 7.0
0.0262
cholesterol
pH and temperature not specified in the publication
0.03
cholesterol
-
R135H mutant
0.03
cholesterol
-
pH 7.0, 37°C, cholesterol oxidase II
0.031
cholesterol
-
cell-linked and extracellular enzyme
0.031
cholesterol
pH 7.0, 25°C
0.032
cholesterol
pH 7.0, 50°C
0.04
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.043
cholesterol
-
pH 7.0, 37°C
0.07
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.0926
cholesterol
pH 7.0-8.0, 30°C
0.0962
cholesterol
-
enzyme from cultured immobilized cells, pH and temperature not specified in the publication
0.1013
cholesterol
-
pH 7.0, 37°C
0.1013
cholesterol
-
enzyme from cultured free cells, pH and temperature not specified in the publication
0.11
cholesterol
-
pH 8.0, 37°C
0.11
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.12
cholesterol
pH 7.5, 37°C, full-length isozyme ChoM2
0.12
cholesterol
-
recombinant wild-type enzyme, pH 7.0, 22°C
0.13
cholesterol
pH 7.5, 40°C, recombinant isozyme ChoM1
0.14
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.14
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.14
cholesterol
pH 7.5, 37°C, truncated isozyme ChoM2
0.152
cholesterol
-
pH 7.0, 37°C
0.15483
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.16
cholesterol
-
pH 8.0, 40°C
0.16
cholesterol
pH 7.5, 40°C, recombinant isozyme ChoM1
0.17
cholesterol
pH 7.5, 37°C
0.17
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.17831
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.2
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.2
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.2
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.20485
cholesterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.2123
cholesterol
-
apparent value, free enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C
0.25
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.25
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.2599
cholesterol
-
apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C
0.4
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.47
cholesterol
-
H69A mutant enzyme, 50 mM phosphate buffer, pH 7.5, 1% thesit, 1% 2-propanol
0.524
cholesterol
pH 7.5, 37°C
0.54
cholesterol
-
pH 9.0, 37°C
0.556
cholesterol
-
pH 7.0, 37°C
0.8
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1
cholesterol
-
in the presence of undecanol
1.1
cholesterol
-
pH 7.0, 37°C, cholesterol oxidase I
2
cholesterol
-
in the presence of undecanol
3.25
cholesterol
-
pH 7.5, 37°C
4.5
cholesterol
-
pH 7, 30°C, enzyme CO2
6.76
cholesterol
-
pH 7, 30°C, enzyme CO1
24.3
cholesterol
-
native enzyme, pH 7.2, 37°C
25.4
cholesterol
-
non-tagged recombinant enzyme, pH 7.2, 37°C
35.4
cholesterol
-
N-terminally His-tagged recombinant enzyme , pH 7.2, 37°C
45.2
cholesterol
-
C-terminally His-tagged recombinant enzyme , pH 7.2, 37°C
0.2
dehydroandrosterone
pH 7.5, 37°C, full-length isozyme ChoM2
0.24
dehydroandrosterone
pH 7.5, 37°C
0.25
dehydroandrosterone
pH 7.5, 37°C, truncated isozyme ChoM2
0.0275
dehydroepiandrosterone
-
0.27352
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.31881
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.34121
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.4
dehydroepiandrosterone
-
-
0.491
dehydroepiandrosterone
pH 7.5, 37°C
1
dehydroepiandrosterone
-
in the presence and absence of undecanol
0.195
diosgenin
pH 7.5, 37°C
0.23
diosgenin
pH 7.5, 37°C
0.23
diosgenin
pH 7.5, 37°C, full-length isozyme ChoM2
0.26
diosgenin
pH 7.5, 37°C, truncated isozyme ChoM2
0.165
O2
-
-
0.45
O2
-
H69A mutant enzyme, 50 mM phosphate buffer, pH 7.5, 1% thesit, 1% 2-propanol
0.617
O2
mutant enzyme F359W
0.0000058
pregnenolone
-
pH 7.0, 37°C, mutant enzyme S379T
0.000023
pregnenolone
-
pH 7.0, 37°C, wild-type enzyme
0.000023
pregnenolone
-
pH 7.0, 37°C, mutant enzyme S379A
0.00004
pregnenolone
-
pH 7.0, 37°C, mutant enzyme L119A
0.00004
pregnenolone
-
pH 7.0, 37°C, mutant enzyme S379V
0.000056
pregnenolone
-
pH 7.0, 37°C, mutant enzyme Q286R
0.000058
pregnenolone
-
pH 7.0, 37°C, mutant enzyme V145Q
0.000077
pregnenolone
-
pH 7.0, 37°C, mutant enzyme L119F
0.000083
pregnenolone
-
pH 7.0, 37°C, mutant enzyme V145E
0.000098
pregnenolone
-
pH 7.0, 37°C, mutant enzyme P357N
0.0001
pregnenolone
-
pH 7.0, 37°C, mutant enzyme V145D
0.19061
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.2
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.2
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.2
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.2
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.22842
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.29455
pregnenolone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.33
pregnenolone
pH 7.5, 37°C, full-length isozyme ChoM2
0.35
pregnenolone
pH 7.5, 37°C, truncated isozyme ChoM2
0.38
pregnenolone
pH 7.5, 37°C
0.4
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.4
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
4
pregnenolone
-
in the presence of undecanol
0.0066
