Information on EC 1.14.12.16 - 2-Hydroxyquinoline 5,6-dioxygenase

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The expected taxonomic range for this enzyme is: Comamonas testosteroni

EC NUMBER
COMMENTARY hide
1.14.12.16
-
RECOMMENDED NAME
GeneOntology No.
2-Hydroxyquinoline 5,6-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
quinolin-2-ol + NADH + H+ + O2 = 2,5,6-trihydroxy-5,6-dihydroquinoline + NAD+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-methylquinoline degradation
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-
SYSTEMATIC NAME
IUBMB Comments
quinolin-2-ol,NADH:oxygen oxidoreductase (5,6-hydroxylating)
3-Methylquinolin-2-ol, quinolin-8-ol and quinolin-2,8-diol are also substrates. Quinolin-2-ols exist largely as their quinolin-2(1H)-one tautomers
CAS REGISTRY NUMBER
COMMENTARY hide
172399-50-7
not distinguished from EC 1.14.13.65
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 63
-
-
Manually annotated by BRENDA team
strain 63
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Oxo-1,2-dihydroquinoline + NADH + O2
?
show the reaction diagram
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
show the reaction diagram
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
?
show the reaction diagram
8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
?
show the reaction diagram
-
-
-
-
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8-Hydroxyquinoline + NADH + O2
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
required, cannot be replaced by NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
enhances activity 1.5fold
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
0.5 mM, 82% loss of activity
4-hydroxymercuribenzoate
-
0.1 mM, complete loss of activity
Acriflavin
-
0.5 mM, 69% loss of activity
diethyldithiocarbaminate
-
inhibits after a prolonged incubation time
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EDTA
-
inhibits after a prolonged incubation time
iodoacetate
-
2 mM, complete loss of activity
NaCl
-
0.2 M, 50% loss of activity
Quinacrine
-
0.5 mM, 15% loss of activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
5 min, 30% loss of activity
65
-
5 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethanol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE