Information on EC 1.1.99.9 - pyridoxine 5-dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.99.9
-
RECOMMENDED NAME
GeneOntology No.
pyridoxine 5-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pyridoxine + acceptor = isopyridoxal + reduced acceptor
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
-
redox reaction
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-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Vitamin B6 metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
pyridoxine:acceptor 5-oxidoreductase
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9023-39-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Cr-7, inducible enzyme
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-
Manually annotated by BRENDA team
IA
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-
Manually annotated by BRENDA team
IA
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-deoxypyridoxine + acceptor
3-hydroxy-2-methylpyridine-5-carboxaldehyde + reduced acceptor
show the reaction diagram
-
18% of activity with pyridoxine
-
?
pyridoxal + acceptor
3-hydroxy-2-methylpyridine-4,5-dicarbaldehyde + reduced acceptor
show the reaction diagram
-
29% of activity with pyridoxine
-
?
pyridoxamine + acceptor
4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
show the reaction diagram
pyridoxine + acceptor
isopyridoxal + reduced acceptor
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxine + acceptor
isopyridoxal + reduced acceptor
show the reaction diagram
-
utilization of pyridoxine as sole source of carbon and nitrogen
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-pyridoxic acid
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-
Dithionitrobenzoic acid
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-
Isopyridoxal
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-
N-ethylmaleimide
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-
p-chloromercuribenzoate
pyridoxal
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-
pyridoxamine
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-
sulfhydryl reagents
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-
additional information
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not inhibited by alpha,alpha'-dipyridyl, o-, m- or p-phenanthroline
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28
O2
-
-
0.18 - 0.48
pyridoxine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
pyridoxine
Arthrobacter sp.
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acceptor O2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.3
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pyridoxine + O2, Tris buffer, phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
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pH 5.0: about 30% of activity maximum, pH 7.0: 17% of activity maximum
6.4 - 9.5
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activity at pH 9.5 in Tris is about 70% of that at pH 7.3 while activities at pH 6.4 and pH 6.0 in phosphate buffer are about 75% and 0% respectively of that at pH 7.3
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
112000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 58600, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE