Information on EC 1.13.11.67 - 8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.67
-
RECOMMENDED NAME
GeneOntology No.
8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
8'-apo-beta-carotenol + O2 = 14'-apo-beta-carotenal + an uncharacterized product
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
8'-apo-beta-carotenol:O2 oxidoreductase (14',13'-cleaving)
A thiol-dependent enzyme isolated from rat and rabbit. Unlike EC 1.13.11.63, beta-carotene-15,15'-dioxygenase, it is not active towards beta-carotene. The secondary product has not been characterized, but may be (3E,5E)-7-hydroxy-6-methylhepta-3,5-dien-2-one.
CAS REGISTRY NUMBER
COMMENTARY hide
198028-39-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
rabbit
-
-
Manually annotated by BRENDA team
rat
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
show the reaction diagram
beta-apo-10'-carotenal + O2
retinal + ?
show the reaction diagram
-
-
-
?
beta-apo-8'-carotenal + O2
(2E,4E,6E,8E,10E)-5,9-dimethyl-11-(2,6,6-trimethylcyclohex-1-en-1-yl)undeca-2,4,6,8,10-pentaenal + (2E,4E)-2-methylhepta-2,4-dienedial
show the reaction diagram
-
-
-
?
beta-apo-8'-lycopenal + O2
crocetindial + ?
show the reaction diagram
-
main product
-
?
lycopene + O2
?
show the reaction diagram
-
eccentric cleavage close to the end of lycopene
-
?
torulene + O2
beta-apo-4'-carotenal + 3-methylbut-2-enal
show the reaction diagram
-
-
-
?
additional information
?
-
enzyme cleaves at the third double bond from the uncycled tail of carotenoids with preference for non-oxygenated tails
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergent
-
glutathione
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 11
8'-apo-beta-carotenol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000001
-
from liver
0.000002
-
from intestinal mucosa preparation
0.000003
-
from liver
0.00002
-
from intestinal mucosa preparation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G71D
mutant accumulates torulene instead of the final product of the pathway, neurosporaxanthin