Information on EC 1.1.99.30 - 2-oxo-acid reductase

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The expected taxonomic range for this enzyme is: Proteus

EC NUMBER
COMMENTARY hide
1.1.99.30
-
RECOMMENDED NAME
GeneOntology No.
2-oxo-acid reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a (2R)-hydroxy-carboxylate + acceptor = a 2-oxo-carboxylate + reduced acceptor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
redox reaction
-
-
reduction
-
-
SYSTEMATIC NAME
IUBMB Comments
(2R)-hydroxy-carboxylate:acceptor oxidoreductase
Contains [4Fe-4S] and a mononucleotide molybdenum (pyranopterin) cofactor. Has broad substrate specificity, with 2-oxo-monocarboxylates and 2-oxo-dicarboxylates acting as substrates. Branching in a substrate at the C-3 position results in loss of activity. The enzyme from Proteus sp. is inactivated by oxygen.
CAS REGISTRY NUMBER
COMMENTARY hide
70852-00-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-hydroxy-4-methylpentanoate + 2-oxo-butyrate
2-oxo-4-methylpentanoate + (2R)-hydroxybutyrate
show the reaction diagram
-
-
-
-
r
(2S,3R,4S)-2-hydroxy-3-halogenbutyrolactones + reduced benzyl viologen
?
show the reaction diagram
-
highly stereospecific reaction
-
-
r
(2S,3R,4S)-2-hydroxy-3-halogenbutyrolactones + reduced methyl viologen
?
show the reaction diagram
-
highly stereospecific reaction
-
-
r
(R)-lactate + oxidized anthraquinone-2,6-disulfonate
pyruvate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
?
(R,S)-2-oxo-3-methylpentanoate + reduced benzyl viologen
2-hydroxy-3-methylpentanoate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
-
(R,S)-2-oxo-3-methylpentanoate + reduced methyl viologen
2-hydroxy-3-methylpentanoate + oxidized methyl viologen
show the reaction diagram
-
-
-
-
-
(S)-2-oxo-3-methylpentanoate + reduced benzyl viologen
2-hydroxy-3-methylpentanoate + oxidized benzyl viologen
show the reaction diagram
(S)-2-oxo-3-methylpentanoate + reduced methyl viologen
2-hydroxy-3-methylpentanoate + oxidized methyl viologen
show the reaction diagram
2-hydroxyglutarate + oxidized acceptor
2-oxoglutarate + reduced acceptor
show the reaction diagram
-
-
-
-
r
2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced benzyl viologen
3,3-dimethyl-2,4-dihydroxybutanoate + oxidized benzyl viologen
show the reaction diagram
2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced methyl viologen
3,3-dimethyl-2,4-dihydroxybutanoate + oxidized methyl viologen
show the reaction diagram
2-oxo-3,5-dienoates + H2
?
show the reaction diagram
-
-
-
-
r
2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2
rosmarinic acid
show the reaction diagram
-
-
-
-
r
2-oxo-3-enoates + H2
?