sitosterol
Nocardia erythropolis
-
-
0.59 - 1
sitosterol
pH 7.5, 37°C
0.0059
stigmasterol
Nocardia erythropolis
-
-
0.38412
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.52884
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.78961
stigmasterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.5
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.8
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.3
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.9
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
additional information
additional information
-
kinetics
-
additional information
additional information
-
Km-values of enzyme immobilized on platinum electrode modified with thiolipid/lipid bilayer membranes
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics, modelling
-
additional information
additional information
-
kinetics, modelling
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.8
3beta-hydroxy-5-androsten-17-one
0.8 - 8.2
3beta-hydroxy-androst-5-en-17-one
28 - 670
5-Cholesten-3-one
9.86 - 16.06
beta-sitosterol
0.69 - 13.68
dehydroepiandrosterone
7
epiandrosterone
-
H2O2 production assayed, 100 mM phosphate buffer
0.8 - 7
trans-androsterone
0.8 - 8.2
trans-dehydroandrosterone
0.8
3beta-hydroxy-5-androsten-17-one
-
assay relies on detection of H2O2 formation
0.8
3beta-hydroxy-5-androsten-17-one
-
H2O2 production assayed, 100 mM phosphate buffer
0.8
3beta-hydroxy-androst-5-en-17-one
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
7
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
8.2
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
8.2
3beta-hydroxy-androst-5-en-17-one
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
28
5-Cholesten-3-one
-
H69A mutant enzyme, 5-cholesten-3-one isomerization, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
250
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
278
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
330
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
332
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
670
5-Cholesten-3-one
-
isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 50 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol
9.86
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
13.25
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
16.06
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
37
cholestanol
-
assay relies on detection of H2O2 formation
37
cholestanol
-
H2O2 production assayed, 100 mM phosphate buffer
37
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
40
cholestanol
-
assay relies on detection of H2O2 formation
40
cholestanol
-
H2O2 production assayed, 100 mM phosphate buffer
40
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.0014
cholesterol
-
mutant enzyme H447E/E361Q
0.017
cholesterol
-
mutant enzyme H447E
0.035
cholesterol
mutant enzyme N485D, determined by H2O2 detection
0.035
cholesterol
apparent value, mutant enzyme N485L, pH 5.1, temperature not specified in the publication
0.044
cholesterol
apparent value, mutant enzyme N485L, pH 7.0, temperature not specified in the publication
0.073
cholesterol
mutant enzyme N485D, determined by cholest-4-en-3-one detection
0.073
cholesterol
apparent value, mutant enzyme N485D, pH 7.0, temperature not specified in the publication
0.093
cholesterol
-
mutant enzyme H447Q/E361Q
0.32
cholesterol
-
mutant enzyme H447Q
0.55
cholesterol
-
H69A mutant enzyme, steady state, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
0.61
cholesterol
pH 7.0, 30°C
0.8
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation, decreasing length of C17 chain affects turnover negatively
0.85
cholesterol
mutant enzyme G347N, determined by H2O2 detection
0.86
cholesterol
mutant enzyme F359W, determined by cholest-4-en-3-one detection
1
cholesterol
mutant enzyme G347N, determined by cholest-4-en-3-one detection
1.3
cholesterol
mutant enzyme F359W, determined by H2O2 detection
1.4
cholesterol
-
mutant enzyme E361
1.4
cholesterol
apparent value, mutant enzyme N485D, pH 5.1, temperature not specified in the publication
1.6
cholesterol
-
H69A mutant enzyme, cholesterol oxidation, 50 mM phosphate buffer, 1% thesit, 1% 2-propanol
2.21
cholesterol
-
recombinant mutant V191A, pH 7.0, 22°C
3
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
6
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
6.8
cholesterol
pH 7.0, 30°C
9
cholesterol
-
pH 7.0, 37°C, mutant enzyme P357N
9
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
10.6
cholesterol
-
pH 8.0, 40°C
11
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
11
cholesterol
-
pH 7.0, 37°C, mutant enzyme V145D
11
cholesterol
-
pH 7.0, 37°C, mutant enzyme V145Q
11
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
12.29
cholesterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
15
cholesterol
-
pH 7.0, 37°C, mutant enzyme L119A
15.48
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
23
cholesterol
-
pH 7.0, 37°C, mutant enzyme Q286R
23
cholesterol
-
