Information on EC 1.14.13.146 - taxoid 14beta-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Taxus

EC NUMBER
COMMENTARY hide
1.14.13.146
-
RECOMMENDED NAME
GeneOntology No.
taxoid 14beta-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + O2 + NADPH + H+ = 10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
taxol biosynthesis
-
-
Diterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5alpha-acetoxy-10beta-hydroxytaxadiene + O2 + NADPH + H+
5alpha-acetoxy-10beta,14beta-dihydroxytaxadiene + NADP+ + H2O
show the reaction diagram
-
-
-
?
5alpha-acetoxytaxadiene + O2 + NADPH + H+
? + NADP+ + H2O
show the reaction diagram
-
-
-
?
5alpha-hydroxytaxa-4(20),11(12)-diene + O2 + NADPH + H+
? + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.055
5alpha-acetoxy-10beta-hydroxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
0.033
5alpha-acetoxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 57146, calculated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae WAT11 cells
expressed in the Taxus x media TM3 cell line. The expression of endogenous taxoid 14beta-hydroxylase gene is significantly suppressed by the exogenous as taxoid 14beta-hydroxylase gene
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
an antisense suppression approach, repressing the expression of the taxoid 14beta-hydroxlyase gene in yew cell cultures, is useful to inhibit the expression of other important genes in side-route of Taxol pathway and this may diverts the flow of taxadiene mainly towards Taxol
medicine
manipulation of the hydroxylase gene can permit redirection of the pathway to increase flux toward taxol and can allow the preparation of 13alpha,14beta-hydroxy taxoids as new therapeutic agents