Information on EC 1.14.13.189 - 5-methyl-1-naphthoate 3-hydroxylase

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The expected taxonomic range for this enzyme is: Streptomyces sahachiroi

EC NUMBER
COMMENTARY hide
1.14.13.189
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RECOMMENDED NAME
GeneOntology No.
5-methyl-1-naphthoate 3-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-methyl-1-naphthoate + NADPH + H+ + O2 = 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
5-methyl-1-naphthoate,NADPH:oxygen oxidoreductase (3-hydroxylating)
The enzyme from the bacterium Streptomyces sahachiroi is involved in the biosynthesis of 3-methoxy-5-methyl-1-naphthoate, a component of of the the antitumor antibiotic azinomycin B.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-methyl-1-naphthoate + NADPH + H+ + O2
3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-methyl-1-naphthoate + NADPH + H+ + O2
3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
show the reaction diagram
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the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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a heme-thiolate protein (P450)
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.4
NADPH
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pH 9.0, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
NADPH
Streptomyces sahachiroi
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pH 9.0, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0054
NADPH
Streptomyces sahachiroi
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pH 9.0, 25°C
5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
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pH 7.0: about 45% of maximal activity, pH 10.0: about 80% of maximal activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Streptomyces albus or in Escherichia coliBö21(DE3)
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