show the reaction diagram
-
-
-
-
r
2-oxo-4-(hydroxy-methyl-phosphinyl)-butanoate + reduced benzyl viologen
2-hydroxy-4-(hydroxymethylphosphinyl)-butanoate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
-
2-oxo-4-(hydroxy-methyl-phosphinyl)-butanoate + reduced methyl viologen
2-hydroxy-4-(hydroxymethylphosphinyl)-butanoate + oxidized methyl viologen
show the reaction diagram
-
-
-
-
-
2-oxo-4-methylpentanoate + reduced benzyl viologen
2-hydroxy-4-methylpentanoate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
-
2-oxo-4-methylpentanoate + reduced methyl viologen
2-hydroxy-4-methylpentanoate + oxidized methyl viologen
show the reaction diagram
-
-
-
-
-
2-oxo-carboxylate + reduced benzyl viologen
(2R)-hydroxy-carboxylate + oxidized benzyl viologen
show the reaction diagram
-
stereospecific, very wide substrate specificity, reduces 2-oxo-carboxylates with diverse types of residues at position 3, such as unbranched and branched alkyl, hydroxy, one or two conjugated carbon carbon double bonds, additional CO, and -OOC(CH2)n, overview
-
-
r
2-oxo-carboxylate + reduced methyl viologen
(2R)-hydroxy-carboxylate + oxidized methyl viologen
show the reaction diagram
-
stereospecific, very wide substrate specificity, reduces 2-oxo-carboxylates with diverse types of residues at position 3, such as unbranched and branched alkyl, hydroxy, one or two conjugated carbon carbon double bonds, additional CO, and -OOC(CH2)n, overview
-
-
r
2-oxoadipate + reduced benzyl viologen
2-hydroxyadipate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
-
2-oxoadipate + reduced methyl viologen
2-hydroxyadipate + oxidized methyl viologen
show the reaction diagram
-
-
-
-
-
2-oxoglutarate + reduced benzyl viologen
2-hydroxyglutarate + oxidized benzyl viologen
show the reaction diagram
2-oxoglutarate + reduced methyl viologen
2-hydroxyglutarate + oxidized methyl viologen
show the reaction diagram
2-oxononanoate + reduced benzyl viologen
2-hydroxynonanoate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
-
2-oxononanoate + reduced methyl viologen
2-hydroxynonanoate + oxidized methyl viologen
show the reaction diagram
-
-
-
-
-
3-fluoropyruvate + reduced benzyl viologen
3-fluoro-2-hydroxypropionate + oxidized benzyl viologen
show the reaction diagram
3-fluoropyruvate + reduced methyl viologen
3-fluoro-2-hydroxypropionate + oxidized methyl viologen
show the reaction diagram
5-benzyloxyindolylpyruvate + reduced benzyl viologen
3-(5-benzyloxyindolyl)-2-hydroxypropionate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
-
5-benzyloxyindolylpyruvate + reduced methyl viologen
3-(5-benzyloxyindolyl)-2-hydroxypropionate + oxidized methyl viologen
show the reaction diagram
-
-
-
-
-
6-phospho-D-gluconate + oxidized anthraquinone-2,6-disulfonate
6-phospho-D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + oxidized benzyl viologen
6-phospho-D-arabino-hex-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + oxidized carboxamido-methyl viologen
6-phospho-D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
a (2R)-hydroxy-carboxylate + acceptor
a 2-oxo-carboxylate + reduced acceptor
show the reaction diagram
chloropyruvic acid + reduced 1,1'-carbamoyl methyl viologen
D-(S)-chlorolactate + oxidized 1,1'-carbamoyl methyl viologen
show the reaction diagram
-
stereospecific reaction
-
-
?
D-galactonate + oxidized anthraquinone-2,6-disulfonate
D-lyxo-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-galactonate + oxidized benzyl viologen
D-lyxo-hex-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-galactonate + oxidized carboxamido-methyl viologen
D-lyxo-hex-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
D-glucarate + oxidized anthraquinone-2,6-disulfonate
2-oxo-D-glucarate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-glucarate + oxidized benzyl viologen
2-oxo-D-glucarate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-glucarate + oxidized carboxamido-methyl viologen
2-oxo-D-glucarate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
D-gluconate + oxidized 1,1'-carbamoyl methyl viologen
? + reduced 1,1'-carbamoyl methyl viologen
show the reaction diagram
-
anaerobic conditions
-
-
r
D-gluconate + oxidized anthraquinone-2,6-disulfonate
D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-gluconate + oxidized benzyl viologen
