Information on EC 1.14.11.49 - uridine-5'-phosphate dioxygenase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
1.14.11.49
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RECOMMENDED NAME
GeneOntology No.
uridine-5'-phosphate dioxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UMP + 2-oxoglutarate + O2 = 5'-dehydrouridine + succinate + CO2 + phosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UMP,2-oxoglutarate:oxygen oxidoreductase
The enzyme catalyses a net dephosphorylation and oxidation of UMP to generate 5'-dehydrouridine, the first intermediate in the biosynthesis of the unusual aminoribosyl moiety found in several C7-furanosyl nucleosides such as A-90289s, caprazamycins, liposidomycins, muraymycins and FR-900453. Requires Fe2+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Streptomyces sp. SANK60405
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UMP + 2-oxoglutarate + O2
5'-dehydrouridine + succinate + CO2 + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UMP + 2-oxoglutarate + O2
5'-dehydrouridine + succinate + CO2 + phosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
does not contain heme
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
not influenced by Ca2+, K+ and Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
less than 15% activity at 1 mM
Cu2+
-
less than 5% activity at 1 mM
Fe3+
-
less than 10% activity at 1 mM
Mn2+
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less than 15% activity at 1 mM
Ni2+
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20% residual activity at 1 mM
Zn2+
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less than 1% activity at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
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1 mM activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0075
2-oxoglutarate
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at pH 7.5 and 30C
0.014
UMP
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at pH 7.5 and 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.53
2-oxoglutarate
Streptomyces sp.
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at pH 7.5 and 30C
1.27
UMP
Streptomyces sp.
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at pH 7.5 and 30C
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA resin column chromatography
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nickel-nitrilotriacetic acid-agarose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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