Information on EC 1.14.12.5 - 5-pyridoxate dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.12.5
-
RECOMMENDED NAME
GeneOntology No.
5-pyridoxate dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + H+ + O2 = 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+
show the reaction diagram
A flavoprotein
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Vitamin B6 metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
5-pyridoxate,NADPH:oxygen oxidoreductase (decyclizing)
A flavoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-70-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Cr7
-
-
Manually annotated by BRENDA team
strain MA-1
-
-
Manually annotated by BRENDA team
strain MA-1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
biodegradation of vitamin B6 in bacteria
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxy-2-methylpyridine-5-carboxylic acid + NADH
2-[(acetylamino)methylene]succinate + NAD+ + H2O
show the reaction diagram
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2
2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O
show the reaction diagram
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+
2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O
show the reaction diagram
5-pyridoxic acid + NADPH + O2 + H3O+
2-(acetylaminomethylene)succinate + NADP+ + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+
2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-pyridoxic acid
-
-
6-methylnicotinic acid
-
-
p-chloromercuribenzoate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
5-pyridoxate
-
-
0.01
NADPH
-
-
0.125
O2
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
5-pyridoxic acid
-
-
0.2
6-methylnicotinic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
broad pH-optimum in phosphate, diphosphate and Tris buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.6
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
native enzyme, gel filtration
39200
-
native enzyme, sedimentation velocity
51000
-
SDS-PAGE
166000
-
sedimentation equlibrium
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity is lost on resolution with acidic ammonium sulfate and can be completely restored with FAD+, but not with FMN
-
highly sensitive to sulfhydryl reagents but not to chelating agents and is stabilized by high concentrations of mercaptoethanol and glycerol
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
highly sensitive to oxidation
-
439054
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, can be stored up to 1 month with loss about 20% of its activity in a mixture of 50% glycerol and 0.1% 2-mercaptoethanol
-
4°C, loses activity rapidly in absence of mercaptoethanol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE