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IUBMB CommentsThe enzyme, characterized from the bacterium Thauera butanivorans, is similar to EC 1.14.13.25, methane monooxygenase (soluble), but has a very low activity with methane. It comprises three components - a carboxylate-bridged non-heme di-iron center-containing hydroxylase (made of three different subunits), a flavo-iron sulfur-containing NADH-oxidoreductase, and a small regulatory component protein. The enzyme can also act on other C3-C6 linear and branched aliphatic alkanes with lower activity.
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butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
butane + NADH + H+ + O2
butan-2-ol + NAD+ + H2O
ethane + NADH + H+ + O2
ethanol + NAD+ + H2O
hexane + NADH + H+ + O2
hexan-1-ol + NAD+ + H2O
isobutane + NADH + H+ + O2
2-methyl-1-propanol + NAD+ + H2O
isopentane + NADH + H+ + O2
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
methane + NADH + H+ + O2
methanol + NAD+ + H2O
pentane + NADH + H+ + O2
pentan-1-ol + NAD+ + H2O
pentane + NADH + H+ + O2
pentan-2-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
pentane + NADH + H+ + O2
pentan-3-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
propane + NADH + H+ + O2
propan-1-ol + NAD+ + H2O
propane + NADH + H+ + O2
propan-2-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
additional information
?
-
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
-
-
-
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7
-
formation of about 3.6% of butan-2-ol
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
-
-
-
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB
-
?
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
butane + NADH + H+ + O2
butan-2-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
butane + NADH + H+ + O2
butan-2-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
ethane + NADH + H+ + O2
ethanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
ethane + NADH + H+ + O2
ethanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
hexane + NADH + H+ + O2
hexan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively
-
?
hexane + NADH + H+ + O2
hexan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively
-
?
isobutane + NADH + H+ + O2
2-methyl-1-propanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively
-
?
isobutane + NADH + H+ + O2
2-methyl-1-propanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively
-
?
isopentane + NADH + H+ + O2
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively
-
?
isopentane + NADH + H+ + O2
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively
-
?
methane + NADH + H+ + O2
methanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
methane + NADH + H+ + O2
methanol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
pentane + NADH + H+ + O2
pentan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
pentane + NADH + H+ + O2
pentan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 80% pentan-1-ol plus 13% pentan-2-ol and 7% pentan-3-ol in absence, and 88% pentan-1-ol plus 11% pentan-2-ol and 1% pentan-3-ol in presence of regulatory subunit BmoB, respectively
-
?
pentane + NADH + H+ + O2
pentan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
propane + NADH + H+ + O2
propan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
-
-
?
propane + NADH + H+ + O2
propan-1-ol + NAD+ + H2O
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
-
about 82% propan-1-ol plus 18% propan-2-ol, in presence and absence of regulatory subunit BmoB
-
?
additional information
?
-
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene
-
-
?
additional information
?
-
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene
-
-
?
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0.76
ethane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
pH 7.2, 25°C
1.3
methane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
pH 7.2, 25°C
0.12
butane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product butan-1-ol, pH 7.2, 25°C
0.48
butane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product butan-1-ol, pH 7.2, 25°C
0.008
pentane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product pentan-3-ol, pH 7.2, 25°C
0.039
pentane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product pentan-2-ol, pH 7.2, 25°C
0.34
pentane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product pentan-1-ol, pH 7.2, 25°C
0.55
propane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product propan-1-ol, pH 7.2, 25°C
0.98
propane
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
product propan-2-ol, pH 7.2, 25°C
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L287F
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX, loss of activity
P179K
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX, loss of activity
Q319G
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX, loss of activity
V181M
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX, loss of activity
F321Y
-
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 61% residual activity
-
G113N
-
mutation in the alpha-subunit of hydrolase component, loss of inhibition by propanoate
-
L279F
-
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 56% residual activity
-
Q320K
-
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 28% residual activity
-
T148C
-
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 15% residual activity
-
F321Y
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX towards soluble methane monooxygenase, no formation of butan-2-ol
F321Y
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 61% residual activity
G113N
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 11.1
G113N
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
mutation in the alpha-subunit of hydrolase component, loss of inhibition by propanoate
L279F
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.22
L279F
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 56% residual activity
Q320K
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.20
Q320K
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 28% residual activity
T148C
Q8KQF0; Q8KQE9; Q8KQE7
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.15
T148C
Q8KQF0; Q8KQE9; Q8KQE7; Q8KQE6; Q8KQE8
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 15% residual activity
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Sayavedra-Soto, L.A.; Doughty, D.M.; Kurth, E.G.; Bottomley, P.J.; Arp, D.J.
Product and product-independent induction of butane oxidation in Pseudomonas butanovora
FEMS Microbiol. Lett.
250
111-116
2005
Thauera butanivorans, Thauera butanivorans ATCC 43655
brenda
Halsey, K.H.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.
Site-directed amino acid substitutions in the hydroxylase alpha subunit of butane monooxygenase from Pseudomonas butanovora: Implications for substrates knocking at the gate
J. Bacteriol.
188
4962-4969
2006
Thauera butanivorans (Q8KQF0 and Q8KQE9 and Q8KQE7)
brenda
Sluis, M.K.; Sayavedra-Soto, L.A.; Arp, D.J.
Molecular analysis of the soluble butane monooxygenase from Pseudomonas butanovora
Microbiology
148
3617-3629
2002
Thauera butanivorans (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8), Thauera butanivorans ATCC 43655 (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8)
brenda
Dubbels, B.L.; Sayavedra-Soto, L.A.; Arp, D.J.
Butane monooxygenase of Pseudomonas butanovora: purification and biochemical characterization of a terminal-alkane hydroxylating diiron monooxygenase
Microbiology
153
1808-1816
2007
Thauera butanivorans (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8), Thauera butanivorans ATCC 43655 (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8)
brenda
Doughty, D.M.; Halsey, K.H.; Vieville, C.J.; Sayavedra-Soto, L.A.; Arp, D.J.; Bottomley, P.J.
Propionate inactivation of butane monooxygenase activity in Pseudomonas butanovora: biochemical and physiological implications
Microbiology
153
3722-3729
2007
Thauera butanivorans (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8), Thauera butanivorans ATCC 43655 (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8)
brenda
Cooley, R.B.; Dubbels, B.L.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.
Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly Pseudomonas butanovora
Microbiology
155
2086-2096
2009
Thauera butanivorans (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8), Thauera butanivorans ATCC 43655 (Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8)
brenda