D-arabino-hex-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-gluconate + oxidized carboxamido-methyl viologen
D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen
? + reduced 1,1'-carbamoyl methyl viologen
show the reaction diagram
-
anaerobic conditions
-
-
r
D-gulonate + oxidized anthraquinone-2,6-disulfonate
D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-gulonate + oxidized benzyl viologen
D-arabino-hex-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-gulonate + oxidized carboxamido-methyl viologen
D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
D-lactate + oxidized 1,1'-carbamoyl methyl viologen
pyruvate + reduced 1,1'-carbamoyl methyl viologen
show the reaction diagram
-
anaerobic conditions
-
-
r
D-lactate + oxidized anthraquinone-2,6-disulfonate
pyruvate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-lactate + oxidized benzyl viologen
pyruvate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-lactate + oxidized carboxamido-methyl viologen
pyruvate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
D-ribonate + oxidized anthraquinone-2,6-disulfonate
D-erythro-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-ribonate + oxidized benzyl viologen
D-erythro-pent-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-ribonate + oxidized carboxamido-methyl viologen
D-erythro-pent-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
D-xylonate + oxidized anthraquinone-2,6-disulfonate
D-threo-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
D-xylonate + oxidized benzyl viologen
D-threo-pent-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
D-xylonate + oxidized carboxamido-methyl viologen
D-threo-pent-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
galactarate + oxidized anthraquinone-2,6-disulfonate
2-oxogalactarate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
galactarate + oxidized benzyl viologen
2-oxogalactarate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
galactarate + oxidized carboxamido-methyl viologen
2-oxogalactarate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
indolylpyruvate + reduced benzyl viologen
2-hydroxy-3-indolylpropionat + oxidized benzyl viologen
show the reaction diagram
indolylpyruvate + reduced methyl viologen
2-hydroxy-3-indolylpropionat + oxidized methyl viologen
show the reaction diagram
L-arabinonate + oxidized anthraquinone-2,6-disulfonate
L-erythro-pent-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
L-arabinonate + oxidized benzyl viologen
L-erythro-pent-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
L-arabinonate + oxidized carboxamido-methyl viologen
L-erythro-pent-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
L-arabinuronate + oxidized anthraquinone-2,6-disulfonate
? + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
L-arabinuronate + oxidized benzyl viologen
? + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
L-arabinuronate + oxidized carboxamido-methyl viologen
? + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
L-mannonate + oxidized anthraquinone-2,6-disulfonate
L-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
L-mannonate + oxidized benzyl viologen
L-arabino-hex-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
L-mannonate + oxidized carboxamido-methyl viologen
L-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
lactate + oxidized acceptor
pyruvate + reduced acceptor
show the reaction diagram
-
-
-
-
r
lactobionate + oxidized anthraquinone-2,6-disulfonate
4-O-(beta-D-glactopyranosido)-D-arabino-hex-2-ulosonate + reduced anthraquinone-2,6-disulfonate
show the reaction diagram
-
-
-
-
r
lactobionate + oxidized benzyl viologen
4-O-(beta-D-glactopyranosido)-D-arabino-hex-2-ulosonate + reduced benzyl viologen
show the reaction diagram
-
-
-
-
r
lactobionate + oxidized carboxamido-methyl viologen
4-O-(beta-D-glactopyranosido)-D-arabino-hex-2-ulosonate + reduced carboxamido-methyl viologen
show the reaction diagram
-
-
-
-
r
mandelate + oxidized acceptor
oxo(phenyl)acetic acid + reduced acceptor
show the reaction diagram
-
-
-
-
r
N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen
? + reduced 1,1'-carbamoyl methyl viologen
show the reaction diagram
-
anaerobic conditions
-
-
r
pantolactone + oxidized acceptor
dehydropantolactone + reduced acceptor
show the reaction diagram
-
-
-
-
r
phenylglyoxylate + reduced benzyl viologen
