Information on EC 1.1.99.14 - glycolate dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.99.14
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RECOMMENDED NAME
GeneOntology No.
glycolate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycolate + acceptor = glyoxylate + reduced acceptor
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycolate and glyoxylate degradation I
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glycolate and glyoxylate degradation II
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glycolate and glyoxylate degradation III
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glycolate and glyoxylate degradation
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Glyoxylate and dicarboxylate metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
glycolate:acceptor 2-oxidoreductase
Also acts on (R)-lactate. 2,6-Dichloroindophenol and phenazine methosulfate can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
37368-32-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 183.80
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Manually annotated by BRENDA team
strain 183.80
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Manually annotated by BRENDA team
strain K12
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Manually annotated by BRENDA team
strain K12
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Manually annotated by BRENDA team
Z
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Manually annotated by BRENDA team
Z
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Manually annotated by BRENDA team
strain 1405-1
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Manually annotated by BRENDA team
strain PCC 6803, gene glcD
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Manually annotated by BRENDA team
Cyclotella nana; Guillard strain 3H
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-lactate + acceptor
pyruvate + reduced acceptor
show the reaction diagram
(S)-lactate + acceptor
pyruvate + reduced acceptor
show the reaction diagram
2,6-dichlorophenol-indophenol + acceptor
?
show the reaction diagram
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DCIP decoloration assay, mitochondrial glycolate oxidation contributes to photorespiration in higher plants
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-
?
dihydroxyacetone + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
show the reaction diagram
DL-glyceraldehyde + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
show the reaction diagram
DL-lactate + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
show the reaction diagram
DL-malate + acceptor
2-oxosuccinate + reduced acceptor
show the reaction diagram
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about 3% activity of glycolate
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?
glycolaldehyde + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
show the reaction diagram
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38% activity compared to glycolate
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?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
glycolate + acceptor
glyocylate + reduced acceptor
show the reaction diagram
glycolate + acceptor
glyoxylate + reduced acceptor
show the reaction diagram
glycolate + NAD+
glyoxylate + NADH
show the reaction diagram
glycolate + nitro blue tetrazolium
glyoxylate + reduced nitro blue tetrazolium
show the reaction diagram
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100% activity
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?
glyoxylate + acceptor
oxalate + reduced acceptor
show the reaction diagram
L-lactate + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
show the reaction diagram
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232% activity compared to glycolate
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?
methyl glycolate + nitro blue tetrazolium
methyl glyoxylate + reduced nitro blue tetrazolium
show the reaction diagram
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82% activity compared to glycolate
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?
phosphoglycolate + acceptor
? + reduced acceptor
show the reaction diagram
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about 9% activity of glycolate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycolate + acceptor
glyocylate + reduced acceptor
show the reaction diagram
glycolate + acceptor
glyoxylate + reduced acceptor
show the reaction diagram
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involved in the photorespiration pathway
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?
glycolate + NAD+
glyoxylate + NADH
show the reaction diagram
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the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
cytochrome c
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can replace dichlorophenolindophenol as acceptor
phenazine methosulfate
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-oxyquinoline
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80% residual activity at 1 mM
Aminooxyacetate
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competitive to glycolate and glyoxylate
Co2+
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88% residual activity at 1 mM
crude oil
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33-54% inhibition at 1-2 ml l-1, complete inhibition at 4 ml l-1
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Cu2+
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complete inhibition at 1 mM
Cyclohexane
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33-68% inhibition at 0.01-0.03% (v/v), complete inhibition at 0.04% (v/v)
cytochrome c
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inhibits rate of phenazine methosulfate stimulated dichlorophenolindophenol reduction
DW 1750000
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91% residual activity at 1 mM
Fe3+
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73% residual activity at 1 mM
glyoxylate
Hg2+
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2% residual activity at 1 mM
HgCl2
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complete
hydrazine
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77% residual activity at 1 mM
hydroxylamine
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83% residual activity at 1 mM
iodoacetic acid
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63% residual activity at 1 mM
K3[Fe(CN)6]
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complete inhibition
Mn2+
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complete inhibition at 1 mM
N-ethylmaleimide
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56% residual activity at 1 mM
Ni2+
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complete inhibition at 1 mM
o-phenanthroline
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88% residual activity at 1 mM
Sodium azide
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85% residual activity at 1 mM
Zn2+
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76% residual activity at 1 mM
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dodecane
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65% increase of activity at 1.75% (v/v)
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7 - 28
D(-)-lactate
49.5
dihydroxyacetone
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at pH 7.0 and 30C
0.04 - 16
glycolate
22
L(+)-lactate
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3.6
L-lactate
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at pH 7.0 and 30C
0.15
Nitro blue tetrazolium
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at pH 7.0 and 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
Aminooxyacetate
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0.01
glyoxylate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00165
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crude cell extract, at pH 7.0 and 30C
0.005
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N52 mutant
0.015
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at 400 ppm CO2
0.035
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at 100 ppm CO2
0.14
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glyoxylate formation in the presence of phenazine methosulfate
0.15
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crude extract
0.727
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after 440fold purification, at pH 7.0 and 30C
3.1
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partially purified protein
additional information
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determination of internal glycolate concentrations
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 9
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about half-maximal activity at pH 7.3 and 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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by differential centrifugation and sucrose density gradient centrifugation
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
280000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.5
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more than 80% of enzyme activity remains in the pH range between 5.0 and 6.5 for 20 min at 20C
725738
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
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when the enzyme is incubated with 0.1 M potassium phosphate buffer, pH 6.0, at 2060C for 30 min, approximately 90% of the enzyme activity remain below 20C, but the activity completely disappears at 40C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2C, half-life of 10 days, FMN or FAD do not restore activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-Toyopearl column chromatography, Q-Toyopearl column chromatography, and Toyopearl, GigaCap Q-Toyopearl column chromatography, hydroxyapatite column chromatography, and gel filtration
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partially, from cells grown on glycolate
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recombinant N-terminally Strep-tagged enzyme from Escherichia coli strain BL21 by streptavidin-tactin affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complementation of glycolate dehydrogenase deficient Escherichia coli
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gene glcD, expression of N-terminally Strep-tagged enzyme in Escherichia coli strain BL21
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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construction of GlcD-deficient mutants, co-mutation of other enzymes of the alternative bacterial glycerate pathway, progressive segregation, genotyping and metabolic analysis, growth phenotypes under normal and carbon limited conditions, overview
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