Information on EC 1.1.99.B6 - phosphonoacetaldehyde reductase (acceptor)

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
1.1.99.B6
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
phosphonoacetaldehyde reductase (acceptor)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-hydroxyethylphosphonate + acceptor = phosphonoacetaldehyde + reduced acceptor
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
2-hydroxyethylphosphonate:acceptor oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 144-91
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
complementation of fomC disrupted mutants with 2-hydroxyethylphosphonate
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-hydroxyethylphosphonate + NADP+
phosphonoacetaldehyde + NADPH + H+
show the reaction diagram
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r
phosphonoacetaldehyde + reduced acceptor
2-hydroxyethylphosphonate + acceptor
show the reaction diagram
fomC is required for production of fosfomycin
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-
?
additional information
?
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no reaction is observed with hydroxymethylphosphonate and 3-hydroxypropylphosphonate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphonoacetaldehyde + reduced acceptor
2-hydroxyethylphosphonate + acceptor
show the reaction diagram
D2SNG2
fomC is required for production of fosfomycin
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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FomC is an NADPH-dependent enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0098
NADPH
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in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication
0.031
phosphonoacetaldehyde
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in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3
phosphonoacetaldehyde
Streptomyces wedmorensis
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in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
41
phosphonoacetaldehyde
Streptomyces wedmorensis
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in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication
1309
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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phosphonoacetaldehyde reduction is more favorable under neutral conditions (optimal pH at 7.0)
9
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2-hydroxyethylphosphonate oxidation is favored under basic conditions (optimal pH at 9.0)
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon immobilized metal affinity chromatography and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of the fosfomycin biosynthetic gene cluster
expressed in Escherichia coli BL21(DE3) cells as His6-tagged enzyme
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