pH 7.0, 37°C, mutant enzyme V145E
23
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate
23.02
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
27
cholesterol
-
pH 7.0, 37°C, mutant enzyme L119F
28
cholesterol
-
50 mM phosphate buffer, pH 7.5
29
cholesterol
-
pH 7.0, 37°C, mutant enzyme S379T
30
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate
32
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
40
cholesterol
-
pH 7.0, 37°C, wild-type enzyme
42
cholesterol
wild-type enzyme, determined by cholest-4-en-3-one detection
43
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
44
cholesterol
-
wild-type enzyme
46
cholesterol
apparent value, wild type enzyme, pH 5.1, temperature not specified in the publication
46
cholesterol
pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate
47
cholesterol
wild-type enzyme, determined by H2O2 detection
47
cholesterol
apparent value, wild type enzyme, pH 7.0, temperature not specified in the publication
47.3
cholesterol
-
recombinant wild-type enzyme, pH 7.0, 22°C
48
cholesterol
-
50 mM phosphate buffer, pH 7.5
48
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
48
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
48.1
cholesterol
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
49
cholesterol
-
pH 7.0, 37°C, mutant enzyme S379A
51
cholesterol
-
pH 7.0, 37°C, mutant enzyme S379V
56
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
57
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
57
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate
60
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate
63
cholesterol
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
63
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
67
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
67
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
67
cholesterol
pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate
68
cholesterol
pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate
75
cholesterol
pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate
89
cholesterol
pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate
105
cholesterol
-
50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol
345
cholesterol
-
50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol
0.69
dehydroepiandrosterone
-
6.82
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
9.56
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
13.68
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
9
pregnenolone
-
pH 7.0, 37°C, mutant enzyme P357N
9
pregnenolone
-
pH 7.0, 37°C, mutant enzyme V145D
11
pregnenolone
-
pH 7.0, 37°C, mutant enzyme V145E
11.78
pregnenolone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
12
pregnenolone
-
pH 7.0, 37°C, mutant enzyme V145Q
13
pregnenolone
-
pH 7.0, 37°C, mutant enzyme L119A
13.71
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
15.25
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
19
pregnenolone
-
pH 7.0, 37°C, mutant enzyme Q286R
21
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
21
pregnenolone
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
21
pregnenolone
-
pH 7.0, 37°C, mutant enzyme L119F
21
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
21
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
24
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
24
pregnenolone
-
product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer
24
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
30
pregnenolone
-
pH 7.0, 37°C, wild-type enzyme
35
pregnenolone
-
pH 7.0, 37°C, mutant enzyme S379A
35
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
39
pregnenolone
-
pH 7.0, 37°C, mutant enzyme S379V
45
pregnenolone
-
pH 7.0, 37°C, mutant enzyme S379T
0.08
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
1.58
stigmasterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
4.23
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.8
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
7
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.8
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
6
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
8.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
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synthesis
the enzyme is used for the production of steroid drugs and insecticides
agriculture
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strain A19249, enzyme exhibits a potent insecticidal activity
agriculture
-
recombinant expression in plants gives insect resistance
agriculture
-
recombinant expression in plants gives insect resistance
agriculture
Nocardia rhodochrous
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recombinant expression in plants gives insect resistance
agriculture
Nocardia erythropolis
-
recombinant expression in plants gives insect resistance
agriculture
-
recombinant expression in plants gives insect resistance
agriculture
-
recombinant expression in plants gives insect resistance
agriculture
Pseudomonas spp.