(R)-mandelic acid
show the reaction diagram
phenylglyoxylate + reduced methyl viologen
(R)-mandelic acid
show the reaction diagram
phenylpyruvate + H2
(2R)-phenyllactate
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen
phenyllactate + oxidized 1,1'-carbamoyl methyl viologen
show the reaction diagram
-
anaerobic conditions
-
-
r
phenylpyruvate + reduced benzyl viologen
(2R)-phenyllactate + oxidized benzyl viologen
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced benzyl viologen
2-hydroxy-3-phenylpropionate + oxidized benzyl viologen
show the reaction diagram
phenylpyruvate + reduced benzyl viologen
phenyllactate + oxidized benzyl viologen
show the reaction diagram
-
anaerobic conditions
-
-
r
phenylpyruvate + reduced carbamoylmethyl viologen
(2R)-phenyllactate + oxidized carbamoylmethyl viologen
show the reaction diagram
phenylpyruvate + reduced cobalt sepulchrate
(2R)-phenyllactate + oxidized cobalt sepulchrate
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced indigocarmine
(2R)-phenyllactate + oxidized indigocarmine
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced kresyl violet
(2R)-phenyllactate + oxidized kresyl violet
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced meldolablue
(2R)-phenyllactate + oxidized meldolablue
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced methyl viologen
2-hydroxy-3-phenylpropionate + oxidized methyl viologen
show the reaction diagram
phenylpyruvate + reduced methylene blue
(2R)-phenyllactate + oxidized methylene blue
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced phenosafranine
(2R)-phenyllactate + oxidized phenosafranine
show the reaction diagram
-
-
-
-
r
phenylpyruvate + reduced safranine TH
(2R)-phenyllactate + oxidized safranine TH
show the reaction diagram
-
-
-
-
r
pyruvate + reduced benzyl viologen
lactate + oxidized benzyl viologen
show the reaction diagram
pyruvate + reduced methyl viologen
lactate + oxidized methyl viologen
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
molybdenum cofactor
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron-sulfur cluster
-
-
molybdate
-
strong induction
Molybdenum
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
O2
-
oxygen-sensitive
tungstate
-
complete inhibition in vivo
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
highest activity in potassium phosphate buffer compared to Tris-buffer, MOPS, Pipes or diphosphate buffer
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.64
6-phospho-D-gluconate
-
pH 8.5, 38C
0.042
benzyl viologen
-
pH 8.5, 38C
0.025
carboxamido-methyl viologen
-
pH 8.5, 38C
0.48
D-galactonate
-
pH 8.5, 38C
1.68
D-glucarate
-
pH 8.5, 38C
0.98
D-gluconate
-
pH 8.5, 38C
0.64
D-gulonate
-
pH 8.5, 38C
0.68
D-lactate
-
pH 8.5, 38C
0.3
D-ribonate
-
pH 8.5, 38C
1.27
D-xylonate
-
pH 8.5, 38C
0.42
galactarate
-
pH 8.5, 38C
2.03
L-arabinoic acid
-
pH 8.5, 38C
1.46
L-arabinuronate
-
pH 8.5, 38C
0.92
L-mannonate
-
pH 8.5, 38C
0.66
Lactobionate
-
pH 8.5, 38C
0.19
oxidized antraquinone-2,6-disulfonate
-
pH 8.5, 38C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
600
-
purified enzyme
810
-
crude cell extract
1000
-
purified enzyme
1800
-
purified holoenzyme
additional information
-
production rates of D-(S)-chloroacetic acid by enzymatic and chemical or eletrochemical methods
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
35
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
600000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
-
8 * 80000, SDS-PAGE
oligomer
-
x * 80000 + x * 64000, alpha,beta-structure
additional information
-
subunit structure
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, loss of 10% activity within 1-2 years
-
-16C, N2-atmosphere, wet packed cells, 450 days, more than 90% remaining activity
-
30C, 170 h, completely stable
-
room temperature, freeze dried cells, 200 days, more than 80% remaining activity
-
room temperature, freeze dried cells, loss of 10% of activity within 1-2 years
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anaerobic conditions
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
preparative scale production of pyruvate from (R)-lactate in an enzyme-membrane reactor with coupled electrochemical regeneration of the artificial mediator anthraquinone-2,6-disulfonate, process modeling and calculation
synthesis