Information on EC 1.1.99.B1 - flavin adenine dinucleotide-dependent 4-phospho-D-erythronate dehydrogenase

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The expected taxonomic range for this enzyme is: Sinorhizobium meliloti

EC NUMBER
COMMENTARY hide
1.1.99.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
flavin adenine dinucleotide-dependent 4-phospho-D-erythronate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-phospho-D-erythronate + FAD = 3-hydroxy-2-oxo-4-(phosphonooxy)butanoate + FADH2
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
4-phospho-D-erythronate:FAD 3-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
formerly Rhizobium meliloti, strain IFO 14782
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-phospho-D-erythronate + 2,6-dichloroindophenol +
3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + reduced 2,6-dichloroindophenol
show the reaction diagram
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-
-
-
?
4-phospho-D-erythronate + FAD
3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + FADH2
show the reaction diagram
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-
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.946
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0.091 mM DCIP and 0.00024 mg purified enzyme in 33 mM potassium phosphate buffer at pH 7.0
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
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SDS-PAGE
53190
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predicted from the pdxR DNA sequence
106000
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gel filtration chromatography
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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2 * 53000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Q Sepharose FF column chromatography and Sephadex LH 20 column chromatography
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