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recombinant expression in plants gives insect resistance
agriculture
-
recombinant expression in plants gives insect resistance
agriculture
-
recombinant expression in plants gives insect resistance
agriculture
Streptoverticillum cholesterolicum
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recombinant expression in plants gives insect resistance
agriculture
recombinant expression in plants gives insect resistance
agriculture
-
strain A19249, enzyme exhibits a potent insecticidal activity
-
agriculture
-
recombinant expression in plants gives insect resistance
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analysis
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platinum electrodes modified with thiolipid/lipid bilayer membranes. Cholesterol oxidase spontaneously inserts into the electrode-supported lipid bilayer membrane from solution and is consequently immobilized to the electrode surface. This electrode architecture may be useful in clinical sensing applications
analysis
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the cholesterol oxidase-immobilized polyacrylonitrile hollow fiber may have an industrial or medical application for cholesterol determination or oxidation
analysis
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the enzyme can be used as cholesterol biosensor co-immobilized with cholesterol esterase on oxygen electrodes, determination of total cholesterol in food samples
analysis
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Cellulomonas enzyme is analytically reliable when used for serum cholesterol determination by the endpoint method. Its analytical performance is equivalent to Streptomyces enzymes and meets the analytical goals. It has an advantage over the other enzymes in that it does not ship in the frozen state
analysis
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cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) can be immobilized onto tetraethylorthosilicate derived sol-gel films. These tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films can be used for the estimation of cholesterol oleate up to 780 mg/dl (mM). These TEOS sol-gel enzyme films provide selectivity towards glucose, lactate, uric acid and ascorbic acid
analysis
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simple, reliable, fast and reproducible method to prepare cholesterol oxidase electrodes is described
analysis
-
the steady state amperometric measurements of free cholesterol are performed by an enzyme electrode which is developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF+ClO4-, film that is coated on a Pt electrode
analysis
-
the development of an enzyme-based sensor employing the enzyme ChOx has great potential as a simple and economical sensor system, engineering of the enzyme for electrochemical monitoring of cholesterol, overview
biotechnology
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biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Nocardia rhodochrous
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Nocardia erythropolis
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Pseudomonas spp.
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
Streptoverticillum cholesterolicum
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
biotechnology
-
Cholesterol oxidase has two major biotechnological applications, i.e. in the determination of serum (and food) cholesterol levels and as biocatalyst providing valuable intermediates for industrial steroid drug production
biotechnology
-
biocatalysis, industrial steroid drug production, steroid production as diagnostic tool
-
diagnostics
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the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium
diagnostics
-
the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium
diagnostics
-
analytic tool for determining cholesterol in various samples
diagnostics
-
analytic tool for determining cholesterol in various samples
diagnostics
Nocardia rhodochrous
-
analytic tool for determining cholesterol in various samples
diagnostics
Nocardia erythropolis
-
analytic tool for determining cholesterol in various samples
diagnostics
-
analytic tool for determining cholesterol in various samples
diagnostics
-
analytic tool for determining cholesterol in various samples
diagnostics
Pseudomonas spp.
-
analytic tool for determining cholesterol in various samples
diagnostics
-
analytic tool for determining cholesterol in various samples
diagnostics
-
analytic tool for determining cholesterol in various samples
diagnostics
Streptoverticillum cholesterolicum
-
analytic tool for determining cholesterol in various samples
diagnostics
analytic tool for determining cholesterol in various samples
diagnostics
-
the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase
diagnostics
-
determination of cholesterol, by rapid, cheap and reliable methods, is very important in clinical diagnosis because its level in blood is closely related to human health. A reproducible cholesterol biosensors is prepared based on the direct adsorption of ChOx onto gold substrates (ChOx-Au)
diagnostics
the enzyme is an important biotechnological tool for clinical diagnostics. It is also used for tracking intracellular cholesterol. Its utility is limited by the lack of an efficient temporal control of its activity. A rapamycin-inducible fluorescent cholesterol oxidase from Chromobacterium sp. DS-1 (split GFP-COase) is engineered. Induction by rapamycin allows temporal control over enzyme activity and rapidly leads to an efficient reduction of intracellular cholesterol. It is proposed that this drug-inducible split GFP-COase is a new tool to manipulate the cellular cholesterol content and gain insights into cholesterol-dependent proteins and cholesterol-related cell pathologies
diagnostics
-
analytic tool for determining cholesterol in various samples
-
diagnostics
-
the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase
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drug development
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the cytotoxicity activity against a few cancer cell lines, while broadly sparing the normal cells, suggests the possible use of this enzyme as a promising future contender for anticancer therapy
drug development
-
the enzyme showed significant cytotoxicity on breast (MCF-7), nasopharyngeal (KB) and ovarian (OVCAR) cancer cell lines at very low concentrations ranging from 0.000093 to 0.00014 mM. It exhibits relatively less cytotoxicity on primary mouse embryonic fibroblast (3T3) cells. Thus, cholesterol oxidase from Streptomyces sp. AKHSS could be a potential anticancer agent
medicine
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determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Nocardia rhodochrous
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Nocardia erythropolis
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
Corynebacterium cholesterolicum
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
medicine
-
determination of cholesterol in various sample types, determination of cholesterol in gall stones, determination of cholesterol on the cell membrane of erythrocytes, other cells and cellular compartments
-