Information on EC 1.14.13.198 - monacolin L hydroxylase

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The expected taxonomic range for this enzyme is: Aspergillus terreus

EC NUMBER
COMMENTARY hide
1.14.13.198
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RECOMMENDED NAME
GeneOntology No.
monacolin L hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
monacolin L acid + O2 + NADPH + H+ = monacolin J acid + NADP+ + H2O
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lovastatin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
monacolin L acid,NADPH:oxygen oxidoreductase (8-hydroxylating)
A heme-thiolate protein (cytochrome P-450). The enzyme from fungi also catalyses the reaction of EC 1.14.13.197, dihydromonacolin L hydroxylase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
monacolin L acid + O2 + NADPH + H+
monacolin J acid + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
monacolin L acid + O2 + NADPH + H+
monacolin J acid + NADP+ + H2O
show the reaction diagram
Q9Y7C8
monacolin L acid i.e. (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid. The enzyme also catalyses the reaction of EC 1.14.13.197, dihydromonacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin
monacolin J acid i.e. (3R,5R)-7-[(1S,2S,6R,8S,8aR)-8-hydroxy-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P-450
a heme-thiolate protein
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0062
monacolin L acid
pH and temperature not specified in the publication
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the lovA gene in C-terminal FLAG-tagged form is cloned in the same vector coding cpr (cytochrome P450 oxidoreductase), and both lovA and cpr are co-expressed in Saccharomyces cerevisiae